Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury
Abstract Despite extensive research, spinal cord injuries (SCI), which could cause severe sensory, motor and autonomic dysfunction, remain largely incurable. Oligodendrocytes and oligodendrocyte precursor cells (ODC/OPC) play a crucial role in neural morphological repair and functional recovery foll...
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| Format: | Article |
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-90489-z |
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| author | Xi Yue Xunling Chen Yang Zang Jinliang Wu Guanhao Chen Hongyu Tan Kerong Yang |
| author_facet | Xi Yue Xunling Chen Yang Zang Jinliang Wu Guanhao Chen Hongyu Tan Kerong Yang |
| author_sort | Xi Yue |
| collection | DOAJ |
| description | Abstract Despite extensive research, spinal cord injuries (SCI), which could cause severe sensory, motor and autonomic dysfunction, remain largely incurable. Oligodendrocytes and oligodendrocyte precursor cells (ODC/OPC) play a crucial role in neural morphological repair and functional recovery following SCI. We performed single-cell sequencing (scRNA-seq) on 59,558 cells from 39 mouse samples, combined with microarray data from 164 SCI samples and 3 uninjured samples. We further validated our findings using a large clinical cohort consisting of 38 SCI patients, 10 healthy controls, and 10 trauma controls, assessed with the American Spinal Cord Injury Association (ASIA) scale. We proposed a novel SCI classification model based on the expression of prognostic differentially expressed ODC/OPC differentiation-related genes (PDEODGs). This model includes three types: Low ODC/OPC Score Classification (LOSC), Median ODC/OPC Score Classification (MOSC), and High ODC/OPC Score Classification (HOSC). Considering the relationship between these subtypes and prognosis, we speculated that enhancing ODC/OPC differentiation and inhibiting inflammatory infiltration may improve outcomes. Additionally, we identified potential treatments for SCI that target key genes within these subtypes, offering promising implications for therapy. |
| format | Article |
| id | doaj-art-d8ceeb824fb94a99b1ef18a636a6203c |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-d8ceeb824fb94a99b1ef18a636a6203c2025-08-20T02:15:00ZengNature PortfolioScientific Reports2045-23222025-02-0115111710.1038/s41598-025-90489-zBioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord InjuryXi Yue0Xunling Chen1Yang Zang2Jinliang Wu3Guanhao Chen4Hongyu Tan5Kerong Yang6Department of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityChildren’s Hospital Affiliated of Zhengzhou UniversityDepartment of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityDepartment of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityDepartment of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityDepartment of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityDepartment of Orthopedics, First Affiliated Hospital of Zhengzhou UniversityAbstract Despite extensive research, spinal cord injuries (SCI), which could cause severe sensory, motor and autonomic dysfunction, remain largely incurable. Oligodendrocytes and oligodendrocyte precursor cells (ODC/OPC) play a crucial role in neural morphological repair and functional recovery following SCI. We performed single-cell sequencing (scRNA-seq) on 59,558 cells from 39 mouse samples, combined with microarray data from 164 SCI samples and 3 uninjured samples. We further validated our findings using a large clinical cohort consisting of 38 SCI patients, 10 healthy controls, and 10 trauma controls, assessed with the American Spinal Cord Injury Association (ASIA) scale. We proposed a novel SCI classification model based on the expression of prognostic differentially expressed ODC/OPC differentiation-related genes (PDEODGs). This model includes three types: Low ODC/OPC Score Classification (LOSC), Median ODC/OPC Score Classification (MOSC), and High ODC/OPC Score Classification (HOSC). Considering the relationship between these subtypes and prognosis, we speculated that enhancing ODC/OPC differentiation and inhibiting inflammatory infiltration may improve outcomes. Additionally, we identified potential treatments for SCI that target key genes within these subtypes, offering promising implications for therapy.https://doi.org/10.1038/s41598-025-90489-zSpinal cord injuryODC/OPCMulti-omicsRegulatory networkPotential drugs |
| spellingShingle | Xi Yue Xunling Chen Yang Zang Jinliang Wu Guanhao Chen Hongyu Tan Kerong Yang Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury Scientific Reports Spinal cord injury ODC/OPC Multi-omics Regulatory network Potential drugs |
| title | Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury |
| title_full | Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury |
| title_fullStr | Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury |
| title_full_unstemmed | Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury |
| title_short | Bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord Injury |
| title_sort | bioinformatics analysis reveals key mechanisms of oligodendrocytes and oligodendrocyte precursor cells regulation in spinal cord injury |
| topic | Spinal cord injury ODC/OPC Multi-omics Regulatory network Potential drugs |
| url | https://doi.org/10.1038/s41598-025-90489-z |
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