Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience

The gold standard for histological acute cellular rejection diagnosis is transbronchial forceps biopsy (FB), but in recent years, transbronchial cryobiopsy (CB) has been increasingly used. This study aims to compare the diagnostic rate and safety of FBs and CBs performed in two different periods. We...

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Main Authors: Davide Tosi, Margherita Brivio, Sara Franzi, Alessandro Palleschi, Gianluca Bonitta, Gianluca Lopez, Ilaria Righi, Paolo Mendogni, Margherita Cattaneo, Francesco Damarco, Letizia Morlacchi, Valeria Rossetti, Lorenzo Rosso
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Language:English
Published: MDPI AG 2024-11-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/14/11/1474
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author Davide Tosi
Margherita Brivio
Sara Franzi
Alessandro Palleschi
Gianluca Bonitta
Gianluca Lopez
Ilaria Righi
Paolo Mendogni
Margherita Cattaneo
Francesco Damarco
Letizia Morlacchi
Valeria Rossetti
Lorenzo Rosso
author_facet Davide Tosi
Margherita Brivio
Sara Franzi
Alessandro Palleschi
Gianluca Bonitta
Gianluca Lopez
Ilaria Righi
Paolo Mendogni
Margherita Cattaneo
Francesco Damarco
Letizia Morlacchi
Valeria Rossetti
Lorenzo Rosso
author_sort Davide Tosi
collection DOAJ
description The gold standard for histological acute cellular rejection diagnosis is transbronchial forceps biopsy (FB), but in recent years, transbronchial cryobiopsy (CB) has been increasingly used. This study aims to compare the diagnostic rate and safety of FBs and CBs performed in two different periods. We retrospectively reviewed our case history for the two biopsy procedures: 251 FBs (223 for surveillance purposes and 28 for clinical indication) and 218 consecutive CBs (159 for surveillance purposes and 59 for clinical indication). All biopsies were scored according to the ISHLT criteria. Diagnostic yield was higher in the CB group for all the parameters considered: a grade of acute rejection (AR) was detected in 95.0% vs. 84.5% in the CB vs. FB groups (<i>p</i> < 0.001). The diagnostic rate of airway inflammation was 65.1% vs. 51.8% (<i>p</i> = 0.005), and 89.0% vs. 64.9% (<i>p</i> < 0.001) for chronic rejection. Pneumothorax requiring chest drainage occurred in 4% of the CB group and 3% of the FB group. Moderate and severe bleeding complicated CB and FB procedures in seven (3%) and three cases (1%), respectively. Transbronchial cryobiopsies improved the diagnostic yield in the monitoring of the lung allograft. The complication rate did not increase significantly in CBs vs. FBs.
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spelling doaj-art-d8bf21b8f59a4f03b5f8ec90302f1e0f2025-08-20T02:05:03ZengMDPI AGLife2075-17292024-11-011411147410.3390/life14111474Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre ExperienceDavide Tosi0Margherita Brivio1Sara Franzi2Alessandro Palleschi3Gianluca Bonitta4Gianluca Lopez5Ilaria Righi6Paolo Mendogni7Margherita Cattaneo8Francesco Damarco9Letizia Morlacchi10Valeria Rossetti11Lorenzo Rosso12Thoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyDepartment of Pathophysiology and Transplantation, University of Milan, Via Francesco Sforza 12, 20122 Milan, ItalyDivision of Pathology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyDepartment of Pathophysiology and Transplantation, University of Milan, Via Francesco Sforza 12, 20122 Milan, ItalyRespiratory Unit and Cystic Fibrosis Adult Center, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, ItalyThe gold standard for histological acute cellular rejection diagnosis is transbronchial forceps biopsy (FB), but in recent years, transbronchial cryobiopsy (CB) has been increasingly used. This study aims to compare the diagnostic rate and safety of FBs and CBs performed in two different periods. We retrospectively reviewed our case history for the two biopsy procedures: 251 FBs (223 for surveillance purposes and 28 for clinical indication) and 218 consecutive CBs (159 for surveillance purposes and 59 for clinical indication). All biopsies were scored according to the ISHLT criteria. Diagnostic yield was higher in the CB group for all the parameters considered: a grade of acute rejection (AR) was detected in 95.0% vs. 84.5% in the CB vs. FB groups (<i>p</i> < 0.001). The diagnostic rate of airway inflammation was 65.1% vs. 51.8% (<i>p</i> = 0.005), and 89.0% vs. 64.9% (<i>p</i> < 0.001) for chronic rejection. Pneumothorax requiring chest drainage occurred in 4% of the CB group and 3% of the FB group. Moderate and severe bleeding complicated CB and FB procedures in seven (3%) and three cases (1%), respectively. Transbronchial cryobiopsies improved the diagnostic yield in the monitoring of the lung allograft. The complication rate did not increase significantly in CBs vs. FBs.https://www.mdpi.com/2075-1729/14/11/1474lung transplantationacute cellular rejectionchronic rejectionendoscopic surveillancetransbronchial biopsycryobiopsy
spellingShingle Davide Tosi
Margherita Brivio
Sara Franzi
Alessandro Palleschi
Gianluca Bonitta
Gianluca Lopez
Ilaria Righi
Paolo Mendogni
Margherita Cattaneo
Francesco Damarco
Letizia Morlacchi
Valeria Rossetti
Lorenzo Rosso
Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
Life
lung transplantation
acute cellular rejection
chronic rejection
endoscopic surveillance
transbronchial biopsy
cryobiopsy
title Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
title_full Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
title_fullStr Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
title_full_unstemmed Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
title_short Transition from Transbronchial Forceps to Cryobiopsy After Lung Transplantation: A Single-Centre Experience
title_sort transition from transbronchial forceps to cryobiopsy after lung transplantation a single centre experience
topic lung transplantation
acute cellular rejection
chronic rejection
endoscopic surveillance
transbronchial biopsy
cryobiopsy
url https://www.mdpi.com/2075-1729/14/11/1474
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