AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity
The treatment of proctological conditions, including hemorrhoids, anal fissures, and perianal abscesses, is often complicated by bacterial infections, particularly those involving multidrug-resistant <i>Escherichia coli</i>. This study presents the synthesis, characterization, and biolog...
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MDPI AG
2025-05-01
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| author | Ewa Ciszkowicz Anna Miłoś Andrzej Łyskowski Justyna Buczkowicz Anna Nieczaj Katarzyna Lecka-Szlachta Konrad K. Hus Karol Sikora Damian Neubauer Marta Bauer Wojciech Kamysz Aleksandra Bocian |
| author_facet | Ewa Ciszkowicz Anna Miłoś Andrzej Łyskowski Justyna Buczkowicz Anna Nieczaj Katarzyna Lecka-Szlachta Konrad K. Hus Karol Sikora Damian Neubauer Marta Bauer Wojciech Kamysz Aleksandra Bocian |
| author_sort | Ewa Ciszkowicz |
| collection | DOAJ |
| description | The treatment of proctological conditions, including hemorrhoids, anal fissures, and perianal abscesses, is often complicated by bacterial infections, particularly those involving multidrug-resistant <i>Escherichia coli</i>. This study presents the synthesis, characterization, and biological evaluation of the newly designed synthetic peptide AMPEC4, inspired by cytotoxin 5 from <i>Naja ashei</i> snake venom. AMPEC4 demonstrated potent antimicrobial properties with MIC values of 100 and 200 µg/mL, effectively inhibiting biofilm formation (up to 84%) and eradicating the pre-formed biofilm by up to 35%. The antibacterial activity of AMPEC4 was further supported by a membrane permeabilization assay, demonstrating its capacity to disrupt bacterial membrane integrity in a dose-dependent manner. Furthermore, AMPEC4 significantly promoted fibroblast migration, a critical step in tissue regeneration, while exhibiting notable biocompatibility, as evidenced by the absence of hemolytic, cytotoxic, and genotoxic effects. By addressing both infection control and tissue regeneration, AMPEC4 represents a promising therapeutic strategy for managing chronic wounds, particularly in the challenging environment of the anorectal region. Its ability to target <i>Escherichia coli</i> reference and clinical strains while accelerating the wound-healing process underscores its potential for future clinical applications. |
| format | Article |
| id | doaj-art-d913490c64384beabd049f93372d6da5 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-d913490c64384beabd049f93372d6da52025-08-20T03:14:42ZengMDPI AGMolecules1420-30492025-05-013010216710.3390/molecules30102167AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial ActivityEwa Ciszkowicz0Anna Miłoś1Andrzej Łyskowski2Justyna Buczkowicz3Anna Nieczaj4Katarzyna Lecka-Szlachta5Konrad K. Hus6Karol Sikora7Damian Neubauer8Marta Bauer9Wojciech Kamysz10Aleksandra Bocian11Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDoctoral School of the Rzeszów University of Technology, al. Powstańców Warszawy 12, 35-959 Rzeszów, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, al. Gen. J. Hallera 107, 80-416 Gdańsk, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, al. Gen. J. Hallera 107, 80-416 Gdańsk, PolandDepartment of Analytical Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, al. Gen. J. Hallera 107, 80-416 Gdańsk, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, al. Gen. J. Hallera 107, 80-416 Gdańsk, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów, PolandThe treatment of proctological conditions, including hemorrhoids, anal fissures, and perianal abscesses, is often complicated by bacterial infections, particularly those involving multidrug-resistant <i>Escherichia coli</i>. This study presents the synthesis, characterization, and biological evaluation of the newly designed synthetic peptide AMPEC4, inspired by cytotoxin 5 from <i>Naja ashei</i> snake venom. AMPEC4 demonstrated potent antimicrobial properties with MIC values of 100 and 200 µg/mL, effectively inhibiting biofilm formation (up to 84%) and eradicating the pre-formed biofilm by up to 35%. The antibacterial activity of AMPEC4 was further supported by a membrane permeabilization assay, demonstrating its capacity to disrupt bacterial membrane integrity in a dose-dependent manner. Furthermore, AMPEC4 significantly promoted fibroblast migration, a critical step in tissue regeneration, while exhibiting notable biocompatibility, as evidenced by the absence of hemolytic, cytotoxic, and genotoxic effects. By addressing both infection control and tissue regeneration, AMPEC4 represents a promising therapeutic strategy for managing chronic wounds, particularly in the challenging environment of the anorectal region. Its ability to target <i>Escherichia coli</i> reference and clinical strains while accelerating the wound-healing process underscores its potential for future clinical applications.https://www.mdpi.com/1420-3049/30/10/2167antimicrobial peptideantibiofilm activity<i>Naja ashei</i><i>Escherichia coli</i>wound healingfibroblasts |
| spellingShingle | Ewa Ciszkowicz Anna Miłoś Andrzej Łyskowski Justyna Buczkowicz Anna Nieczaj Katarzyna Lecka-Szlachta Konrad K. Hus Karol Sikora Damian Neubauer Marta Bauer Wojciech Kamysz Aleksandra Bocian AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity Molecules antimicrobial peptide antibiofilm activity <i>Naja ashei</i> <i>Escherichia coli</i> wound healing fibroblasts |
| title | AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity |
| title_full | AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity |
| title_fullStr | AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity |
| title_full_unstemmed | AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity |
| title_short | AMPEC4: <i>Naja ashei</i> Venom-Derived Peptide as a Stimulator of Fibroblast Migration with Antibacterial Activity |
| title_sort | ampec4 i naja ashei i venom derived peptide as a stimulator of fibroblast migration with antibacterial activity |
| topic | antimicrobial peptide antibiofilm activity <i>Naja ashei</i> <i>Escherichia coli</i> wound healing fibroblasts |
| url | https://www.mdpi.com/1420-3049/30/10/2167 |
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