The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases
IntroductionDiffuse parenchymal lung diseases (DPLD) cover heterogeneous types of lung disorders. Among many pathological phenotypes, pulmonary fibrosis is the most devastating and represents a characteristic sign of idiopathic pulmonary fibrosis (IPF). Despite a poor prognosis brought by pulmonary...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1479458/full |
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| author | Magda Suchankova Magda Suchankova Eszter Zsemlye Eszter Zsemlye Jan Urban Peter Baráth Lenka Kohútová Barbara Siváková Barbara Siváková Martina Ganovska Elena Tibenska Kinga Szaboova Eva Tedlova Dominik Juskanic Dominik Juskanic Kristina Kluckova Kristina Kluckova Michaela Kardohelyova Tetiana Moskalets Anna Ohradanova-Repic Patrik Babulic Maria Bucova Vladimir Leksa |
| author_facet | Magda Suchankova Magda Suchankova Eszter Zsemlye Eszter Zsemlye Jan Urban Peter Baráth Lenka Kohútová Barbara Siváková Barbara Siváková Martina Ganovska Elena Tibenska Kinga Szaboova Eva Tedlova Dominik Juskanic Dominik Juskanic Kristina Kluckova Kristina Kluckova Michaela Kardohelyova Tetiana Moskalets Anna Ohradanova-Repic Patrik Babulic Maria Bucova Vladimir Leksa |
| author_sort | Magda Suchankova |
| collection | DOAJ |
| description | IntroductionDiffuse parenchymal lung diseases (DPLD) cover heterogeneous types of lung disorders. Among many pathological phenotypes, pulmonary fibrosis is the most devastating and represents a characteristic sign of idiopathic pulmonary fibrosis (IPF). Despite a poor prognosis brought by pulmonary fibrosis, there are no specific diagnostic biomarkers for the initial development of this fatal condition. The major hallmark of lung fibrosis is uncontrolled activation of lung fibroblasts to myofibroblasts associated with extracellular matrix deposition and the loss of both lung structure and function.MethodsHere, we used this peculiar feature in order to identify specific biomarkers of pulmonary fibrosis in bronchoalveolar lavage fluids (BALF). The primary MRC-5 human fibroblasts were activated with BALF collected from patients with clinically diagnosed lung fibrosis; the activated fibroblasts were then washed rigorously, and further incubated to allow secretion. Afterwards, the secretomes were analysed by mass spectrometry.ResultsIn this way, the CD44 protein was identified; consequently, BALF of all DPLD patients were positively tested for the presence of CD44 by ELISA. Finally, biochemical and biophysical characterizations revealed an exosomal origin of CD44. Receiver operating characteristics curve analysis confirmed CD44 in BALF as a specific and reliable biomarker of IPF and other types of DPLD accompanied with pulmonary fibrosis. |
| format | Article |
| id | doaj-art-d98637da22f74b199a24ac8cfcdb03a4 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-d98637da22f74b199a24ac8cfcdb03a42025-01-13T05:10:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.14794581479458The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseasesMagda Suchankova0Magda Suchankova1Eszter Zsemlye2Eszter Zsemlye3Jan Urban4Peter Baráth5Lenka Kohútová6Barbara Siváková7Barbara Siváková8Martina Ganovska9Elena Tibenska10Kinga Szaboova11Eva Tedlova12Dominik Juskanic13Dominik Juskanic14Kristina Kluckova15Kristina Kluckova16Michaela Kardohelyova17Tetiana Moskalets18Anna Ohradanova-Repic19Patrik Babulic20Maria Bucova21Vladimir Leksa22Laboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaInstitute of Immunology, Faculty of Medicine Comenius University, Bratislava, SlovakiaLaboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaInstitute of Immunology, Faculty of Medicine Comenius University, Bratislava, SlovakiaNational Institute for Tuberculosis, Lung Diseases and Thoracic Surgery, Vysne Hagy, SlovakiaDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SlovakiaDepartment of Medical and Clinical Biophysics, Faculty of Medicine, Pavol Jozef Safarik University in Kosice, Kosice, SlovakiaNational Institute for Tuberculosis, Lung