Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression
Objective: Methyltransferase-like 3 (METTL3) plays a crucial role in cancer progression, both in m6A modification-dependent and −independent pathways. We aimed to elucidate the mechanism by which METTL3 and the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) contri...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Journal of Bone Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137425000363 |
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| author | Xiaohong Lu Yafei Li Ruie Li Jingheng Zhang Jiayu Peng Yan Zhang |
| author_facet | Xiaohong Lu Yafei Li Ruie Li Jingheng Zhang Jiayu Peng Yan Zhang |
| author_sort | Xiaohong Lu |
| collection | DOAJ |
| description | Objective: Methyltransferase-like 3 (METTL3) plays a crucial role in cancer progression, both in m6A modification-dependent and −independent pathways. We aimed to elucidate the mechanism by which METTL3 and the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) contribute to the pathogenesis of multiple myeloma (MM). Methods: Bone marrow samples were collected from 56 patients with MM and 42 healthy donors, followed by assessment of METTL3 and MALAT1 levels. An interaction between METTL3 and MALAT1 was also identified. METTL3- and MALAT1-related oligonucleotides were transfected into RPMI8226 and U266 cells to explore their role in cell growth. Apoptosis, migration, proliferation, and invasion of RPMI8226 and U266 cells were assayed. Results: Elevated METTL3 and MALAT1 levels were observed in patients with MM. Interference with METTL3 or MALAT1 inhibited the malignant behavior of RPMI8226 and U266 cells. There was an interaction between METTL3 and MALAT1. Overexpression of MALAT1 reversed the inhibitory effects of METTL3 interference on tumor cell malignancy. Conclusion: METTL3 augments MM development by enhancing MALAT1 expression. |
| format | Article |
| id | doaj-art-d954f5e94af04efb85acb1a9f8e23fd1 |
| institution | Kabale University |
| issn | 2212-1374 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj-art-d954f5e94af04efb85acb1a9f8e23fd12025-08-20T03:32:07ZengElsevierJournal of Bone Oncology2212-13742025-08-015310069510.1016/j.jbo.2025.100695Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progressionXiaohong Lu0Yafei Li1Ruie Li2Jingheng Zhang3Jiayu Peng4Yan Zhang5Department of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaDepartment of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaDepartment of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaDepartment of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaDepartment of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaCorresponding author at: Department of Oncology, Luzhou People’s Hospital, No. 316, Section 2, Jiu Gu Avenue, Jiangyang District, Luzhou 646000, Sichuan Province, China.; Department of Oncology, Luzhou People’s Hospital, Luzhou 646000 Sichuan Province, ChinaObjective: Methyltransferase-like 3 (METTL3) plays a crucial role in cancer progression, both in m6A modification-dependent and −independent pathways. We aimed to elucidate the mechanism by which METTL3 and the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) contribute to the pathogenesis of multiple myeloma (MM). Methods: Bone marrow samples were collected from 56 patients with MM and 42 healthy donors, followed by assessment of METTL3 and MALAT1 levels. An interaction between METTL3 and MALAT1 was also identified. METTL3- and MALAT1-related oligonucleotides were transfected into RPMI8226 and U266 cells to explore their role in cell growth. Apoptosis, migration, proliferation, and invasion of RPMI8226 and U266 cells were assayed. Results: Elevated METTL3 and MALAT1 levels were observed in patients with MM. Interference with METTL3 or MALAT1 inhibited the malignant behavior of RPMI8226 and U266 cells. There was an interaction between METTL3 and MALAT1. Overexpression of MALAT1 reversed the inhibitory effects of METTL3 interference on tumor cell malignancy. Conclusion: METTL3 augments MM development by enhancing MALAT1 expression.http://www.sciencedirect.com/science/article/pii/S2212137425000363Multiple myelomaMethyltransferase-like 3Long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1Tumor growthMigrationInvasion |
| spellingShingle | Xiaohong Lu Yafei Li Ruie Li Jingheng Zhang Jiayu Peng Yan Zhang Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression Journal of Bone Oncology Multiple myeloma Methyltransferase-like 3 Long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 Tumor growth Migration Invasion |
| title | Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression |
| title_full | Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression |
| title_fullStr | Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression |
| title_full_unstemmed | Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression |
| title_short | Regulatory role of the METTL3/MALAT1 axis in multiple myeloma progression |
| title_sort | regulatory role of the mettl3 malat1 axis in multiple myeloma progression |
| topic | Multiple myeloma Methyltransferase-like 3 Long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 Tumor growth Migration Invasion |
| url | http://www.sciencedirect.com/science/article/pii/S2212137425000363 |
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