Researches on new and simple test for elifferentiation of tuberculosis anel brucellosis
This research was carried out on a total of 50 ili animals suspected from tuberculosis and brucellosis which were brought to the clinics of Veterinary Faculty and on 10 healthy cattle that have being raised at o ur experimental farm.<p> All of the animals were inspected clinically and the new...
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=950 |
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| Summary: | This research was carried out on a total of 50 ili animals
suspected from tuberculosis and brucellosis which were brought to the
clinics of Veterinary Faculty and on 10 healthy cattle that have being
raised at o ur experimental farm.<p>
All of the animals were inspected clinically and the new and simple
test ,Balcı test was applied to those ones with tuberculosis and brusellosis.
Based on the test evaluation, tuberculin was given in the case positive
reaction and the serological methods were applied in the brusellosis
incidents.<p>
The Balcı and tuberculin tests were both positive at 25 samples out
of 40, the Balcı test was positive, but the tuberculin test was negative
for 5 samples; on the other hand the Balcı and tuberculin were both
negative for 10 samples.<p>
The same test was applied to 8 cattle and 2 rams with bruceilasis and
a positive relation was obtained. This was affirmed by the Iabaratarial
findings.<p>
Methodology of Balcı test :<br>
A. Solutions<br>
1 - 0.75 % Eosin Solution (Solution A). This solution was obtained
by dissolving Eosin in distilled water.<p>
2 - Glycine - saline Buffer solution (pH 8.2), obtained by dissolving
7.5 gr glycine in 977 cc distilled water adding 2.5 cc 1 N Na OH the
mixture was levelled to 1000 cc by adding distilled water and furthermore
8.5 gr NaCL was added.<p>
3 - 1 % CaCL<sub>2</sub> solution, obtained by dissolving 1 gr granul ated
pure CaCI<sub>2</sub> in 100 cc distiled water.<p>
4 - Three parts of Glycine - Saline Buffer Solution were mixed
with one part of 1% CaCI<sub>2</sub> solution. Sodium Acide of 1/20 concentration
in distiled water was added to this mixture until the mixture reached a
sodium acide concentration of 1/2000. The resulting mixture was used in
the test and it is called solution B.<p>
B. Aplication<p>
A full drop of blood obtained from the top of ear was placed on a
slide. A drop of solution was added and mixed with the tip of the lanset
and the mixture was spread over the slide. Next a drop of solution B was
added, mixed and spread smilarly. The slide was rolled back and forth
a couple of times. When many red granulations appeared in the mixture
within two minutes the test was considered as positive. |
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| ISSN: | 1309-6958 2146-1953 |