Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection
Gray leaf spot (GLS), caused by Cercospora zeae-maydis and Cercospora zeina, is a devastating foliar disease affecting maize production worldwide. However, the defense mechanisms underlying GLS resistance are poorly understood. A major quantitative trait locus (QTL), Qgls8, associated with GLS resis...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1580016/full |
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| author | Xiaomeng Sun Xiaomeng Sun Ruiyu Zhang Ruiyu Zhang Zongping Wang Zongping Wang Haotian Zhang Haotian Zhang Hairun Wen Hairun Wen Junli Zhang Naibin Yu Naibin Yu Zhe Wang Zhe Wang Huanhuan Tai Huanhuan Tai Qin Yang Qin Yang |
| author_facet | Xiaomeng Sun Xiaomeng Sun Ruiyu Zhang Ruiyu Zhang Zongping Wang Zongping Wang Haotian Zhang Haotian Zhang Hairun Wen Hairun Wen Junli Zhang Naibin Yu Naibin Yu Zhe Wang Zhe Wang Huanhuan Tai Huanhuan Tai Qin Yang Qin Yang |
| author_sort | Xiaomeng Sun |
| collection | DOAJ |
| description | Gray leaf spot (GLS), caused by Cercospora zeae-maydis and Cercospora zeina, is a devastating foliar disease affecting maize production worldwide. However, the defense mechanisms underlying GLS resistance are poorly understood. A major quantitative trait locus (QTL), Qgls8, associated with GLS resistance, was previously identified on maize chromosome 8 bin 8.06. Here we conducted transcriptome analyses on leaves from a pair of B73-teosinte near-isogenic lines (NILs) with contrasting Qgls8 alleles infected with C. zeina at 0, 4, 8, and 12 hours post inoculation (hpi). A total of 1225 up-regulated genes (URGs) were identified in the resistant line Qgls8-R compared with the susceptible line Qgls8-S across the four time points. By contrast, 908 URGs were identified in Qgls8-S. The URGs in Qgls8-R were significantly enriched in metabolic processes, phytohormone signaling, and response to biotic stress, while the URGs in Qgls8-S were mostly involved in plant growth and developmental processes. Additionally, C. zeina-induced URGs were consistently enriched in terpenoid metabolism and jasmonic acid (JA) signaling. Terpene- and JA-related genes showed increased expression at least at one time point after C. zeina infection which were confirmed by RT-qPCR. Furthermore, metabolite quantification indicated higher levels of JA and its isoleucine conjugate (JA-Ile) in Qgls8-R compared to Qgls8-S. Weighted gene co-expression network analysis (WGCNA) identified the module “turquoise”, which exhibited the highest positive correlation with Qgls8-R and was related to JA signaling. These findings suggest that the defense response mediated by terpenoid metabolism and the JA signaling pathway plays crucial roles in enhancing GLS resistance following C. zeina infection. |
| format | Article |
| id | doaj-art-d8d1420deebd4d94916a4f898c3a7cdf |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-d8d1420deebd4d94916a4f898c3a7cdf2025-08-20T03:24:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.15800161580016Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infectionXiaomeng Sun0Xiaomeng Sun1Ruiyu Zhang2Ruiyu Zhang3Zongping Wang4Zongping Wang5Haotian Zhang6Haotian Zhang7Hairun Wen8Hairun Wen9Junli Zhang10Naibin Yu11Naibin Yu12Zhe Wang13Zhe Wang14Huanhuan Tai15Huanhuan Tai16Qin Yang17Qin Yang18State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, ChinaHainan Research Institute of Northwest A&F University, Sanya, ChinaGray leaf spot (GLS), caused by Cercospora zeae-maydis and Cercospora zeina, is a devastating foliar disease affecting maize production worldwide. However, the defense mechanisms underlying GLS resistance are poorly understood. A major quantitative trait locus (QTL), Qgls8, associated with GLS resistance, was previously identified on maize chromosome 8 bin 8.06. Here we conducted transcriptome analyses on leaves from a pair of B73-teosinte near-isogenic lines (NILs) with contrasting Qgls8 alleles infected with C. zeina at 0, 4, 8, and 12 hours post inoculation (hpi). A total of 1225 up-regulated genes (URGs) were identified in the resistant line Qgls8-R compared with the susceptible line Qgls8-S across the four time points. By contrast, 908 URGs were identified in Qgls8-S. The URGs in Qgls8-R were significantly enriched in metabolic processes, phytohormone signaling, and response to biotic stress, while the URGs in Qgls8-S were mostly involved in plant growth and developmental processes. Additionally, C. zeina-induced URGs were consistently enriched in terpenoid metabolism and jasmonic acid (JA) signaling. Terpene- and JA-related genes showed increased expression at least at one time point after C. zeina infection which were confirmed by RT-qPCR. Furthermore, metabolite quantification indicated higher levels of JA and its isoleucine conjugate (JA-Ile) in Qgls8-R compared to Qgls8-S. Weighted gene co-expression network analysis (WGCNA) identified the module “turquoise”, which exhibited the highest positive correlation with Qgls8-R and was related to JA signaling. These findings suggest that the defense response mediated by terpenoid metabolism and the JA signaling pathway plays crucial roles in enhancing GLS resistance following C. zeina infection.https://www.frontiersin.org/articles/10.3389/fpls.2025.1580016/fullmaizegray leaf spottranscriptometerpenoid metabolismjasmonic acid |
| spellingShingle | Xiaomeng Sun Xiaomeng Sun Ruiyu Zhang Ruiyu Zhang Zongping Wang Zongping Wang Haotian Zhang Haotian Zhang Hairun Wen Hairun Wen Junli Zhang Naibin Yu Naibin Yu Zhe Wang Zhe Wang Huanhuan Tai Huanhuan Tai Qin Yang Qin Yang Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection Frontiers in Plant Science maize gray leaf spot transcriptome terpenoid metabolism jasmonic acid |
| title | Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection |
| title_full | Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection |
| title_fullStr | Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection |
| title_full_unstemmed | Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection |
| title_short | Comparative transcriptomics in B73-teosinte near-isogenic maize lines reveals key defense signaling and phytoalexins in response to Cercospora zeina infection |
| title_sort | comparative transcriptomics in b73 teosinte near isogenic maize lines reveals key defense signaling and phytoalexins in response to cercospora zeina infection |
| topic | maize gray leaf spot transcriptome terpenoid metabolism jasmonic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1580016/full |
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