Characterization and functional analysis of chicken hnRNP protein
While the role of hnRNP proteins in modulating interferon signaling and virus replication in mammals has been extensively studied, the impact of chicken hnRNPs on innate immune response and Infectious Bursal Disease Virus (IBDV) replication remains largely unexplored. In this study, multiple chicken...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000963 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573251977478144 |
|---|---|
| author | Ke Wang Manzi Huang Qinghua Zeng Huansheng Wu |
| author_facet | Ke Wang Manzi Huang Qinghua Zeng Huansheng Wu |
| author_sort | Ke Wang |
| collection | DOAJ |
| description | While the role of hnRNP proteins in modulating interferon signaling and virus replication in mammals has been extensively studied, the impact of chicken hnRNPs on innate immune response and Infectious Bursal Disease Virus (IBDV) replication remains largely unexplored. In this study, multiple chicken hnRNPs were identified to interact with genomic dsRNA. Initially, these hnRNPs were found to be exclusively localized in the nucleus before IBDV infection. However, post-infection, they translocated into the cytoplasm to co-localize with the viral dsRNA. Furthermore, the effects of various chicken hnRNPs on IFN-β activation, induced by the chicken MDA5-MAVS-TBK1 and STING-IRF7 pathway, were analyzed. Among these hnRNPs, hnRNPA3 demonstrated the most significant reduction in inhibiting IFN-β activation at the MAVS step. Additionally, overexpression of chicken hnRNPA3 was found to markedly enhance IBDV replication. Conversely, silencing chicken hnRNPA3 via siRNA significantly increased IFN-β production, thereby substantially suppressing IBDV replication. In summary, our findings reveal for the first time that multiple chicken hnRNPs interact with viral dsRNA, modulate IFN-β production, and specifically, that chicken hnRNPA3 promotes IBDV replication. These insights into the role of chicken hnRNPs in viral replication and immune response could pave the way for new therapeutic strategies against IBDV. |
| format | Article |
| id | doaj-art-d9747057201f4afcab42f92166d071ba |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-d9747057201f4afcab42f92166d071ba2025-02-02T05:26:37ZengElsevierPoultry Science0032-57912025-03-011043104859Characterization and functional analysis of chicken hnRNP proteinKe Wang0Manzi Huang1Qinghua Zeng2Huansheng Wu3Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Zhimin Street, Qingshan Lake, Nanchang, 330045, PR China; Jiangxi Provincial Key laboratory for Animal Science and Technology, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR ChinaDepartment of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Zhimin Street, Qingshan Lake, Nanchang, 330045, PR China; Jiangxi Provincial Key laboratory for Animal Science and Technology, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR ChinaDepartment of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Zhimin Street, Qingshan Lake, Nanchang, 330045, PR China; Jiangxi Provincial Key laboratory for Animal Science and Technology, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR ChinaDepartment of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Zhimin Street, Qingshan Lake, Nanchang, 330045, PR China; Jiangxi Provincial Key laboratory for Animal Science and Technology, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, PR China; Correspondence author at Jiangxi Agricultural University, Zhimin Street, Qingshan Lake, Nanchang, 330045, PR ChinaWhile the role of hnRNP proteins in modulating interferon signaling and virus replication in mammals has been extensively studied, the impact of chicken hnRNPs on innate immune response and Infectious Bursal Disease Virus (IBDV) replication remains largely unexplored. In this study, multiple chicken hnRNPs were identified to interact with genomic dsRNA. Initially, these hnRNPs were found to be exclusively localized in the nucleus before IBDV infection. However, post-infection, they translocated into the cytoplasm to co-localize with the viral dsRNA. Furthermore, the effects of various chicken hnRNPs on IFN-β activation, induced by the chicken MDA5-MAVS-TBK1 and STING-IRF7 pathway, were analyzed. Among these hnRNPs, hnRNPA3 demonstrated the most significant reduction in inhibiting IFN-β activation at the MAVS step. Additionally, overexpression of chicken hnRNPA3 was found to markedly enhance IBDV replication. Conversely, silencing chicken hnRNPA3 via siRNA significantly increased IFN-β production, thereby substantially suppressing IBDV replication. In summary, our findings reveal for the first time that multiple chicken hnRNPs interact with viral dsRNA, modulate IFN-β production, and specifically, that chicken hnRNPA3 promotes IBDV replication. These insights into the role of chicken hnRNPs in viral replication and immune response could pave the way for new therapeutic strategies against IBDV.http://www.sciencedirect.com/science/article/pii/S0032579125000963hnRNPA3IBDVReplication |
| spellingShingle | Ke Wang Manzi Huang Qinghua Zeng Huansheng Wu Characterization and functional analysis of chicken hnRNP protein Poultry Science hnRNPA3 IBDV Replication |
| title | Characterization and functional analysis of chicken hnRNP protein |
| title_full | Characterization and functional analysis of chicken hnRNP protein |
| title_fullStr | Characterization and functional analysis of chicken hnRNP protein |
| title_full_unstemmed | Characterization and functional analysis of chicken hnRNP protein |
| title_short | Characterization and functional analysis of chicken hnRNP protein |
| title_sort | characterization and functional analysis of chicken hnrnp protein |
| topic | hnRNPA3 IBDV Replication |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000963 |
| work_keys_str_mv | AT kewang characterizationandfunctionalanalysisofchickenhnrnpprotein AT manzihuang characterizationandfunctionalanalysisofchickenhnrnpprotein AT qinghuazeng characterizationandfunctionalanalysisofchickenhnrnpprotein AT huanshengwu characterizationandfunctionalanalysisofchickenhnrnpprotein |