Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing
Amoxicillin (AMX) is commonly used antibiotic with a broad activity against Gram-negative and Gram-positive bacteria. Therefore, it is crucial access to an instant and real-time method for accurate and correct AMX determination. Here, we have developed a three-dimensional hydrogel modified with Euro...
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Elsevier
2025-06-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266605392400078X |
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| author | Kurdistan Fakhraldin Azeez Abdollah Salimi Hamed Mohtasham |
| author_facet | Kurdistan Fakhraldin Azeez Abdollah Salimi Hamed Mohtasham |
| author_sort | Kurdistan Fakhraldin Azeez |
| collection | DOAJ |
| description | Amoxicillin (AMX) is commonly used antibiotic with a broad activity against Gram-negative and Gram-positive bacteria. Therefore, it is crucial access to an instant and real-time method for accurate and correct AMX determination. Here, we have developed a three-dimensional hydrogel modified with Europium -Metal organic frameworks (Eu-MOFs) and carbon dots (CDs) as fluorophores, which allows for ratiometric fluorescence detection of AMX. The Eu-MOFs and CDs as an AMX probe trapped in Carboxymethyl cellulose (CMC) based hydrogel. In the presence of AMX, the fluorescence intensity of CDs@Eu-MOFs, enhanced at 448 nm and decreased at 568 nm under 360 nm excitation, due to energy transfer process. Under optimal conditions, AMX determined in the linear range of 10 μM to 106.7 μM, with a detection limit of 1.17 μM in liquid state. While, in the hydrogel state, the linear range for AMX detection is from 0.3 μM to 3.07 μM, with a detection limit of 0.08 μM. So, through applying hydrogel state, assay sensitivity is increased 32-times compared with conventional using liquid state. Furthermore, the change of the fluorescence color under UV irradiation also applied for colorimetric sensing of AMX at concentration up to 106 μM with detection limit 0.63 μM using smartphone RGB color sensing software. In addition, presented assay indicates strong selectivity for AMX over other biomolecules, salts and also other antibiotics. Also, it successfully detects different quantities of AMX in water and milk samples with excellent sensitivity, precision, and reliability in both liquid and hydrogel states. |
| format | Article |
| id | doaj-art-d8e47803cf8349a29af96ba07f99c835 |
| institution | OA Journals |
| issn | 2666-0539 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Sensors and Actuators Reports |
| spelling | doaj-art-d8e47803cf8349a29af96ba07f99c8352025-08-20T02:30:50ZengElsevierSensors and Actuators Reports2666-05392025-06-01910026210.1016/j.snr.2024.100262Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensingKurdistan Fakhraldin Azeez0Abdollah Salimi1Hamed Mohtasham2Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region, IraqDepartment of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran; Corresponding author.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, IranAmoxicillin (AMX) is commonly used antibiotic with a broad activity against Gram-negative and Gram-positive bacteria. Therefore, it is crucial access to an instant and real-time method for accurate and correct AMX determination. Here, we have developed a three-dimensional hydrogel modified with Europium -Metal organic frameworks (Eu-MOFs) and carbon dots (CDs) as fluorophores, which allows for ratiometric fluorescence detection of AMX. The Eu-MOFs and CDs as an AMX probe trapped in Carboxymethyl cellulose (CMC) based hydrogel. In the presence of AMX, the fluorescence intensity of CDs@Eu-MOFs, enhanced at 448 nm and decreased at 568 nm under 360 nm excitation, due to energy transfer process. Under optimal conditions, AMX determined in the linear range of 10 μM to 106.7 μM, with a detection limit of 1.17 μM in liquid state. While, in the hydrogel state, the linear range for AMX detection is from 0.3 μM to 3.07 μM, with a detection limit of 0.08 μM. So, through applying hydrogel state, assay sensitivity is increased 32-times compared with conventional using liquid state. Furthermore, the change of the fluorescence color under UV irradiation also applied for colorimetric sensing of AMX at concentration up to 106 μM with detection limit 0.63 μM using smartphone RGB color sensing software. In addition, presented assay indicates strong selectivity for AMX over other biomolecules, salts and also other antibiotics. Also, it successfully detects different quantities of AMX in water and milk samples with excellent sensitivity, precision, and reliability in both liquid and hydrogel states.http://www.sciencedirect.com/science/article/pii/S266605392400078XAmoxicillinEuropium-metal organic frameworksCarbon dotsRatiometric fluorescence assayHydrogelVisual sensing |
| spellingShingle | Kurdistan Fakhraldin Azeez Abdollah Salimi Hamed Mohtasham Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing Sensors and Actuators Reports Amoxicillin Europium-metal organic frameworks Carbon dots Ratiometric fluorescence assay Hydrogel Visual sensing |
| title | Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing |
| title_full | Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing |
| title_fullStr | Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing |
| title_full_unstemmed | Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing |
| title_short | Ratiometric fluorescence quantitation of amoxicillin based on CDs@Eu-MOFs incorporated 3D hydrogel using smartphone-assisted portable dual mode visual sensing |
| title_sort | ratiometric fluorescence quantitation of amoxicillin based on cds eu mofs incorporated 3d hydrogel using smartphone assisted portable dual mode visual sensing |
| topic | Amoxicillin Europium-metal organic frameworks Carbon dots Ratiometric fluorescence assay Hydrogel Visual sensing |
| url | http://www.sciencedirect.com/science/article/pii/S266605392400078X |
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