Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration

Brucellosis is an important zoonotic infection of Pakistani ruminants. Muzaffargarh is an historically and agriculturally important district of Pakistan and the data on brucellosis remains scarce in this district. To overcome this gap, a preliminary study was performed to estimate the occurrence of...

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Main Authors: Sami Ullah, Tariq Jamil, Muhammad Asif, Waqas Ahmad, Heinrich Neubauer
Format: Article
Language:English
Published: German Multidisciplinary Publishing Center (GMPC) 2022-01-01
Series:German Journal of Veterinary Research
Online Access:https://doi.org/10.51585/gjvr.2022.1.0039
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author Sami Ullah
Tariq Jamil
Muhammad Asif
Waqas Ahmad
Heinrich Neubauer
author_facet Sami Ullah
Tariq Jamil
Muhammad Asif
Waqas Ahmad
Heinrich Neubauer
author_sort Sami Ullah
collection DOAJ
description Brucellosis is an important zoonotic infection of Pakistani ruminants. Muzaffargarh is an historically and agriculturally important district of Pakistan and the data on brucellosis remains scarce in this district. To overcome this gap, a preliminary study was performed to estimate the occurrence of brucellosis. A total of 53 sera were screened by Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) followed by a Brucella genus specific conventional Polymerase Chain Reaction (PCR) and gel visualization of the PCR products. Four (7.54%) samples were positive by RBPT and SAT and only 1 (1.88%) sample was positive by PCR. The results point towards a persistent zoonotic threat in cattle in the district and the potential spill over infection when close contact to other animal species. Pasteurization of milk and regular screening of animals is recommended to prevent transmission.
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institution Kabale University
issn 2703-1322
language English
publishDate 2022-01-01
publisher German Multidisciplinary Publishing Center (GMPC)
record_format Article
series German Journal of Veterinary Research
spelling doaj-art-d8c684f205524d1d8d33ddccfb643f0f2025-08-20T03:24:35ZengGerman Multidisciplinary Publishing Center (GMPC)German Journal of Veterinary Research2703-13222022-01-0113510.51585/gjvr.2022.1.0039Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaborationSami UllahTariq JamilMuhammad AsifWaqas AhmadHeinrich NeubauerBrucellosis is an important zoonotic infection of Pakistani ruminants. Muzaffargarh is an historically and agriculturally important district of Pakistan and the data on brucellosis remains scarce in this district. To overcome this gap, a preliminary study was performed to estimate the occurrence of brucellosis. A total of 53 sera were screened by Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) followed by a Brucella genus specific conventional Polymerase Chain Reaction (PCR) and gel visualization of the PCR products. Four (7.54%) samples were positive by RBPT and SAT and only 1 (1.88%) sample was positive by PCR. The results point towards a persistent zoonotic threat in cattle in the district and the potential spill over infection when close contact to other animal species. Pasteurization of milk and regular screening of animals is recommended to prevent transmission.https://doi.org/10.51585/gjvr.2022.1.0039
spellingShingle Sami Ullah
Tariq Jamil
Muhammad Asif
Waqas Ahmad
Heinrich Neubauer
Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
German Journal of Veterinary Research
title Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
title_full Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
title_fullStr Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
title_full_unstemmed Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
title_short Brucellosis remains a neglected disease in District Muzaffargarh of Pakistani Punjab: A call for multidisciplinary collaboration
title_sort brucellosis remains a neglected disease in district muzaffargarh of pakistani punjab a call for multidisciplinary collaboration
url https://doi.org/10.51585/gjvr.2022.1.0039
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