Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions.
The network structure of the plant circadian clock is complex and direct regulatory interactions between individual components have proven particularly difficult to predict from genetic analyses. Here, we systematically investigate in vivo binding interactions between the morning-specific transcript...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0143943 |
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| author | Sally Adams Ian Manfield Peter Stockley Isabelle A Carré |
| author_facet | Sally Adams Ian Manfield Peter Stockley Isabelle A Carré |
| author_sort | Sally Adams |
| collection | DOAJ |
| description | The network structure of the plant circadian clock is complex and direct regulatory interactions between individual components have proven particularly difficult to predict from genetic analyses. Here, we systematically investigate in vivo binding interactions between the morning-specific transcription factor, LATE ELONGATED HYPOCOTYL (LHY) and the promoters of other components of the network. We then demonstrate the functionality of these interactions by testing the responsiveness of the target gene to an ethanol-induced change in expression level of the LHY protein. We uncover novel, negative autoregulatory feedback loops from LHY and the closely related CIRCADIAN CLOCK ASSOCIATED-1 (CCA1) onto their own and each other's expression. Furthermore we show that LHY acts as a repressor of all other clock components, including PSEUDO-RESPONSE REGULATORs (PRRs) 9 and 7, which were previously thought to be positive regulatory targets. These experimental results lead to a substantial revision of the morning loops of the clock. |
| format | Article |
| id | doaj-art-d9011ef5b5d24583b218fc10dddeec7c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d9011ef5b5d24583b218fc10dddeec7c2025-08-20T02:34:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014394310.1371/journal.pone.0143943Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions.Sally AdamsIan ManfieldPeter StockleyIsabelle A CarréThe network structure of the plant circadian clock is complex and direct regulatory interactions between individual components have proven particularly difficult to predict from genetic analyses. Here, we systematically investigate in vivo binding interactions between the morning-specific transcription factor, LATE ELONGATED HYPOCOTYL (LHY) and the promoters of other components of the network. We then demonstrate the functionality of these interactions by testing the responsiveness of the target gene to an ethanol-induced change in expression level of the LHY protein. We uncover novel, negative autoregulatory feedback loops from LHY and the closely related CIRCADIAN CLOCK ASSOCIATED-1 (CCA1) onto their own and each other's expression. Furthermore we show that LHY acts as a repressor of all other clock components, including PSEUDO-RESPONSE REGULATORs (PRRs) 9 and 7, which were previously thought to be positive regulatory targets. These experimental results lead to a substantial revision of the morning loops of the clock.https://doi.org/10.1371/journal.pone.0143943 |
| spellingShingle | Sally Adams Ian Manfield Peter Stockley Isabelle A Carré Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. PLoS ONE |
| title | Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. |
| title_full | Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. |
| title_fullStr | Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. |
| title_full_unstemmed | Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. |
| title_short | Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions. |
| title_sort | revised morning loops of the arabidopsis circadian clock based on analyses of direct regulatory interactions |
| url | https://doi.org/10.1371/journal.pone.0143943 |
| work_keys_str_mv | AT sallyadams revisedmorningloopsofthearabidopsiscircadianclockbasedonanalysesofdirectregulatoryinteractions AT ianmanfield revisedmorningloopsofthearabidopsiscircadianclockbasedonanalysesofdirectregulatoryinteractions AT peterstockley revisedmorningloopsofthearabidopsiscircadianclockbasedonanalysesofdirectregulatoryinteractions AT isabelleacarre revisedmorningloopsofthearabidopsiscircadianclockbasedonanalysesofdirectregulatoryinteractions |