Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG
Simple and accurate analysis of cancer-related biomarkers is very important for disease screening and auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as a capture antibody and gold nanoparticle-conjugated goat anti-rabbit IgG as an enhanc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/15/8/612 |
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| author | Kang Yang Fang Yang Xiaoling Lu Hao Li Zeng Yang Qi Yin Lin Zhang You Long Chao Shen Liya Chen Bo Yao Chenghong Huang |
| author_facet | Kang Yang Fang Yang Xiaoling Lu Hao Li Zeng Yang Qi Yin Lin Zhang You Long Chao Shen Liya Chen Bo Yao Chenghong Huang |
| author_sort | Kang Yang |
| collection | DOAJ |
| description | Simple and accurate analysis of cancer-related biomarkers is very important for disease screening and auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as a capture antibody and gold nanoparticle-conjugated goat anti-rabbit IgG as an enhance antibody for the construction of a detection strategy for AFP analysis. Investigations indicated that the 50 nm diameter GNS-labeled capture antibody can specifically catch AFPs by direct detection profile or by further signal amplification through AuNP-tagged enhance antibody combination. Results showed that the developed method holds 8.6 ng/mL sensitivity, 20.0–110.0 ng/mL detection range, acceptable precision and fine accuracy, as well as favorable specificity. Results of application to real serum determination by the proposed method are highly related to those of the ECLIA method (correlation coefficient is 0.931). The proposed method has simple-operation merit and is very suitable for clinical screening of large-scale serum samples of cancers. |
| format | Article |
| id | doaj-art-d92bf24469314e8bb05dd24e8f2f6256 |
| institution | DOAJ |
| issn | 2079-4991 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-d92bf24469314e8bb05dd24e8f2f62562025-08-20T03:13:45ZengMDPI AGNanomaterials2079-49912025-04-0115861210.3390/nano15080612Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgGKang Yang0Fang Yang1Xiaoling Lu2Hao Li3Zeng Yang4Qi Yin5Lin Zhang6You Long7Chao Shen8Liya Chen9Bo Yao10Chenghong Huang11School of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science & Technology, Chongqing 401331, ChinaSimple and accurate analysis of cancer-related biomarkers is very important for disease screening and auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as a capture antibody and gold nanoparticle-conjugated goat anti-rabbit IgG as an enhance antibody for the construction of a detection strategy for AFP analysis. Investigations indicated that the 50 nm diameter GNS-labeled capture antibody can specifically catch AFPs by direct detection profile or by further signal amplification through AuNP-tagged enhance antibody combination. Results showed that the developed method holds 8.6 ng/mL sensitivity, 20.0–110.0 ng/mL detection range, acceptable precision and fine accuracy, as well as favorable specificity. Results of application to real serum determination by the proposed method are highly related to those of the ECLIA method (correlation coefficient is 0.931). The proposed method has simple-operation merit and is very suitable for clinical screening of large-scale serum samples of cancers.https://www.mdpi.com/2079-4991/15/8/612GNSsAuNPsimmunoassayAFP |
| spellingShingle | Kang Yang Fang Yang Xiaoling Lu Hao Li Zeng Yang Qi Yin Lin Zhang You Long Chao Shen Liya Chen Bo Yao Chenghong Huang Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG Nanomaterials GNSs AuNPs immunoassay AFP |
| title | Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG |
| title_full | Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG |
| title_fullStr | Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG |
| title_full_unstemmed | Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG |
| title_short | Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG |
| title_sort | facile immunoassay constructed by gold nanostar labeled rabbit afp antibody and gold nanoparticle conjugated goat anti rabbit igg |
| topic | GNSs AuNPs immunoassay AFP |
| url | https://www.mdpi.com/2079-4991/15/8/612 |
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