New food ingredient via acid-tolerant Rhizopus oligosporus growth

As the global population grows, the consumption of unsustainably produced proteins increases. Researching sustainable food sources with a positive environmental impact is essential. This research provides valuable insights into the production and application of nutrient rich food sources. The potent...

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Main Authors: A. Massa, M. Baiget, L.J. Rothschild, E. Axpe, C. Carrero-Carralero
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502224001938
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author A. Massa
M. Baiget
L.J. Rothschild
E. Axpe
C. Carrero-Carralero
author_facet A. Massa
M. Baiget
L.J. Rothschild
E. Axpe
C. Carrero-Carralero
author_sort A. Massa
collection DOAJ
description As the global population grows, the consumption of unsustainably produced proteins increases. Researching sustainable food sources with a positive environmental impact is essential. This research provides valuable insights into the production and application of nutrient rich food sources. The potential of the biomass generated through fermentation with Rhizophus oligosporus was evaluated as a new protein source with low environmental impact. A fermentation method has been optimized to maximize the biomass production of R. oligosporus mycelium in solid-state and submerged-state fermentation, yielding 5.9 g dry weight/L and 5.0 g dry weight/L, respectively. The growth medium for these fungi was adapted to acid-tolerant (pH 4 and 3) conditions to create a food development environment that is more difficult for pathogens to thrive in, both in solid state and submerged state fermentation. The highest protein content obtained was 38.4 % wet weight by solid state fermentation. Fungal biomass exhibited techno-functional properties relevant to food product development tested both on pH 4 and pH 3, showing high water absorption and emulsifying activity, particularly in submerged fermentation at pH 3. In contrast, solid-state fermentation at pH 4 resulted in higher emulsion stability. This research demonstrates the potential of R. oligosporus as an acid-tolerant, high-quality protein ingredient with valuable functional properties. Its efficient production using minimal resources makes it a valuable asset for the food industry.
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spelling doaj-art-d97109f2b8d64db89bd69d344ca852b42025-08-20T02:37:03ZengElsevierApplied Food Research2772-50222024-12-014210058310.1016/j.afres.2024.100583New food ingredient via acid-tolerant Rhizopus oligosporus growthA. Massa0M. Baiget1L.J. Rothschild2E. Axpe3C. Carrero-Carralero4Basque Culinary Center, Facultad de Ciencias Gastronómicas, Mondragon Unibertsitatea, Paseo Juan Avelino Barriola 101, Donostia-San Sebastián 20009, SpainBasque Culinary Center, Facultad de Ciencias Gastronómicas, Mondragon Unibertsitatea, Paseo Juan Avelino Barriola 101, Donostia-San Sebastián 20009, SpainNASA Ames Research Center, Moffett Field, CA 94035, USABasque Culinary Center, Facultad de Ciencias Gastronómicas, Mondragon Unibertsitatea, Paseo Juan Avelino Barriola 101, Donostia-San Sebastián 20009, SpainBasque Culinary Center, Facultad de Ciencias Gastronómicas, Mondragon Unibertsitatea, Paseo Juan Avelino Barriola 101, Donostia-San Sebastián 20009, Spain; Corresponding author.As the global population grows, the consumption of unsustainably produced proteins increases. Researching sustainable food sources with a positive environmental impact is essential. This research provides valuable insights into the production and application of nutrient rich food sources. The potential of the biomass generated through fermentation with Rhizophus oligosporus was evaluated as a new protein source with low environmental impact. A fermentation method has been optimized to maximize the biomass production of R. oligosporus mycelium in solid-state and submerged-state fermentation, yielding 5.9 g dry weight/L and 5.0 g dry weight/L, respectively. The growth medium for these fungi was adapted to acid-tolerant (pH 4 and 3) conditions to create a food development environment that is more difficult for pathogens to thrive in, both in solid state and submerged state fermentation. The highest protein content obtained was 38.4 % wet weight by solid state fermentation. Fungal biomass exhibited techno-functional properties relevant to food product development tested both on pH 4 and pH 3, showing high water absorption and emulsifying activity, particularly in submerged fermentation at pH 3. In contrast, solid-state fermentation at pH 4 resulted in higher emulsion stability. This research demonstrates the potential of R. oligosporus as an acid-tolerant, high-quality protein ingredient with valuable functional properties. Its efficient production using minimal resources makes it a valuable asset for the food industry.http://www.sciencedirect.com/science/article/pii/S2772502224001938Filamentous fungi biomassFungi proteinFood applicationSolid state fermentationSubmerged state fermentationRhizopus oligosporus
spellingShingle A. Massa
M. Baiget
L.J. Rothschild
E. Axpe
C. Carrero-Carralero
New food ingredient via acid-tolerant Rhizopus oligosporus growth
Applied Food Research
Filamentous fungi biomass
Fungi protein
Food application
Solid state fermentation
Submerged state fermentation
Rhizopus oligosporus
title New food ingredient via acid-tolerant Rhizopus oligosporus growth
title_full New food ingredient via acid-tolerant Rhizopus oligosporus growth
title_fullStr New food ingredient via acid-tolerant Rhizopus oligosporus growth
title_full_unstemmed New food ingredient via acid-tolerant Rhizopus oligosporus growth
title_short New food ingredient via acid-tolerant Rhizopus oligosporus growth
title_sort new food ingredient via acid tolerant rhizopus oligosporus growth
topic Filamentous fungi biomass
Fungi protein
Food application
Solid state fermentation
Submerged state fermentation
Rhizopus oligosporus
url http://www.sciencedirect.com/science/article/pii/S2772502224001938
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