Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird

Sensorimotor computations for learning and behavior rely on precise patterns of synaptic connectivity. Yet, we typically lack the synaptic wiring diagrams for long-range connections between sensory and motor circuits in the brain. Here, we provide the synaptic wiring diagram for sensorimotor circuit...

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Main Authors: Massimo Trusel, Ziran Zhao, Danyal H Alam, Ethan S Marks, Maaya Z Ikeda, Todd F Roberts
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-06-01
Series:eLife
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Online Access:https://elifesciences.org/articles/104609
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author Massimo Trusel
Ziran Zhao
Danyal H Alam
Ethan S Marks
Maaya Z Ikeda
Todd F Roberts
author_facet Massimo Trusel
Ziran Zhao
Danyal H Alam
Ethan S Marks
Maaya Z Ikeda
Todd F Roberts
author_sort Massimo Trusel
collection DOAJ
description Sensorimotor computations for learning and behavior rely on precise patterns of synaptic connectivity. Yet, we typically lack the synaptic wiring diagrams for long-range connections between sensory and motor circuits in the brain. Here, we provide the synaptic wiring diagram for sensorimotor circuits involved in learning and production of male zebra finch song, a natural and ethologically relevant behavior. We examined the functional synaptic connectivity from the 4 main sensory afferent pathways onto the three known classes of projection neurons of the song premotor cortical region HVC. Recordings from hundreds of identified projection neurons reveal rules for monosynaptic connectivity and the existence of polysynaptic ensembles of excitatory and inhibitory neuronal populations in HVC. Circuit tracing further identifies novel connections between HVC’s presynaptic partners. Our results indicate a modular organization of ensemble-like networks for integrating long-range input with local circuits, providing important context for information flow and computations for learned vocal behavior.
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spelling doaj-art-d913250f7f4a45c79eebb73dda2bc0ef2025-08-20T03:23:51ZengeLife Sciences Publications LtdeLife2050-084X2025-06-011410.7554/eLife.104609Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbirdMassimo Trusel0https://orcid.org/0000-0001-6208-2476Ziran Zhao1Danyal H Alam2Ethan S Marks3Maaya Z Ikeda4Todd F Roberts5https://orcid.org/0000-0002-0967-6598Department of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, UT Southwestern Medical Center, Dallas, United StatesSensorimotor computations for learning and behavior rely on precise patterns of synaptic connectivity. Yet, we typically lack the synaptic wiring diagrams for long-range connections between sensory and motor circuits in the brain. Here, we provide the synaptic wiring diagram for sensorimotor circuits involved in learning and production of male zebra finch song, a natural and ethologically relevant behavior. We examined the functional synaptic connectivity from the 4 main sensory afferent pathways onto the three known classes of projection neurons of the song premotor cortical region HVC. Recordings from hundreds of identified projection neurons reveal rules for monosynaptic connectivity and the existence of polysynaptic ensembles of excitatory and inhibitory neuronal populations in HVC. Circuit tracing further identifies novel connections between HVC’s presynaptic partners. Our results indicate a modular organization of ensemble-like networks for integrating long-range input with local circuits, providing important context for information flow and computations for learned vocal behavior.https://elifesciences.org/articles/104609zebra finchsongbirdHVCoptogeneticsynaptic mappingvocal circuits
spellingShingle Massimo Trusel
Ziran Zhao
Danyal H Alam
Ethan S Marks
Maaya Z Ikeda
Todd F Roberts
Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
eLife
zebra finch
songbird
HVC
optogenetic
synaptic mapping
vocal circuits
title Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
title_full Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
title_fullStr Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
title_full_unstemmed Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
title_short Synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
title_sort synaptic connectivity of sensorimotor circuits for vocal imitation in the songbird
topic zebra finch
songbird
HVC
optogenetic
synaptic mapping
vocal circuits
url https://elifesciences.org/articles/104609
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AT ziranzhao synapticconnectivityofsensorimotorcircuitsforvocalimitationinthesongbird
AT danyalhalam synapticconnectivityofsensorimotorcircuitsforvocalimitationinthesongbird
AT ethansmarks synapticconnectivityofsensorimotorcircuitsforvocalimitationinthesongbird
AT maayazikeda synapticconnectivityofsensorimotorcircuitsforvocalimitationinthesongbird
AT toddfroberts synapticconnectivityofsensorimotorcircuitsforvocalimitationinthesongbird