Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice

This study aimed to evaluate the neuroprotective effects of the ethanolic leaf extract of Crassocephalum crepidioides (Cc) on diazepam-induced amnesia in mice. Thirty mice distributed into six groups of five mice each were used. The normal control and negative control groups received 2% ethanol per...

Full description

Saved in:
Bibliographic Details
Main Authors: Beppe Galba Jean, Folefack Alice Irène, Atsamo Albert Donatien, Ngatanko Aïbassou Hervé-Hervé, Barga Mpoo Bertrand, Allah-Doum Nanou Gael, Nguedia Ymele Merline, Zemo Gamo Franklik, Dongmo Alain Bertrand, Dimo Théophile
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Advances in Pharmacological and Pharmaceutical Sciences
Online Access:http://dx.doi.org/10.1155/2022/1919469
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832568177096130560
author Beppe Galba Jean
Folefack Alice Irène
Atsamo Albert Donatien
Ngatanko Aïbassou Hervé-Hervé
Barga Mpoo Bertrand
Allah-Doum Nanou Gael
Nguedia Ymele Merline
Zemo Gamo Franklik
Dongmo Alain Bertrand
Dimo Théophile
author_facet Beppe Galba Jean
Folefack Alice Irène
Atsamo Albert Donatien
Ngatanko Aïbassou Hervé-Hervé
Barga Mpoo Bertrand
Allah-Doum Nanou Gael
Nguedia Ymele Merline
Zemo Gamo Franklik
Dongmo Alain Bertrand
Dimo Théophile
author_sort Beppe Galba Jean
collection DOAJ
description This study aimed to evaluate the neuroprotective effects of the ethanolic leaf extract of Crassocephalum crepidioides (Cc) on diazepam-induced amnesia in mice. Thirty mice distributed into six groups of five mice each were used. The normal control and negative control groups received 2% ethanol per os, the positive control group received piracetam (150 mg/kg, p.o), and three experimental groups were treated with three doses of ethanolic leaf extract of Cc (100, 200, and 400 mg/kg, p.o). All groups except the normal control group were co-treated with diazepam (3 mg/kg, i.p) daily for 14 days. The memory effects were evaluated using the Radial Arm Maze (RAM) and the Novel Object Recognition (NOR) tests, while the anti-depressive effects were evaluated using the tail suspension test. All animals were sacrificed at the end of the study. Hippocampi, isolated from the right hemisphere, were used to prepare a homogenate for the determination of oxidative stress biomarkers. The ethanolic leaf extract of cc significantly (p<0.001) decreased the number of working and reference memory errors in the RAM test and induced a significant (p<0.01) increase in the time spent exploring the novel object in the NOR test. The extract also induced a significant (p<0.001) increase in the mobility time in tail suspension. Moreover, compared to the negative control group, the extract significantly (p<0.01) increased superoxide dismutase activity and significantly (p<0.01) decreased malondialdehyde levels. The histopathological analysis of hippocampi showed that the cc extract increased cell density when compared with the negative control. These results suggest that the ethanolic left extract of cc could have neuroprotective properties, which could be attributed to its antioxidant properties.
format Article
id doaj-art-d98507861c044d6d956f794e1e8157dd
institution Kabale University
issn 2633-4690
language English
publishDate 2022-01-01
publisher Wiley
record_format Article
series Advances in Pharmacological and Pharmaceutical Sciences
spelling doaj-art-d98507861c044d6d956f794e1e8157dd2025-02-03T00:59:36ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902022-01-01202210.1155/2022/1919469Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in MiceBeppe Galba Jean0Folefack Alice Irène1Atsamo Albert Donatien2Ngatanko Aïbassou Hervé-Hervé3Barga Mpoo Bertrand4Allah-Doum Nanou Gael5Nguedia Ymele Merline6Zemo Gamo Franklik7Dongmo Alain Bertrand8Dimo Théophile9Department of Biological ScienceDepartment of Biological ScienceDepartment of Animal Biology and PhysiologyDepartment of Biological ScienceDepartment of Biological ScienceDepartment of Biological ScienceDepartment of Biological ScienceDepartment of PsychologyDepartment of Animal Biology Faculty of Science University of DoualaDepartment of Animal Biology and PhysiologyThis study aimed to evaluate the neuroprotective effects of the ethanolic leaf extract of Crassocephalum crepidioides (Cc) on diazepam-induced amnesia in mice. Thirty mice distributed into six groups of five mice each were used. The normal control and negative control groups received 2% ethanol per os, the positive control group received piracetam (150 mg/kg, p.o), and three experimental groups were treated with three doses of ethanolic leaf extract of Cc (100, 200, and 400 mg/kg, p.o). All groups except the normal control group were co-treated with diazepam (3 mg/kg, i.p) daily for 14 days. The memory effects were evaluated using the Radial Arm Maze (RAM) and the Novel Object Recognition (NOR) tests, while the anti-depressive effects were evaluated using the tail suspension test. All animals were sacrificed at the end of the study. Hippocampi, isolated from the right hemisphere, were used to prepare a homogenate for the determination of oxidative stress biomarkers. The ethanolic leaf extract of cc significantly (p<0.001) decreased the number of working and reference memory errors in the RAM test and induced a significant (p<0.01) increase in the time spent exploring the novel object in the NOR test. The extract also induced a significant (p<0.001) increase in the mobility time in tail suspension. Moreover, compared to the negative control group, the extract significantly (p<0.01) increased superoxide dismutase activity and significantly (p<0.01) decreased malondialdehyde levels. The histopathological analysis of hippocampi showed that the cc extract increased cell density when compared with the negative control. These results suggest that the ethanolic left extract of cc could have neuroprotective properties, which could be attributed to its antioxidant properties.http://dx.doi.org/10.1155/2022/1919469
spellingShingle Beppe Galba Jean
Folefack Alice Irène
Atsamo Albert Donatien
Ngatanko Aïbassou Hervé-Hervé
Barga Mpoo Bertrand
Allah-Doum Nanou Gael
Nguedia Ymele Merline
Zemo Gamo Franklik
Dongmo Alain Bertrand
Dimo Théophile
Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
Advances in Pharmacological and Pharmaceutical Sciences
title Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
title_full Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
title_fullStr Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
title_full_unstemmed Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
title_short Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice
title_sort neuroprotective effects of the ethanolic leaf extract of crassocephalum crepidioides asteracaeae on diazepam induced amnesia in mice
url http://dx.doi.org/10.1155/2022/1919469
work_keys_str_mv AT beppegalbajean neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT folefackaliceirene neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT atsamoalbertdonatien neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT ngatankoaibassouherveherve neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT bargampoobertrand neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT allahdoumnanougael neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT nguediaymelemerline neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT zemogamofranklik neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT dongmoalainbertrand neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice
AT dimotheophile neuroprotectiveeffectsoftheethanolicleafextractofcrassocephalumcrepidioidesasteracaeaeondiazepaminducedamnesiainmice