The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration
Background: Degenerative aortic valve disease (DAVD) is a multifactorial process. We developed an animal model to analyze the isolated, local effect of reactive oxygen species (ROS) on its pathophysiology. Methods: We utilized a photodynamic reaction (PDR) as a source of ROS in the aortic valve by a...
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2024-12-01
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| author | Jessica Isabel Selig Yukiharu Sugimura Shintaro Katahira Marco Polidori Laura Alida Jacobi Olga Medovoj Sarah Betke Mareike Barth Artur Lichtenberg Payam Akhyari Jan-Philipp Minol |
| author_facet | Jessica Isabel Selig Yukiharu Sugimura Shintaro Katahira Marco Polidori Laura Alida Jacobi Olga Medovoj Sarah Betke Mareike Barth Artur Lichtenberg Payam Akhyari Jan-Philipp Minol |
| author_sort | Jessica Isabel Selig |
| collection | DOAJ |
| description | Background: Degenerative aortic valve disease (DAVD) is a multifactorial process. We developed an animal model to analyze the isolated, local effect of reactive oxygen species (ROS) on its pathophysiology. Methods: We utilized a photodynamic reaction (PDR) as a source of ROS in the aortic valve by aiming a laser at the aortic valve for 60 min after the administration of a photosensitizer 24 h prior. ROS, laser, and sham groups (<i>n</i> = 7 each) for every observation period (t = 0; t = 8 d; t = 84 d; t = 168 d) were established. The amount of ROS generation; morphological changes; inflammatory, immune, and apoptotic reactions; and hemodynamic changes in the aortic valves were assessed using appropriate histological, immunohistological, immunohistochemical, and echocardiographic methods. Results: The ROS group displayed an increased amount of ROS (<i>p</i> < 0.01) and increased inflammatory activation of the endothelium (<i>p</i> < 0.05) at t = 0. In the ROS group, aortic valves were calcified (<i>p</i> < 0.05) and the transvalvular gradient was increased (<i>p</i> < 0.01) at t = 168 d. Conclusion: The small animal model employed here may serve as a platform for analyzing ROS’s isolated role in the DAVD context. |
| format | Article |
| id | doaj-art-d9002d15afbc4952bf71debebb37bf0c |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-d9002d15afbc4952bf71debebb37bf0c2025-08-20T02:53:40ZengMDPI AGAntioxidants2076-39212024-12-011312157010.3390/antiox13121570The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve DegenerationJessica Isabel Selig0Yukiharu Sugimura1Shintaro Katahira2Marco Polidori3Laura Alida Jacobi4Olga Medovoj5Sarah Betke6Mareike Barth7Artur Lichtenberg8Payam Akhyari9Jan-Philipp Minol10Department of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyDepartment of Cardiac Surgery, University of Dusseldorf, Moorenstrasse 5, 40225 Dusseldorf, GermanyBackground: Degenerative aortic valve disease (DAVD) is a multifactorial process. We developed an animal model to analyze the isolated, local effect of reactive oxygen species (ROS) on its pathophysiology. Methods: We utilized a photodynamic reaction (PDR) as a source of ROS in the aortic valve by aiming a laser at the aortic valve for 60 min after the administration of a photosensitizer 24 h prior. ROS, laser, and sham groups (<i>n</i> = 7 each) for every observation period (t = 0; t = 8 d; t = 84 d; t = 168 d) were established. The amount of ROS generation; morphological changes; inflammatory, immune, and apoptotic reactions; and hemodynamic changes in the aortic valves were assessed using appropriate histological, immunohistological, immunohistochemical, and echocardiographic methods. Results: The ROS group displayed an increased amount of ROS (<i>p</i> < 0.01) and increased inflammatory activation of the endothelium (<i>p</i> < 0.05) at t = 0. In the ROS group, aortic valves were calcified (<i>p</i> < 0.05) and the transvalvular gradient was increased (<i>p</i> < 0.01) at t = 168 d. Conclusion: The small animal model employed here may serve as a platform for analyzing ROS’s isolated role in the DAVD context.https://www.mdpi.com/2076-3921/13/12/1570reactive oxygen speciesdegenerative aortic valve diseaseaortic valve stenosisphotodynamic reactionmTHCPtemoporfin |
| spellingShingle | Jessica Isabel Selig Yukiharu Sugimura Shintaro Katahira Marco Polidori Laura Alida Jacobi Olga Medovoj Sarah Betke Mareike Barth Artur Lichtenberg Payam Akhyari Jan-Philipp Minol The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration Antioxidants reactive oxygen species degenerative aortic valve disease aortic valve stenosis photodynamic reaction mTHCP temoporfin |
| title | The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration |
| title_full | The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration |
| title_fullStr | The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration |
| title_full_unstemmed | The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration |
| title_short | The Focal Induction of Reactive Oxygen Species in Rats as a Trigger of Aortic Valve Degeneration |
| title_sort | focal induction of reactive oxygen species in rats as a trigger of aortic valve degeneration |
| topic | reactive oxygen species degenerative aortic valve disease aortic valve stenosis photodynamic reaction mTHCP temoporfin |
| url | https://www.mdpi.com/2076-3921/13/12/1570 |
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