Kiwifruit sensitivity to boron: impact on physiological and molecular responses

Boron (B) is an essential micronutrient critical for crop growth and productivity. However, excessive boron concentrations can impair plant development, and detoxification remains a significant challenge. Understanding genetic variability and identifying tolerance mechanisms are crucial for developi...

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Main Authors: Gudeta Chalchisa, Abu Naim Md. Muzahid, Md Dulal Ali Mollah, Edmore Gasura, Xiaodong Xie, Xiaoying Liu, Haiyan Lv, Hua Tian, Caihong Zhong, Dawei Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1549854/full
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author Gudeta Chalchisa
Gudeta Chalchisa
Abu Naim Md. Muzahid
Abu Naim Md. Muzahid
Md Dulal Ali Mollah
Md Dulal Ali Mollah
Edmore Gasura
Xiaodong Xie
Xiaodong Xie
Xiaoying Liu
Xiaoying Liu
Haiyan Lv
Haiyan Lv
Hua Tian
Hua Tian
Caihong Zhong
Caihong Zhong
Dawei Li
Dawei Li
author_facet Gudeta Chalchisa
Gudeta Chalchisa
Abu Naim Md. Muzahid
Abu Naim Md. Muzahid
Md Dulal Ali Mollah
Md Dulal Ali Mollah
Edmore Gasura
Xiaodong Xie
Xiaodong Xie
Xiaoying Liu
Xiaoying Liu
Haiyan Lv
Haiyan Lv
Hua Tian
Hua Tian
Caihong Zhong
Caihong Zhong
Dawei Li
Dawei Li
author_sort Gudeta Chalchisa
collection DOAJ
description Boron (B) is an essential micronutrient critical for crop growth and productivity. However, excessive boron concentrations can impair plant development, and detoxification remains a significant challenge. Understanding genetic variability and identifying tolerance mechanisms are crucial for developing boron-resistant cultivars. This study explores the physiological and molecular responses of two Actinidia species, namely kiwifruit (A.chinensis) and kiwiberry (A.arguta), to varying levels of excess B. Under excessive B conditions, B accumulation followed the order roots< stems< leaves, with maximum concentrations of 68.6 mg/kg, 105 mg/kg, and 160.7 mg/kg in AC, and 68.2 mg/kg, 107 mg/kg, and 196.9 mg/kg in AA, respectively. B toxicity symptoms appeared in AA when B levels exceeded 50 mg/kg, leading to a 15–20% reduction in dry weight across roots, stems, and leaves. AC exhibited greater sensitivity, with a 20–30% reduction in dry biomass. Both species showed significant declines in chlorophyll a and b content under B stress, with alterations in the chlorophyll a/b ratio and increased oxidative stress. Additionally, stress-responsive genes, including 1-aminocyclopropane-1-carboxylate synthase (Actinidia10066) and xyloglucan endotransglucosylase/hydrolase (Actinidia11948), were downregulated in response to B stress, suggesting potential disruptions in growth and development. These findings provide valuable insights into the differential physiological and molecular responses to excess boron in Actinidia species, laying a foundation for functional genomics research and the development of boron-tolerant kiwifruit cultivars.
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spelling doaj-art-d8ec44248feb43f6b570e86926bea8902025-08-20T03:39:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-03-011610.3389/fpls.2025.15498541549854Kiwifruit sensitivity to boron: impact on physiological and molecular responsesGudeta Chalchisa0Gudeta Chalchisa1Abu Naim Md. Muzahid2Abu Naim Md. Muzahid3Md Dulal Ali Mollah4Md Dulal Ali Mollah5Edmore Gasura6Xiaodong Xie7Xiaodong Xie8Xiaoying Liu9Xiaoying Liu10Haiyan Lv11Haiyan Lv12Hua Tian13Hua Tian14Caihong Zhong15Caihong Zhong16Dawei Li17Dawei Li18CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaPlant Research Department, Gullele Botanical Garden, Addis Ababa, EthiopiaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaDepartment of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi, BangladeshDepartment of Plant Production Sciences and Technologies, University of Zimbabwe, Harare, ZimbabweCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Wuhan, ChinaBoron (B) is an essential micronutrient critical for crop growth and productivity. However, excessive boron concentrations can impair plant development, and detoxification remains a significant challenge. Understanding genetic variability and identifying tolerance mechanisms are crucial for developing boron-resistant cultivars. This study explores the physiological and molecular responses of two Actinidia species, namely kiwifruit (A.chinensis) and kiwiberry (A.arguta), to varying levels of excess B. Under excessive B conditions, B accumulation followed the order roots< stems< leaves, with maximum concentrations of 68.6 mg/kg, 105 mg/kg, and 160.7 mg/kg in AC, and 68.2 mg/kg, 107 mg/kg, and 196.9 mg/kg in AA, respectively. B toxicity symptoms appeared in AA when B levels exceeded 50 mg/kg, leading to a 15–20% reduction in dry weight across roots, stems, and leaves. AC exhibited greater sensitivity, with a 20–30% reduction in dry biomass. Both species showed significant declines in chlorophyll a and b content under B stress, with alterations in the chlorophyll a/b ratio and increased oxidative stress. Additionally, stress-responsive genes, including 1-aminocyclopropane-1-carboxylate synthase (Actinidia10066) and xyloglucan endotransglucosylase/hydrolase (Actinidia11948), were downregulated in response to B stress, suggesting potential disruptions in growth and development. These findings provide valuable insights into the differential physiological and molecular responses to excess boron in Actinidia species, laying a foundation for functional genomics research and the development of boron-tolerant kiwifruit cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2025.1549854/fullkiwifruitB functionboron stressphysiological responsegene regulation
spellingShingle Gudeta Chalchisa
Gudeta Chalchisa
Abu Naim Md. Muzahid
Abu Naim Md. Muzahid
Md Dulal Ali Mollah
Md Dulal Ali Mollah
Edmore Gasura
Xiaodong Xie
Xiaodong Xie
Xiaoying Liu
Xiaoying Liu
Haiyan Lv
Haiyan Lv
Hua Tian
Hua Tian
Caihong Zhong
Caihong Zhong
Dawei Li
Dawei Li
Kiwifruit sensitivity to boron: impact on physiological and molecular responses
Frontiers in Plant Science
kiwifruit
B function
boron stress
physiological response
gene regulation
title Kiwifruit sensitivity to boron: impact on physiological and molecular responses
title_full Kiwifruit sensitivity to boron: impact on physiological and molecular responses
title_fullStr Kiwifruit sensitivity to boron: impact on physiological and molecular responses
title_full_unstemmed Kiwifruit sensitivity to boron: impact on physiological and molecular responses
title_short Kiwifruit sensitivity to boron: impact on physiological and molecular responses
title_sort kiwifruit sensitivity to boron impact on physiological and molecular responses
topic kiwifruit
B function
boron stress
physiological response
gene regulation
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1549854/full
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