circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD
Endothelial-to-mesenchymal transition (EndMT) is a pivotal event in diabetic retinopathy (DR). This study explored the role of circRNA zinc finger protein 532 (circZNF532) in regulating EndMT in DR progression. Human retinal microvascular endothelial cells (HRMECs) were exposed to high glucose (HG)...
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The Japan Endocrine Society
2024-07-01
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| Series: | Endocrine Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/71/7/71_EJ23-0683/_html/-char/en |
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| author | Xiao-Lin Fu Fu-Tao He Mo-Han Li Chun-Yan Fu Jian-Zhi Chen |
| author_facet | Xiao-Lin Fu Fu-Tao He Mo-Han Li Chun-Yan Fu Jian-Zhi Chen |
| author_sort | Xiao-Lin Fu |
| collection | DOAJ |
| description | Endothelial-to-mesenchymal transition (EndMT) is a pivotal event in diabetic retinopathy (DR). This study explored the role of circRNA zinc finger protein 532 (circZNF532) in regulating EndMT in DR progression. Human retinal microvascular endothelial cells (HRMECs) were exposed to high glucose (HG) to induce the DR cell model. Actinomycin D-treated HRMECs were used to confirm the mRNA stability of phosphoinositide-3 kinase catalytic subunit δ (PIK3CD). The interaction between TATA-box-binding protein-associated factor 15 (TAF15) and circZNF532/PIK3CD was subsequently analyzed using RNA immunoprecipitation (RIP), RNA pull-down. It was found that HG treatment accelerated EndMT process, facilitated cell migration and angiogenesis, and enhanced PIK3CD and p-AKT levels in HRMECs, whereas si-circZNF532 transfection neutralized these effects. Further data showed that circZNF532 recruited TAF15 to stabilize PIK3CD, thus elevating PIK3CD expression. Following rescue experiments suggested that PIK3CD overexpression partially negated the inhibitory effect of circZNF532 silencing on EndMT, migration, and angiogenesis of HG-treated HRMECs. In conclusion, our results suggest that circZNF532 recruits TAF15 to stabilize PIK3CD, thereby facilitating EndMT in DR. |
| format | Article |
| id | doaj-art-d98e612c185347c7ace909af21835809 |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-d98e612c185347c7ace909af218358092025-01-22T05:12:18ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402024-07-0171767568610.1507/endocrj.EJ23-0683endocrjcircZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CDXiao-Lin Fu0Fu-Tao He1Mo-Han Li2Chun-Yan Fu3Jian-Zhi Chen4Department of Ophthalmology, Hainan West Central Hospital, Danzhou 571700, Hainan Province, P.R. ChinaDepartment of Ophthalmology, Hainan West Central Hospital, Danzhou 571700, Hainan Province, P.R. ChinaDepartment of Ophthalmology, Hainan West Central Hospital, Danzhou 571700, Hainan Province, P.R. ChinaDepartment of Ophthalmology, Hainan West Central Hospital, Danzhou 571700, Hainan Province, P.R. ChinaDepartment of Ophthalmology, Hainan West Central Hospital, Danzhou 571700, Hainan Province, P.R. ChinaEndothelial-to-mesenchymal transition (EndMT) is a pivotal event in diabetic retinopathy (DR). This study explored the role of circRNA zinc finger protein 532 (circZNF532) in regulating EndMT in DR progression. Human retinal microvascular endothelial cells (HRMECs) were exposed to high glucose (HG) to induce the DR cell model. Actinomycin D-treated HRMECs were used to confirm the mRNA stability of phosphoinositide-3 kinase catalytic subunit δ (PIK3CD). The interaction between TATA-box-binding protein-associated factor 15 (TAF15) and circZNF532/PIK3CD was subsequently analyzed using RNA immunoprecipitation (RIP), RNA pull-down. It was found that HG treatment accelerated EndMT process, facilitated cell migration and angiogenesis, and enhanced PIK3CD and p-AKT levels in HRMECs, whereas si-circZNF532 transfection neutralized these effects. Further data showed that circZNF532 recruited TAF15 to stabilize PIK3CD, thus elevating PIK3CD expression. Following rescue experiments suggested that PIK3CD overexpression partially negated the inhibitory effect of circZNF532 silencing on EndMT, migration, and angiogenesis of HG-treated HRMECs. In conclusion, our results suggest that circZNF532 recruits TAF15 to stabilize PIK3CD, thereby facilitating EndMT in DR.https://www.jstage.jst.go.jp/article/endocrj/71/7/71_EJ23-0683/_html/-char/endiabetic retinopathy (dr)human retinal microvascular endothelial cells (hrmecs)endothelial-to-mesenchymal transition (endmt)circrna zinc finger protein 532 (circznf532)phosphoinositide-3 kinase catalytic subunit δ (pik3cd) |
| spellingShingle | Xiao-Lin Fu Fu-Tao He Mo-Han Li Chun-Yan Fu Jian-Zhi Chen circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD Endocrine Journal diabetic retinopathy (dr) human retinal microvascular endothelial cells (hrmecs) endothelial-to-mesenchymal transition (endmt) circrna zinc finger protein 532 (circznf532) phosphoinositide-3 kinase catalytic subunit δ (pik3cd) |
| title | circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD |
| title_full | circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD |
| title_fullStr | circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD |
| title_full_unstemmed | circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD |
| title_short | circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD |
| title_sort | circznf532 promotes endothelial to mesenchymal transition in diabetic retinopathy by recruiting taf15 to stabilize pik3cd |
| topic | diabetic retinopathy (dr) human retinal microvascular endothelial cells (hrmecs) endothelial-to-mesenchymal transition (endmt) circrna zinc finger protein 532 (circznf532) phosphoinositide-3 kinase catalytic subunit δ (pik3cd) |
| url | https://www.jstage.jst.go.jp/article/endocrj/71/7/71_EJ23-0683/_html/-char/en |
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