Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments
IntroductionThyroid cancer (TC) stands as a prevalent malignancy within the global endocrine system, with its incidence notably exacerbated by the presence of diabetes. However, the specific relationship between TC and diabetes and promising treatment strategies that address both conditions simultan...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2025.1537799/full |
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| author | Siyu Liu Siyu Liu Siyu Liu Yujie Cai Wenjing Chen Xiao Yuan Xiao Yuan Zhipeng He Zhipeng He Zhipeng He Feng Lin Feng Lin |
| author_facet | Siyu Liu Siyu Liu Siyu Liu Yujie Cai Wenjing Chen Xiao Yuan Xiao Yuan Zhipeng He Zhipeng He Zhipeng He Feng Lin Feng Lin |
| author_sort | Siyu Liu |
| collection | DOAJ |
| description | IntroductionThyroid cancer (TC) stands as a prevalent malignancy within the global endocrine system, with its incidence notably exacerbated by the presence of diabetes. However, the specific relationship between TC and diabetes and promising treatment strategies that address both conditions simultaneously are still under exploration. Quercetin, herb medicine from traditional Chinese medicine (TCM), is widely used as an adjunctive therapy with Western medicine in the treatment of many diseases based on a wide range of biological effects. The objective of this study was to explore the efficacy of quercetin in treating TC with diabetes combining bioinformatics and network pharmacology.MethodsAfter multistep Cox proportional hazards regressions, we built a prognostic risk model for TC-diabetes and identified targets for quercetin in treating TC with diabetes. Molecular docking was employed to evaluate the binding affinities of quercetin with core targets, and in vitro experiments verified quercetin’s targets and functions.Results11 prognostic genes were included in the prediction model with a great performance in predicting the prognosis of TC-diabetic patients. 45 genes served as the targets of quercetin in treating TC with diabetes, among which, 5 core genes were screened as the most contributors. Through molecular docking, matrix metalloproteinase-3 (MMP3) was identified as the potential therapeutic target of quercetin. In vitro experiments have found that quercetin can inhibit the proliferation of thyroid cancer cells and the expression of MMP3 under high glucose conditions.DiscussionIn summary, quercetin may suppress the progression of TC-diabetes by inhibiting the proliferation of thyroid cancer cells and the expression of MMP3. |
| format | Article |
| id | doaj-art-d8aaec97a9f044f8b93766be61c0c791 |
| institution | OA Journals |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj-art-d8aaec97a9f044f8b93766be61c0c7912025-08-20T02:33:11ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-06-011610.3389/fendo.2025.15377991537799Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experimentsSiyu Liu0Siyu Liu1Siyu Liu2Yujie Cai3Wenjing Chen4Xiao Yuan5Xiao Yuan6Zhipeng He7Zhipeng He8Zhipeng He9Feng Lin10Feng Lin11Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaAnhui Public Health Clinical Center, Hefei, ChinaDepartment of Radiology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, ChinaSchool of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, ChinaSchool of Pharmacy, Jiangxi Medical College, Nanchang University, Nanchang, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaAnhui Public Health Clinical Center, Hefei, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaAnhui Public Health Clinical Center, Hefei, ChinaDepartment of General Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaAnhui Public Health Clinical Center, Hefei, ChinaIntroductionThyroid cancer (TC) stands as a prevalent malignancy within the global endocrine system, with its incidence notably exacerbated by the presence of diabetes. However, the specific relationship between TC and diabetes and promising treatment strategies that address both conditions simultaneously are still under exploration. Quercetin, herb medicine from traditional Chinese medicine (TCM), is widely used as an adjunctive therapy with Western medicine in the treatment of many diseases based on a wide range of biological effects. The objective of this study was to explore the efficacy of quercetin in treating TC with diabetes combining bioinformatics and network pharmacology.MethodsAfter multistep Cox proportional hazards regressions, we built a prognostic risk model for TC-diabetes and identified targets for quercetin in treating TC with diabetes. Molecular docking was employed to evaluate the binding affinities of quercetin with core targets, and in vitro experiments verified quercetin’s targets and functions.Results11 prognostic genes were included in the prediction model with a great performance in predicting the prognosis of TC-diabetic patients. 45 genes served as the targets of quercetin in treating TC with diabetes, among which, 5 core genes were screened as the most contributors. Through molecular docking, matrix metalloproteinase-3 (MMP3) was identified as the potential therapeutic target of quercetin. In vitro experiments have found that quercetin can inhibit the proliferation of thyroid cancer cells and the expression of MMP3 under high glucose conditions.DiscussionIn summary, quercetin may suppress the progression of TC-diabetes by inhibiting the proliferation of thyroid cancer cells and the expression of MMP3.https://www.frontiersin.org/articles/10.3389/fendo.2025.1537799/fullquercetinthyroid cancerdiabetesnetwork pharmacologybioinformaticsmolecular docking |
| spellingShingle | Siyu Liu Siyu Liu Siyu Liu Yujie Cai Wenjing Chen Xiao Yuan Xiao Yuan Zhipeng He Zhipeng He Zhipeng He Feng Lin Feng Lin Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments Frontiers in Endocrinology quercetin thyroid cancer diabetes network pharmacology bioinformatics molecular docking |
| title | Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| title_full | Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| title_fullStr | Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| title_full_unstemmed | Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| title_short | Mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| title_sort | mechanistic study of quercetin in the treatment of thyroid cancer with diabetes based on network pharmacology and in vitro experiments |
| topic | quercetin thyroid cancer diabetes network pharmacology bioinformatics molecular docking |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1537799/full |
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