miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages
Abstract It is still unclear the role of exosomes derived from fibro-adipogenic progenitors (FAP) on macrophage polarization and muscle repair. In this study, the effects of exosomes collected from primary FAPs isolated from acute injured muscles at different timepoints on macrophage polarization we...
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| Format: | Article |
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BMC
2025-07-01
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| Series: | Stem Cell Research & Therapy |
| Online Access: | https://doi.org/10.1186/s13287-025-04452-w |
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| author | Meng-meng Yang Jing-ya Luo Jia Cai Jin Qian Xiao-yan Zhu Hui-zhen Zou Gao-ming Li Jing Dai Huang Zhu Wei Li Li-dan Zhang Xia Kang |
| author_facet | Meng-meng Yang Jing-ya Luo Jia Cai Jin Qian Xiao-yan Zhu Hui-zhen Zou Gao-ming Li Jing Dai Huang Zhu Wei Li Li-dan Zhang Xia Kang |
| author_sort | Meng-meng Yang |
| collection | DOAJ |
| description | Abstract It is still unclear the role of exosomes derived from fibro-adipogenic progenitors (FAP) on macrophage polarization and muscle repair. In this study, the effects of exosomes collected from primary FAPs isolated from acute injured muscles at different timepoints on macrophage polarization were compared. We found exosomes derived from FAPs isolated at 10 (days post injury, DPI) can effectively induce M2 macrophage polarization both in vitro and in vivo. In addition, the pro-regenerative capacity of macrophages can be enhanced to support the activation of MuSCs and muscle repair. As we confirmed that RNAs played an important role in mediating the function of FAPD10-exos on inducing M2 macrophage polarization, using miRNA sequencing, miR-125b-5p was screened and confirmed as the key regulator to induce M2 macrophage polarization through targeting PTPN1. Furthermore, miR-125b-5p inhibited the activation of ERK pathway through inhibiting the expression of PTPN1. Overall, FAPD10-exos as well as miR-125b-5p exerted a nano-therapeutical function for muscle repair. |
| format | Article |
| id | doaj-art-d900c973a04a4cb1b91c1ed66dc088b5 |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-d900c973a04a4cb1b91c1ed66dc088b52025-08-20T03:45:57ZengBMCStem Cell Research & Therapy1757-65122025-07-0116111610.1186/s13287-025-04452-wmiRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophagesMeng-meng Yang0Jing-ya Luo1Jia Cai2Jin Qian3Xiao-yan Zhu4Hui-zhen Zou5Gao-ming Li6Jing Dai7Huang Zhu8Wei Li9Li-dan Zhang10Xia Kang11Center for Medical Epigenetics, School of Basic Medical Sciences, Chongqing Medical UniversityPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandLaboratory of Basic Medicine, The General Hospital of Western Theater CommandPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandLaboratory of Basic Medicine, The General Hospital of Western Theater CommandPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandDisease Surveillance Division, Center for Disease Control and Prevention of the Central Theater CommandPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandHyperbaric Oxygen Therapy Center, The General Hospital of Western Theater CommandPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandCenter for Medical Epigenetics, School of Basic Medical Sciences, Chongqing Medical UniversityPancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater CommandAbstract It is still unclear the role of exosomes derived from fibro-adipogenic progenitors (FAP) on macrophage polarization and muscle repair. In this study, the effects of exosomes collected from primary FAPs isolated from acute injured muscles at different timepoints on macrophage polarization were compared. We found exosomes derived from FAPs isolated at 10 (days post injury, DPI) can effectively induce M2 macrophage polarization both in vitro and in vivo. In addition, the pro-regenerative capacity of macrophages can be enhanced to support the activation of MuSCs and muscle repair. As we confirmed that RNAs played an important role in mediating the function of FAPD10-exos on inducing M2 macrophage polarization, using miRNA sequencing, miR-125b-5p was screened and confirmed as the key regulator to induce M2 macrophage polarization through targeting PTPN1. Furthermore, miR-125b-5p inhibited the activation of ERK pathway through inhibiting the expression of PTPN1. Overall, FAPD10-exos as well as miR-125b-5p exerted a nano-therapeutical function for muscle repair.https://doi.org/10.1186/s13287-025-04452-w |
| spellingShingle | Meng-meng Yang Jing-ya Luo Jia Cai Jin Qian Xiao-yan Zhu Hui-zhen Zou Gao-ming Li Jing Dai Huang Zhu Wei Li Li-dan Zhang Xia Kang miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages Stem Cell Research & Therapy |
| title | miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages |
| title_full | miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages |
| title_fullStr | miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages |
| title_full_unstemmed | miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages |
| title_short | miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages |
| title_sort | mirna 125b 5p rich exosomes derived from fibro adipogenic progenitors promoting muscle regeneration through inducing pro regenerative macrophages |
| url | https://doi.org/10.1186/s13287-025-04452-w |
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