<i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience)
Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The improvement of MSC expansion standard protocols allowing receiving necessary MSC amount with the ability of self-re...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2022-11-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/756 |
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| author | T. V. Shamanskaya Ye. Yu. Osipova B. B. Purbueva A. Yu. Ustyugov T. A. Astrelina M. V. Yakovleva S. A. Rumyantsev |
| author_facet | T. V. Shamanskaya Ye. Yu. Osipova B. B. Purbueva A. Yu. Ustyugov T. A. Astrelina M. V. Yakovleva S. A. Rumyantsev |
| author_sort | T. V. Shamanskaya |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The improvement of MSC expansion standard protocols allowing receiving necessary MSC amount with the ability of self-renewal and multilineage differentiation potential for clinical application is required. The present article summarizes the current experience concerning uses of various culture mediums and supplements for the ex vivo MSC expansion. The author's experience of ex vivo MSC expansion using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of medium type on MSC growth and immunophenotype is analyzed. |
| format | Article |
| id | doaj-art-d8e6c42fd78a4abda57b62aac772dd09 |
| institution | Kabale University |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2022-11-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-d8e6c42fd78a4abda57b62aac772dd092025-08-20T04:00:14ZrusABV-pressОнкогематология1818-83462413-40232022-11-01036571634<i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience)T. V. Shamanskaya0Ye. Yu. Osipova1B. B. Purbueva2A. Yu. Ustyugov3T. A. Astrelina4M. V. Yakovleva5S. A. Rumyantsev6Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public healthFederal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public healthFederal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public healthFederal Clinical Research Center of Pediatric Hematology, Oncology and ImmunologyFederal Clinical Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Department of Public healthMoscow Department of Public healthFederal Clinical Research Center of Pediatric Hematology, Oncology and ImmunologyMesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The improvement of MSC expansion standard protocols allowing receiving necessary MSC amount with the ability of self-renewal and multilineage differentiation potential for clinical application is required. The present article summarizes the current experience concerning uses of various culture mediums and supplements for the ex vivo MSC expansion. The author's experience of ex vivo MSC expansion using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of medium type on MSC growth and immunophenotype is analyzed.https://oncohematology.abvpress.ru/ongm/article/view/756mesenchymal stem cellsexpansionculture medium |
| spellingShingle | T. V. Shamanskaya Ye. Yu. Osipova B. B. Purbueva A. Yu. Ustyugov T. A. Astrelina M. V. Yakovleva S. A. Rumyantsev <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) Онкогематология mesenchymal stem cells expansion culture medium |
| title | <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) |
| title_full | <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) |
| title_fullStr | <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) |
| title_full_unstemmed | <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) |
| title_short | <i>Ex vivo</i> expansion of mesenchymal stem cells in different culture conditions (the literature review and own experience) |
| title_sort | i ex vivo i expansion of mesenchymal stem cells in different culture conditions the literature review and own experience |
| topic | mesenchymal stem cells expansion culture medium |
| url | https://oncohematology.abvpress.ru/ongm/article/view/756 |
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