Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide

The diamondback moth (DBM), <i>Plutella xylostella,</i> is the main pest of Brassicas crops worldwide, and its recorded resistance to 101 active ingredients indicates it is difficult to control. The purpose of this study was to investigate the hypothesis that <i>P. xylostella</i...

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Main Authors: Natalia C. Bermúdez, Nataly de la Pava, Deividy V. Nascimento, Lilian M. S. Ribeiro, Herbert A. A. Siqueira, Jorge B. Torres
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Insects
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Online Access:https://www.mdpi.com/2075-4450/15/11/856
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author Natalia C. Bermúdez
Nataly de la Pava
Deividy V. Nascimento
Lilian M. S. Ribeiro
Herbert A. A. Siqueira
Jorge B. Torres
author_facet Natalia C. Bermúdez
Nataly de la Pava
Deividy V. Nascimento
Lilian M. S. Ribeiro
Herbert A. A. Siqueira
Jorge B. Torres
author_sort Natalia C. Bermúdez
collection DOAJ
description The diamondback moth (DBM), <i>Plutella xylostella,</i> is the main pest of Brassicas crops worldwide, and its recorded resistance to 101 active ingredients indicates it is difficult to control. The purpose of this study was to investigate the hypothesis that <i>P. xylostella</i> has fitness costs associated with its resistance to lufenuron, a chitin-synthesis inhibitor insecticide. Thus, concentration–mortality bioassays were performed for susceptible (REC-S), resistant (BZR-R) populations, their progenies F1 and F1′, and one established population without selection pressure (BZR-Rns) after four generations. A fertility life table was used to assess the biological performance of the REC-S and BZR-R. BZR-Rns of <i>P. xylostella</i>. The larval stage, longevity, and survival differed between populations. The reproductive rate (R<sub>0</sub>) was significantly lower in the F1 (♀R × ♂S) (28.19) and F1′ (♀S × ♂R) (34.06) progenies compared with their parents, but not with the relaxed BZR-Rns (39.39). The mean generation time (T), intrinsic rate of population growth (r<sub>m</sub>), and doubling time (DT) differed between REC-S and progenies, with fitness of 0.52 and 0.64 for F1 and F1′, respectively. Overall, the results suggest that the resistance of <i>P. xylostella</i> to lufenuron is stable and that low fitness costs appear to be associated with resistance to lufenuron, although heterozygotes showed lower fitness than their parents. Strategies such as preserving refuge areas, rotation of modes of action, etc., are essential for resistance management and prolonging the efficacy of control agents; this highlights the importance of integrated insecticide resistance management.
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spelling doaj-art-d924e18ce53d419facc7e2ff0bebf5ae2025-08-20T02:04:58ZengMDPI AGInsects2075-44502024-11-01151185610.3390/insects15110856Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor InsecticideNatalia C. Bermúdez0Nataly de la Pava1Deividy V. Nascimento2Lilian M. S. Ribeiro3Herbert A. A. Siqueira4Jorge B. Torres5Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois irmãos, 52171-900 Recife, PE, BrazilFacultad de Ingeniería Programa de Ingeniería Agronómica, Universidad del Magdalena, Calle 29H3 No 22-01, Santa Marta 470004, ColombiaDepartamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois irmãos, 52171-900 Recife, PE, BrazilDepartamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois irmãos, 52171-900 Recife, PE, BrazilDepartamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois irmãos, 52171-900 Recife, PE, BrazilDepartamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois irmãos, 52171-900 Recife, PE, BrazilThe diamondback moth (DBM), <i>Plutella xylostella,</i> is the main pest of Brassicas crops worldwide, and its recorded resistance to 101 active ingredients indicates it is difficult to control. The purpose of this study was to investigate the hypothesis that <i>P. xylostella</i> has fitness costs associated with its resistance to lufenuron, a chitin-synthesis inhibitor insecticide. Thus, concentration–mortality bioassays were performed for susceptible (REC-S), resistant (BZR-R) populations, their progenies F1 and F1′, and one established population without selection pressure (BZR-Rns) after four generations. A fertility life table was used to assess the biological performance of the REC-S and BZR-R. BZR-Rns of <i>P. xylostella</i>. The larval stage, longevity, and survival differed between populations. The reproductive rate (R<sub>0</sub>) was significantly lower in the F1 (♀R × ♂S) (28.19) and F1′ (♀S × ♂R) (34.06) progenies compared with their parents, but not with the relaxed BZR-Rns (39.39). The mean generation time (T), intrinsic rate of population growth (r<sub>m</sub>), and doubling time (DT) differed between REC-S and progenies, with fitness of 0.52 and 0.64 for F1 and F1′, respectively. Overall, the results suggest that the resistance of <i>P. xylostella</i> to lufenuron is stable and that low fitness costs appear to be associated with resistance to lufenuron, although heterozygotes showed lower fitness than their parents. Strategies such as preserving refuge areas, rotation of modes of action, etc., are essential for resistance management and prolonging the efficacy of control agents; this highlights the importance of integrated insecticide resistance management.https://www.mdpi.com/2075-4450/15/11/856diamondback mothlife tablebenzoylureasfitness costsdemography
spellingShingle Natalia C. Bermúdez
Nataly de la Pava
Deividy V. Nascimento
Lilian M. S. Ribeiro
Herbert A. A. Siqueira
Jorge B. Torres
Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
Insects
diamondback moth
life table
benzoylureas
fitness costs
demography
title Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
title_full Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
title_fullStr Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
title_full_unstemmed Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
title_short Fitness Costs in Diamondback Moth <i>Plutella xylostella</i> (L.) (Lepidoptera: Plutellidae) Resistant to Lufenuron, A Chitin-Synthesis Inhibitor Insecticide
title_sort fitness costs in diamondback moth i plutella xylostella i l lepidoptera plutellidae resistant to lufenuron a chitin synthesis inhibitor insecticide
topic diamondback moth
life table
benzoylureas
fitness costs
demography
url https://www.mdpi.com/2075-4450/15/11/856
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