Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens
The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits of broiler production. Recent research has shown that mulberry leaves and their extracts can be used to significantly improve the growth performance of livestock and poultry. The present study...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-09-01
|
| Series: | Animal Nutrition |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654524000520 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850209990183223296 |
|---|---|
| author | Zhenwu Huang Hongjian Dai Simeng Li Zhe Wang Quanwei Wei Zhonghua Ning Yuming Guo Fangxiong Shi Zengpeng Lv |
| author_facet | Zhenwu Huang Hongjian Dai Simeng Li Zhe Wang Quanwei Wei Zhonghua Ning Yuming Guo Fangxiong Shi Zengpeng Lv |
| author_sort | Zhenwu Huang |
| collection | DOAJ |
| description | The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits of broiler production. Recent research has shown that mulberry leaves and their extracts can be used to significantly improve the growth performance of livestock and poultry. The present study aims to elucidate the mechanisms involved in the regulation of skeletal muscle development in broiler offspring by dietary mulberry-leaf flavonoids (MLF) supplementation from the perspective of maternal effect theory. A total of 270 Qiling broiler breeder hens were randomly assigned to 3 treatments with different doses of MLF (0, 30, 60 mg/kg) for 8 weeks before collecting their fertilized eggs. The chicken offspring at 13 and 19 d of embryonic stage, and from 1 to 28 d old after hatching were included in this study. The results showed that maternal supplementation increased the breast muscle weight and body weight of the offspring at the embryo and chick stages (P < 0.05). This was followed by increased cross-sectional area of pectoral muscle fibres at 14 d (P < 0.05). Further determination revealed a tendency towards increased serum levels of insulin-like growth factor 1 (IGF-1) (P = 0.092) and muscle fibre count (P = 0.167) at 1 d post-hatching following maternal MLF treatment, while serum uric acid (UA) was decreased at 14 d after hatching (P < 0.05). Moreover, maternal MLF supplementation significantly up-regulated the mRNA expression of the myogenic regulatory factor Myf5 in skeletal muscle at the both embryonic and growth stages (P < 0.05). The relative abundance of the downstream protein of BMPR2, Smad1 and p-Smad1/5/9 in the TGFβ signalling pathway was significantly increased by maternal MLF treatment. Meanwhile, the increased expression of the target protein p-mTOR in the breast muscle of the offspring chicks is in accordance with the improved growth rate of the breast and the body. In conclusion, maternal MLF supplementation can promote muscle protein metabolism and muscle fibre development of chick embryos through upregulation of Myf5 expression and BMP/p-Smad1/5/9 axis, thereby improving growth performance of slow growing broiler. |
| format | Article |
| id | doaj-art-d938cb17011f4d06a2b0e1e4c2809fa6 |
| institution | OA Journals |
| issn | 2405-6545 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj-art-d938cb17011f4d06a2b0e1e4c2809fa62025-08-20T02:09:52ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452024-09-0118728310.1016/j.aninu.2024.04.005Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickensZhenwu Huang0Hongjian Dai1Simeng Li2Zhe Wang3Quanwei Wei4Zhonghua Ning5Yuming Guo6Fangxiong Shi7Zengpeng Lv8College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaSchool of Biotechnology, Aksu Vocational and Technical College, Aksu, 843000, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China; Corresponding author.The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits of broiler production. Recent research has shown that mulberry leaves and their extracts can be used to significantly improve the growth performance of livestock and poultry. The present study aims to elucidate the mechanisms involved in the regulation of skeletal muscle development in broiler offspring by dietary mulberry-leaf flavonoids (MLF) supplementation from the perspective of maternal effect theory. A total of 270 Qiling broiler breeder hens were randomly assigned to 3 treatments with different doses of MLF (0, 30, 60 mg/kg) for 8 weeks before collecting their fertilized eggs. The chicken offspring at 13 and 19 d of embryonic stage, and from 1 to 28 d old after hatching were included in this study. The results showed that maternal supplementation increased the breast muscle weight and body weight of the offspring at the embryo and chick stages (P < 0.05). This was followed by increased cross-sectional area of pectoral muscle fibres at 14 d (P < 0.05). Further determination revealed a tendency towards increased serum levels of insulin-like growth factor 1 (IGF-1) (P = 0.092) and muscle fibre count (P = 0.167) at 1 d post-hatching following maternal MLF treatment, while serum uric acid (UA) was decreased at 14 d after hatching (P < 0.05). Moreover, maternal MLF supplementation significantly up-regulated the mRNA expression of the myogenic regulatory factor Myf5 in skeletal muscle at the both embryonic and growth stages (P < 0.05). The relative abundance of the downstream protein of BMPR2, Smad1 and p-Smad1/5/9 in the TGFβ signalling pathway was significantly increased by maternal MLF treatment. Meanwhile, the increased expression of the target protein p-mTOR in the breast muscle of the offspring chicks is in accordance with the improved growth rate of the breast and the body. In conclusion, maternal MLF supplementation can promote muscle protein metabolism and muscle fibre development of chick embryos through upregulation of Myf5 expression and BMP/p-Smad1/5/9 axis, thereby improving growth performance of slow growing broiler.http://www.sciencedirect.com/science/article/pii/S2405654524000520Broiler breeder henMulberry-leaf flavonoidsOffspringProtein metabolismSkeletal muscle |
| spellingShingle | Zhenwu Huang Hongjian Dai Simeng Li Zhe Wang Quanwei Wei Zhonghua Ning Yuming Guo Fangxiong Shi Zengpeng Lv Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens Animal Nutrition Broiler breeder hen Mulberry-leaf flavonoids Offspring Protein metabolism Skeletal muscle |
| title | Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| title_full | Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| title_fullStr | Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| title_full_unstemmed | Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| title_short | Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| title_sort | maternal supplementation with mulberry leaf flavonoids improves the development of skeletal muscle in the offspring of chickens |
| topic | Broiler breeder hen Mulberry-leaf flavonoids Offspring Protein metabolism Skeletal muscle |
| url | http://www.sciencedirect.com/science/article/pii/S2405654524000520 |
| work_keys_str_mv | AT zhenwuhuang maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT hongjiandai maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT simengli maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT zhewang maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT quanweiwei maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT zhonghuaning maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT yumingguo maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT fangxiongshi maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens AT zengpenglv maternalsupplementationwithmulberryleafflavonoidsimprovesthedevelopmentofskeletalmuscleintheoffspringofchickens |