Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
The objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydrox...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2016/1057260 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568035481747456 |
|---|---|
| author | Paulina-Guadalupe Miranda-Meléndez Gabriel-Alejandro Martínez-Castañón Nereyda Niño-Martínez Nuria Patiño-Marín Miguel-Ángel Casillas-Santana Brenda-Erendida Castillo-Silva Facundo Ruiz |
| author_facet | Paulina-Guadalupe Miranda-Meléndez Gabriel-Alejandro Martínez-Castañón Nereyda Niño-Martínez Nuria Patiño-Marín Miguel-Ángel Casillas-Santana Brenda-Erendida Castillo-Silva Facundo Ruiz |
| author_sort | Paulina-Guadalupe Miranda-Meléndez |
| collection | DOAJ |
| description | The objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydroxyapatite was doped with 3, 5, 10, and 20 wt% of europium. The obtained samples were characterized after they were dried at 80°C and hydrothermal treated at 120°C by 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and photoluminescence. Also, impregnation and release of 5-fluorouracil were assessed in PBS. The toxicity effects of all samples were studied using viability assays on human fibroblasts cells (HGF-1) in vitro. The sizes of the crystallites were about 10–70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9–0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5% of europium and loaded with 5-fluorouracil release almost 7 mg/L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours. |
| format | Article |
| id | doaj-art-d9828dfd78e948f787262b46c0d98579 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-d9828dfd78e948f787262b46c0d985792025-02-03T00:59:56ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2016-01-01201610.1155/2016/10572601057260Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped NanohydroxyapatitePaulina-Guadalupe Miranda-Meléndez0Gabriel-Alejandro Martínez-Castañón1Nereyda Niño-Martínez2Nuria Patiño-Marín3Miguel-Ángel Casillas-Santana4Brenda-Erendida Castillo-Silva5Facundo Ruiz6Doctorado Institucional en Ingeniería y Ciencia de los Materiales, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado Institucional en Ingeniería y Ciencia de los Materiales, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoThe objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydroxyapatite was doped with 3, 5, 10, and 20 wt% of europium. The obtained samples were characterized after they were dried at 80°C and hydrothermal treated at 120°C by 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and photoluminescence. Also, impregnation and release of 5-fluorouracil were assessed in PBS. The toxicity effects of all samples were studied using viability assays on human fibroblasts cells (HGF-1) in vitro. The sizes of the crystallites were about 10–70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9–0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5% of europium and loaded with 5-fluorouracil release almost 7 mg/L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours.http://dx.doi.org/10.1155/2016/1057260 |
| spellingShingle | Paulina-Guadalupe Miranda-Meléndez Gabriel-Alejandro Martínez-Castañón Nereyda Niño-Martínez Nuria Patiño-Marín Miguel-Ángel Casillas-Santana Brenda-Erendida Castillo-Silva Facundo Ruiz Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite Bioinorganic Chemistry and Applications |
| title | Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite |
| title_full | Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite |
| title_fullStr | Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite |
| title_full_unstemmed | Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite |
| title_short | Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite |
| title_sort | facile synthesis characterization and cytotoxic activity of europium doped nanohydroxyapatite |
| url | http://dx.doi.org/10.1155/2016/1057260 |
| work_keys_str_mv | AT paulinaguadalupemirandamelendez facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT gabrielalejandromartinezcastanon facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT nereydaninomartinez facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT nuriapatinomarin facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT miguelangelcasillassantana facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT brendaerendidacastillosilva facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite AT facundoruiz facilesynthesischaracterizationandcytotoxicactivityofeuropiumdopednanohydroxyapatite |