Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina

Retinal prostheses have been utilized in the treatment of blindness resulting from retinal degeneration. However, they have not met patient expectations, leading to market withdrawals. As a result, research continues to focus on improving visual perception, such as by modeling retinal neural activat...

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Main Authors: Marie Jung, Antje Willuweit, Viviana Rincón Montes
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Sensors
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Online Access:https://www.mdpi.com/1424-8220/25/12/3765
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author Marie Jung
Antje Willuweit
Viviana Rincón Montes
author_facet Marie Jung
Antje Willuweit
Viviana Rincón Montes
author_sort Marie Jung
collection DOAJ
description Retinal prostheses have been utilized in the treatment of blindness resulting from retinal degeneration. However, they have not met patient expectations, leading to market withdrawals. As a result, research continues to focus on improving visual perception, such as by modeling retinal neural activation. The retina’s electrical resistivity profile is key, as it influences the current spread during electrical stimulation. To advance efficient stimulation parameters, more data on the electrical properties of the retina in both its healthy and diseased state is needed. While this question has been addressed in mouse models, few data are available from rat models, whose bigger size is advantageous for many applications. To address this knowledge gap, we used flexible penetrating microelectrode arrays to measure intraretinal impedance and electrophysiological activity in retinas from both healthy (WT) and diseased RCS rats, an established model of retinal degeneration. Consequently, we calculated resistivity profiles, consistent with previous mouse retina findings, and correlated them with spontaneous spiking activity. Hence, both impedance and electrophysiological measurements across retinal depths are demonstrated as valuable tools to identify the optimal stimulation depth and simulate the electric field spread during electrical stimulation, which is particularly useful for the development of retinal prostheses. These findings demonstrate that resistivity changes in the degenerated retina significantly impact stimulation protocols and electric field propagation.
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spelling doaj-art-d8d2e2dff2a14b2ab7f0f6208d9003502025-08-20T03:29:52ZengMDPI AGSensors1424-82202025-06-012512376510.3390/s25123765Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat RetinaMarie Jung0Antje Willuweit1Viviana Rincón Montes2Institute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich, 52428 Jülich, GermanyInstitute of Neuroscience and Medicine (INM-4), Medical Imaging Physics, Forschungszentrum Jülich, Leo-Brandt-Str., 52428 Jülich, GermanyInstitute of Biological Information Processing (IBI-3), Bioelectronics, Forschungszentrum Jülich, 52428 Jülich, GermanyRetinal prostheses have been utilized in the treatment of blindness resulting from retinal degeneration. However, they have not met patient expectations, leading to market withdrawals. As a result, research continues to focus on improving visual perception, such as by modeling retinal neural activation. The retina’s electrical resistivity profile is key, as it influences the current spread during electrical stimulation. To advance efficient stimulation parameters, more data on the electrical properties of the retina in both its healthy and diseased state is needed. While this question has been addressed in mouse models, few data are available from rat models, whose bigger size is advantageous for many applications. To address this knowledge gap, we used flexible penetrating microelectrode arrays to measure intraretinal impedance and electrophysiological activity in retinas from both healthy (WT) and diseased RCS rats, an established model of retinal degeneration. Consequently, we calculated resistivity profiles, consistent with previous mouse retina findings, and correlated them with spontaneous spiking activity. Hence, both impedance and electrophysiological measurements across retinal depths are demonstrated as valuable tools to identify the optimal stimulation depth and simulate the electric field spread during electrical stimulation, which is particularly useful for the development of retinal prostheses. These findings demonstrate that resistivity changes in the degenerated retina significantly impact stimulation protocols and electric field propagation.https://www.mdpi.com/1424-8220/25/12/3765wildtype ratRoyal College of Surgeons ratretinal degenerationretinal impedanceelectrophysiology
spellingShingle Marie Jung
Antje Willuweit
Viviana Rincón Montes
Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
Sensors
wildtype rat
Royal College of Surgeons rat
retinal degeneration
retinal impedance
electrophysiology
title Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
title_full Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
title_fullStr Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
title_full_unstemmed Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
title_short Intraretinal Electrophysiology and Resistivity Profiles of WT and RCS Rat Retina
title_sort intraretinal electrophysiology and resistivity profiles of wt and rcs rat retina
topic wildtype rat
Royal College of Surgeons rat
retinal degeneration
retinal impedance
electrophysiology
url https://www.mdpi.com/1424-8220/25/12/3765
work_keys_str_mv AT mariejung intraretinalelectrophysiologyandresistivityprofilesofwtandrcsratretina
AT antjewilluweit intraretinalelectrophysiologyandresistivityprofilesofwtandrcsratretina
AT vivianarinconmontes intraretinalelectrophysiologyandresistivityprofilesofwtandrcsratretina