Diseases and Thoracic Surgery, Vysne Hagy, SlovakiaMedirex Ltd., Medirex Group Academy n.p.o., Bratislava, SlovakiaMedirex Ltd., Medirex Group Academy n.p.o., Bratislava, SlovakiaDepartment of Pneumology and Phthisiology, Faculty of Medicine Comenius University and University Hospital, Bratislava, SlovakiaJessenius Diagnostic Center, Nitra, SlovakiaFaculty of Medicine, Slovak Medical University, Bratislava, Slovakia0Clinic for Children and Adolescents, Faculty Hospital Nitra, Nitra, Slovakia1Hematology and Transfusiology Department, National Institute of Children’s Diseases and Medical Faculty, Comenius University, Bratislava, SlovakiaInstitute of Immunology, Faculty of Medicine Comenius University, Bratislava, SlovakiaLaboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia2Molecular Immunology Unit, Institute for Hygiene and Applied Immunology, Centre for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, AustriaLaboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaInstitute of Immunology, Faculty of Medicine Comenius University, Bratislava, SlovakiaLaboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, SlovakiaIntroductionDiffuse parenchymal lung diseases (DPLD) cover heterogeneous types of lung disorders. Among many pathological phenotypes, pulmonary fibrosis is the most devastating and represents a characteristic sign of idiopathic pulmonary fibrosis (IPF). Despite a poor prognosis brought by pulmonary fibrosis, there are no specific diagnostic biomarkers for the initial development of this fatal condition. The major hallmark of lung fibrosis is uncontrolled activation of lung fibroblasts to myofibroblasts associated with extracellular matrix deposition and the loss of both lung structure and function.MethodsHere, we used this peculiar feature in order to identify specific biomarkers of pulmonary fibrosis in bronchoalveolar lavage fluids (BALF). The primary MRC-5 human fibroblasts were activated with BALF collected from patients with clinically diagnosed lung fibrosis; the activated fibroblasts were then washed rigorously, and further incubated to allow secretion. Afterwards, the secretomes were analysed by mass spectrometry.ResultsIn this way, the CD44 protein was identified; consequently, BALF of all DPLD patients were positively tested for the presence of CD44 by ELISA. Finally, biochemical and biophysical characterizations revealed an exosomal origin of CD44. Receiver operating characteristics curve analysis confirmed CD44 in BALF as a specific and reliable biomarker of IPF and other types of DPLD accompanied with pulmonary fibrosis.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1479458/fulldiffuse parenchymal lung diseasespulmonary fibrosisbronchoalveolar lavage fluidsCD44exosomes |
| spellingShingle | Magda Suchankova Magda Suchankova Eszter Zsemlye Eszter Zsemlye Jan Urban Peter Baráth Lenka Kohútová Barbara Siváková Barbara Siváková Martina Ganovska Elena Tibenska Kinga Szaboova Eva Tedlova Dominik Juskanic Dominik Juskanic Kristina Kluckova Kristina Kluckova Michaela Kardohelyova Tetiana Moskalets Anna Ohradanova-Repic Patrik Babulic Maria Bucova Vladimir Leksa The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases Frontiers in Immunology diffuse parenchymal lung diseases pulmonary fibrosis bronchoalveolar lavage fluids CD44 exosomes |
| title | The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| title_full | The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| title_fullStr | The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| title_full_unstemmed | The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| title_short | The bronchoalveolar lavage fluid CD44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| title_sort | bronchoalveolar lavage fluid cd44 as a marker for pulmonary fibrosis in diffuse parenchymal lung diseases |
| topic | diffuse parenchymal lung diseases pulmonary fibrosis bronchoalveolar lavage fluids CD44 exosomes |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1479458/full |
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