Inhibitory effect on the growth and aflatoxin biosynthesis of Aspergillus flavus by a strain of marine Bacillus
Using the method of filter paper diffusion, a strain of marine microorganism which exhibited highly inhibitory effect on Aspergillus flavus was screened. With the aid of 16S rDNA gene sequence, this marine strain was finally identified as a marine strain of Bacillus megaterium. Then, its inhibitory...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.04.006 |
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| Summary: | Using the method of filter paper diffusion, a strain of marine microorganism which exhibited highly inhibitory effect on Aspergillus flavus was screened. With the aid of 16S rDNA gene sequence, this marine strain was finally identified as a marine strain of Bacillus megaterium. Then, its inhibitory effects on mycelium extending, conidial germination and aflatoxin biosynthesis of A. flavus were further studied. Quantitative analysis kit for aflatoxins (Beacon) was used to determine the concentration of aflatoxin. The results showed that this marine strain exhibited good inhibition to the mycelium extending, conidial germination and aflatoxin biosynthesis of A. flavus. Eighty-seven percent conidia (1×10<sup>9</sup> CFU·mL<sup>-1</sup>B. megaterium) and 50.75% aflatoxin (1×10<sup>8</sup> CFU·mL<sup>-1</sup>B. megaterium) were inhibited, compared with the control group. The probable mechanism is accredited to some kinds of metabolites secreted by this marine strain which can inhibit the mycelium extending and conidial germination of A. flavus. |
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| ISSN: | 1008-9209 2097-5155 |