Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections
Although the role of extracellular matrices in the development of glomerulosclerosis has been discussed widely, the cellular origin of type VI collagen in diabetic nephropathy (DN) has remained relatively unexplored. This study reports the distribution and cellular origin of type VI collagen in DN....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1997-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1997/280571 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560905320136704 |
|---|---|
| author | Mohammed Shawkat Razzaque Takehiko Koji Takashi Harada Takashi Taguchi |
| author_facet | Mohammed Shawkat Razzaque Takehiko Koji Takashi Harada Takashi Taguchi |
| author_sort | Mohammed Shawkat Razzaque |
| collection | DOAJ |
| description | Although the role of extracellular matrices in the development of glomerulosclerosis has been discussed widely, the cellular origin of type VI collagen in diabetic nephropathy (DN) has remained relatively unexplored. This study reports the distribution and cellular origin of type VI collagen in DN. Type VI collagen‐specific oligonucleotide probes and monoclonal antibody were used to assess the relative expression of mRNA for \alpha1 (VI) chain and its translated protein in paraffin‐embedded renal biopsy sections of DN. By immunohistochemistry, compared to the control, increased deposition of type VI collagen was noted in the diffuse and nodular lesions of diabetic glomeruli. For cellular localization of type VI collagen mRNA, paraffin‐embedded renal sections of the control and DN were hybridized in situ with digoxigenin (Dig)‐labeled antisense oligo‐DNA probe complementary to a part of \alpha1 (VI) mRNA. In comparison to the control kidney sections, increased numbers of intraglomerular cells (both mesangial and epithelial cells) were positive for α1 (VI) mRNA in renal biopsy sections of DN. From the results, we conclude that overexpression of type VI collagen by intraglomerular cells with its increased deposition might significantly contribute to the glomerulosclerosis found in DN. |
| format | Article |
| id | doaj-art-d917fe6003084d0e93bbe7946b656c3e |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-d917fe6003084d0e93bbe7946b656c3e2025-02-03T01:26:27ZengWileyAnalytical Cellular Pathology0921-89121878-36511997-01-0115317518110.1155/1997/280571Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy SectionsMohammed Shawkat Razzaque0Takehiko Koji1Takashi Harada2Takashi Taguchi3Second Department of Pathology, Nagasaki University School of Medicine, Nagasaki, JapanThird Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, JapanSecond Department of Medicine, Nagasaki University School of Medicine, Nagasaki, JapanSecond Department of Pathology, Nagasaki University School of Medicine, Nagasaki, JapanAlthough the role of extracellular matrices in the development of glomerulosclerosis has been discussed widely, the cellular origin of type VI collagen in diabetic nephropathy (DN) has remained relatively unexplored. This study reports the distribution and cellular origin of type VI collagen in DN. Type VI collagen‐specific oligonucleotide probes and monoclonal antibody were used to assess the relative expression of mRNA for \alpha1 (VI) chain and its translated protein in paraffin‐embedded renal biopsy sections of DN. By immunohistochemistry, compared to the control, increased deposition of type VI collagen was noted in the diffuse and nodular lesions of diabetic glomeruli. For cellular localization of type VI collagen mRNA, paraffin‐embedded renal sections of the control and DN were hybridized in situ with digoxigenin (Dig)‐labeled antisense oligo‐DNA probe complementary to a part of \alpha1 (VI) mRNA. In comparison to the control kidney sections, increased numbers of intraglomerular cells (both mesangial and epithelial cells) were positive for α1 (VI) mRNA in renal biopsy sections of DN. From the results, we conclude that overexpression of type VI collagen by intraglomerular cells with its increased deposition might significantly contribute to the glomerulosclerosis found in DN.http://dx.doi.org/10.1155/1997/280571 |
| spellingShingle | Mohammed Shawkat Razzaque Takehiko Koji Takashi Harada Takashi Taguchi Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections Analytical Cellular Pathology |
| title | Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections |
| title_full | Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections |
| title_fullStr | Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections |
| title_full_unstemmed | Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections |
| title_short | Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections |
| title_sort | identification of type vi collagen synthesizing cells in human diabetic glomerulosclerosis using renal biopsy sections |
| url | http://dx.doi.org/10.1155/1997/280571 |
| work_keys_str_mv | AT mohammedshawkatrazzaque identificationoftypevicollagensynthesizingcellsinhumandiabeticglomerulosclerosisusingrenalbiopsysections AT takehikokoji identificationoftypevicollagensynthesizingcellsinhumandiabeticglomerulosclerosisusingrenalbiopsysections AT takashiharada identificationoftypevicollagensynthesizingcellsinhumandiabeticglomerulosclerosisusingrenalbiopsysections AT takashitaguchi identificationoftypevicollagensynthesizingcellsinhumandiabeticglomerulosclerosisusingrenalbiopsysections |