Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice
Acute hepatic injury is a severe condition that is always accompanied by oxidative stress and inflammation, seriously threatening the health of the host. Probiotics have been shown to be involved in the regulation of antioxidant system and gut microbiota activity, but studies on the effects of yak d...
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| Format: | Article |
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550556/full |
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| author | Lei Wang Aoyun Li Xiaohu Zhang Mudassar Iqbal Zain Ul Aabdin Mengen Xu Quan Mo Jiakui Li Jiakui Li |
| author_facet | Lei Wang Aoyun Li Xiaohu Zhang Mudassar Iqbal Zain Ul Aabdin Mengen Xu Quan Mo Jiakui Li Jiakui Li |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Acute hepatic injury is a severe condition that is always accompanied by oxidative stress and inflammation, seriously threatening the health of the host. Probiotics have been shown to be involved in the regulation of antioxidant system and gut microbiota activity, but studies on the effects of yak derived Bacillus subtilis (B. subtilis) on acute liver injury and oxidative stress remain scarce. Here, we aim to explore the ameliorative effects of B. subtilis isolated from yaks on oxidative stress and hepatic injury caused by D-galactose, as well as the underlying processes. Results indicated that B. subtilis administration, particularly the BS3, significantly mitigated hepatic damage induced by D-galactose in mice as evidenced by ameliorating liver tissue damage as well as decreasing ALT (p < 0.05) and AST (p < 0.05) levels. Additionally, the B. subtilis intervention was demonstrated to enhance the antioxidant system in D-galactose-exposed mice, as manifested by increased T-AOC and SOD, alongside a decrease in MDA levels (p < 0.05). Meanwhile, B. subtilis intervention could effectively mitigate oxidative damage via modulating the Keap1/Nrf2 signaling pathway. Importantly, B. subtilis exhibited a pronounced protective effect against D-galactose-induced intestinal barrier dysfunction through improving tight junction proteins. The gut microbiota results suggest that BS3 alters the abundance of some gut flora such as Firmicutes phylum and Oscillibacter and Lachnospiraceae_NK4A136 genera, which affects the composition of the gut microbiota and reverses the decrease in the microbial richness index in mice. In summary, these findings demonstrated that B. subtilis isolated from yaks serve as a promising candidate to ameliorate oxidative damage and hepatic injury. Meanwhile, the positive regulation effect of B. subtilis on gut microbiota and intestinal mucosal barrier may be one of its underlying mechanisms to alleviate oxidative stress and hepatic injury. |
| format | Article |
| id | doaj-art-d91697a29eef4febbbb70a8a647413e6 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-d91697a29eef4febbbb70a8a647413e62025-08-20T02:02:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-03-011610.3389/fmicb.2025.15505561550556Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in miceLei Wang0Aoyun Li1Xiaohu Zhang2Mudassar Iqbal3Zain Ul Aabdin4Mengen Xu5Quan Mo6Jiakui Li7Jiakui Li8College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaDepartment of Preventive Veterinary Medicine and Public Health, Faculty of Veterinary and Animal Sciences, Ziauddin University, Karachi, PakistanCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Animal Science, Xizang Agricultural and Animal Husbandry University, Nyingchi, ChinaAcute hepatic injury is a severe condition that is always accompanied by oxidative stress and inflammation, seriously threatening the health of the host. Probiotics have been shown to be involved in the regulation of antioxidant system and gut microbiota activity, but studies on the effects of yak derived Bacillus subtilis (B. subtilis) on acute liver injury and oxidative stress remain scarce. Here, we aim to explore the ameliorative effects of B. subtilis isolated from yaks on oxidative stress and hepatic injury caused by D-galactose, as well as the underlying processes. Results indicated that B. subtilis administration, particularly the BS3, significantly mitigated hepatic damage induced by D-galactose in mice as evidenced by ameliorating liver tissue damage as well as decreasing ALT (p < 0.05) and AST (p < 0.05) levels. Additionally, the B. subtilis intervention was demonstrated to enhance the antioxidant system in D-galactose-exposed mice, as manifested by increased T-AOC and SOD, alongside a decrease in MDA levels (p < 0.05). Meanwhile, B. subtilis intervention could effectively mitigate oxidative damage via modulating the Keap1/Nrf2 signaling pathway. Importantly, B. subtilis exhibited a pronounced protective effect against D-galactose-induced intestinal barrier dysfunction through improving tight junction proteins. The gut microbiota results suggest that BS3 alters the abundance of some gut flora such as Firmicutes phylum and Oscillibacter and Lachnospiraceae_NK4A136 genera, which affects the composition of the gut microbiota and reverses the decrease in the microbial richness index in mice. In summary, these findings demonstrated that B. subtilis isolated from yaks serve as a promising candidate to ameliorate oxidative damage and hepatic injury. Meanwhile, the positive regulation effect of B. subtilis on gut microbiota and intestinal mucosal barrier may be one of its underlying mechanisms to alleviate oxidative stress and hepatic injury.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550556/fullyakBacillus subtilisoxidative stresshepatic injuryKeap1/Nrf2 signaling pathwaygut microbiota |
| spellingShingle | Lei Wang Aoyun Li Xiaohu Zhang Mudassar Iqbal Zain Ul Aabdin Mengen Xu Quan Mo Jiakui Li Jiakui Li Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice Frontiers in Microbiology yak Bacillus subtilis oxidative stress hepatic injury Keap1/Nrf2 signaling pathway gut microbiota |
| title | Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice |
| title_full | Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice |
| title_fullStr | Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice |
| title_full_unstemmed | Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice |
| title_short | Effect of Bacillus subtilis isolated from yaks on D-galactose-induced oxidative stress and hepatic damage in mice |
| title_sort | effect of bacillus subtilis isolated from yaks on d galactose induced oxidative stress and hepatic damage in mice |
| topic | yak Bacillus subtilis oxidative stress hepatic injury Keap1/Nrf2 signaling pathway gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550556/full |
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