Multi-omics analysis of canine aging markers and evaluation of stem cell intervention
Abstract Dogs serve as ideal research subjects for aging studies. In this study, 9 aging-related cell populations are identified through single-cell RNA sequencing of dogs of different ages. Additionally, 9 CD8+ T cell senescence-specific markers conserved across species are identified. Furthermore,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-06-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08333-z |
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| author | Balun Li Yanheng Ding Miao Han Zengyu Zhang Zixi Ling Wenlong Zhu Xianjun Zhao Yihua Zhang Changrong Lv Na Li Mingzhi Liao Jinlian Hua |
| author_facet | Balun Li Yanheng Ding Miao Han Zengyu Zhang Zixi Ling Wenlong Zhu Xianjun Zhao Yihua Zhang Changrong Lv Na Li Mingzhi Liao Jinlian Hua |
| author_sort | Balun Li |
| collection | DOAJ |
| description | Abstract Dogs serve as ideal research subjects for aging studies. In this study, 9 aging-related cell populations are identified through single-cell RNA sequencing of dogs of different ages. Additionally, 9 CD8+ T cell senescence-specific markers conserved across species are identified. Furthermore, multi-omics technology is employed to characterize 17 transcriptional and protein markers, along with 5 metabolic markers, associated with stem cell aging. Penitrem A and UDP-N-acetylglucosamine are further validated as two consistent metabolic markers of both individual and cellular senescence. A customized metabolic assessment system and blood-based assessment framework specifically for aging dogs are developed. Notably, it is demonstrated that mesenchymal stem cells, particularly those overexpressing NMNAT1, can delay or reverse aging in dogs. This study sheds light on the mysteries of aging from multiple perspectives and provides a broad target for future research efforts aimed at uncovering the complexity of this fundamental biological process. |
| format | Article |
| id | doaj-art-d8e39e4c5352422d904cd58d734f6cd8 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-d8e39e4c5352422d904cd58d734f6cd82025-08-20T03:45:32ZengNature PortfolioCommunications Biology2399-36422025-06-018111810.1038/s42003-025-08333-zMulti-omics analysis of canine aging markers and evaluation of stem cell interventionBalun Li0Yanheng Ding1Miao Han2Zengyu Zhang3Zixi Ling4Wenlong Zhu5Xianjun Zhao6Yihua Zhang7Changrong Lv8Na Li9Mingzhi Liao10Jinlian Hua11College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Life Sciences, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityCollege of Life Sciences, Northwest A&F UniversityCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F UniversityAbstract Dogs serve as ideal research subjects for aging studies. In this study, 9 aging-related cell populations are identified through single-cell RNA sequencing of dogs of different ages. Additionally, 9 CD8+ T cell senescence-specific markers conserved across species are identified. Furthermore, multi-omics technology is employed to characterize 17 transcriptional and protein markers, along with 5 metabolic markers, associated with stem cell aging. Penitrem A and UDP-N-acetylglucosamine are further validated as two consistent metabolic markers of both individual and cellular senescence. A customized metabolic assessment system and blood-based assessment framework specifically for aging dogs are developed. Notably, it is demonstrated that mesenchymal stem cells, particularly those overexpressing NMNAT1, can delay or reverse aging in dogs. This study sheds light on the mysteries of aging from multiple perspectives and provides a broad target for future research efforts aimed at uncovering the complexity of this fundamental biological process.https://doi.org/10.1038/s42003-025-08333-z |
| spellingShingle | Balun Li Yanheng Ding Miao Han Zengyu Zhang Zixi Ling Wenlong Zhu Xianjun Zhao Yihua Zhang Changrong Lv Na Li Mingzhi Liao Jinlian Hua Multi-omics analysis of canine aging markers and evaluation of stem cell intervention Communications Biology |
| title | Multi-omics analysis of canine aging markers and evaluation of stem cell intervention |
| title_full | Multi-omics analysis of canine aging markers and evaluation of stem cell intervention |
| title_fullStr | Multi-omics analysis of canine aging markers and evaluation of stem cell intervention |
| title_full_unstemmed | Multi-omics analysis of canine aging markers and evaluation of stem cell intervention |
| title_short | Multi-omics analysis of canine aging markers and evaluation of stem cell intervention |
| title_sort | multi omics analysis of canine aging markers and evaluation of stem cell intervention |
| url | https://doi.org/10.1038/s42003-025-08333-z |
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