Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics
MicroRNAs are small noncoding RNA molecules that control expression of target genes. Our previous studies show that let-7a decreased in gastric carcinoma and that up-regulation of let-7a by gene augmentation inhibited gastric carcinoma cell growth both in vitro and in vivo, whereas it remains largel...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-2012-0063 |
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| author | Yimin Zhu Xingyuan Xiao Lairong Dong Zhiming Liu |
| author_facet | Yimin Zhu Xingyuan Xiao Lairong Dong Zhiming Liu |
| author_sort | Yimin Zhu |
| collection | DOAJ |
| description | MicroRNAs are small noncoding RNA molecules that control expression of target genes. Our previous studies show that let-7a decreased in gastric carcinoma and that up-regulation of let-7a by gene augmentation inhibited gastric carcinoma cell growth both in vitro and in vivo, whereas it remains largely unclear as to how let-7a affects tumor growth. In this study, proteins associated with the function of let-7a were detected by high throughout screening. The cell line of SGC-7901 stablely overexpressing let-7a was successfully established by gene cloning. Two-dimensional gel electrophoresis (2-DEy was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. Ten different protein spots were identified by MALDI-TOF-MS, and they may be the proteins associated with let-7a function. The overexpressed proteins included Antioxidant protein 2, Insulin–like growth factor binding protein 2, Protein disulfide isomerase A2, C-1-tetrahydrofolate synthase, Cyclin-dependent kinase inhibitor1 (CDKN1) and Rho–GTPase activating protein 4. The underexpressed proteins consisted of S-phase kinase-associated protein 2 (Spk2), Platelet membrane glycoprotein, Fibronectin and Cks1 protein. Furthermore, the different expression levels of the partial proteins (CDKN1, Spk2 and Fibronectin) were confirmed by western blot analysis. The data suggest that these differential proteins are involved in a novel let-7a signal pathway and these findings provide the basis to investigate the functional mechanisms of let-7a in gastric carcinoma. |
| format | Article |
| id | doaj-art-d992393ad9ba4186a64be882659df9d6 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-d992393ad9ba4186a64be882659df9d62025-02-03T01:25:24ZengWileyAnalytical Cellular Pathology2210-71772210-71852012-01-0135428529510.3233/ACP-2012-0063Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by ProteomicsYimin Zhu0Xingyuan Xiao1Lairong Dong2Zhiming Liu3Department of Oncology, The Second Hospital of Jiaxing, Jiaxing, ChinaDepartment of General Surgery, The First People's Hospital of Kunshan, Kunshan, ChinaDepartment of Oncology, The Second Hospital of Jiaxing, Jiaxing, ChinaGastrointestine and Gland Surgery Division, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaMicroRNAs are small noncoding RNA molecules that control expression of target genes. Our previous studies show that let-7a decreased in gastric carcinoma and that up-regulation of let-7a by gene augmentation inhibited gastric carcinoma cell growth both in vitro and in vivo, whereas it remains largely unclear as to how let-7a affects tumor growth. In this study, proteins associated with the function of let-7a were detected by high throughout screening. The cell line of SGC-7901 stablely overexpressing let-7a was successfully established by gene cloning. Two-dimensional gel electrophoresis (2-DEy was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. Ten different protein spots were identified by MALDI-TOF-MS, and they may be the proteins associated with let-7a function. The overexpressed proteins included Antioxidant protein 2, Insulin–like growth factor binding protein 2, Protein disulfide isomerase A2, C-1-tetrahydrofolate synthase, Cyclin-dependent kinase inhibitor1 (CDKN1) and Rho–GTPase activating protein 4. The underexpressed proteins consisted of S-phase kinase-associated protein 2 (Spk2), Platelet membrane glycoprotein, Fibronectin and Cks1 protein. Furthermore, the different expression levels of the partial proteins (CDKN1, Spk2 and Fibronectin) were confirmed by western blot analysis. The data suggest that these differential proteins are involved in a novel let-7a signal pathway and these findings provide the basis to investigate the functional mechanisms of let-7a in gastric carcinoma.http://dx.doi.org/10.3233/ACP-2012-0063 |
| spellingShingle | Yimin Zhu Xingyuan Xiao Lairong Dong Zhiming Liu Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics Analytical Cellular Pathology |
| title | Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics |
| title_full | Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics |
| title_fullStr | Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics |
| title_full_unstemmed | Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics |
| title_short | Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics |
| title_sort | investigation and identification of let 7a related functional proteins in gastric carcinoma by proteomics |
| url | http://dx.doi.org/10.3233/ACP-2012-0063 |
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