Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response.
Identifying new target molecules through which eosinophils secrete their stored proteins may reveal new therapeutic approaches for the control of eosinophilic disorders such as host immune responses to parasites. We have recently reported the expression of the purinergic P2Y12 receptor (P2Y12R) in h...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0139805&type=printable |
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| author | Valdirene S Muniz Renata Baptista-Dos-Reis Claudia F Benjamim Hilton A Mata-Santos Alexandre S Pyrrho Marcelo A Strauch Paulo A Melo Amanda R R Vicentino Juliana Silva-Paiva Christianne Bandeira-Melo Peter F Weller Rodrigo T Figueiredo Josiane S Neves |
| author_facet | Valdirene S Muniz Renata Baptista-Dos-Reis Claudia F Benjamim Hilton A Mata-Santos Alexandre S Pyrrho Marcelo A Strauch Paulo A Melo Amanda R R Vicentino Juliana Silva-Paiva Christianne Bandeira-Melo Peter F Weller Rodrigo T Figueiredo Josiane S Neves |
| author_sort | Valdirene S Muniz |
| collection | DOAJ |
| description | Identifying new target molecules through which eosinophils secrete their stored proteins may reveal new therapeutic approaches for the control of eosinophilic disorders such as host immune responses to parasites. We have recently reported the expression of the purinergic P2Y12 receptor (P2Y12R) in human eosinophils; however, its functional role in this cell type and its involvement in eosinophilic inflammation remain unknown. Here, we investigated functional roles of P2Y12R in isolated human eosinophils and in a murine model of eosinophilic inflammation induced by Schistosoma mansoni (S. mansoni) infection. We found that adenosine 5'-diphosphate (ADP) induced human eosinophils to secrete eosinophil peroxidase (EPO) in a P2Y12R dependent manner. However, ADP did not interfere with human eosinophil apoptosis or chemotaxis in vitro. In vivo, C57Bl/6 mice were infected with cercariae of the Belo Horizonte strain of S. mansoni. Analyses performed 55 days post infection revealed that P2Y12R blockade reduced the granulomatous hepatic area and the eosinophilic infiltrate, collagen deposition and IL-13/IL-4 production in the liver without affecting the parasite oviposition. As found for humans, murine eosinophils also express the P2Y12R. P2Y12R inhibition increased blood eosinophilia, whereas it decreased the bone marrow eosinophil count. Our results suggest that P2Y12R has an important role in eosinophil EPO secretion and in establishing the inflammatory response in the course of a S. mansoni infection. |
| format | Article |
| id | doaj-art-d8a82e81616a458aa59411fdc4e34a16 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d8a82e81616a458aa59411fdc4e34a162025-08-20T03:10:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e013980510.1371/journal.pone.0139805Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response.Valdirene S MunizRenata Baptista-Dos-ReisClaudia F BenjamimHilton A Mata-SantosAlexandre S PyrrhoMarcelo A StrauchPaulo A MeloAmanda R R VicentinoJuliana Silva-PaivaChristianne Bandeira-MeloPeter F WellerRodrigo T FigueiredoJosiane S NevesIdentifying new target molecules through which eosinophils secrete their stored proteins may reveal new therapeutic approaches for the control of eosinophilic disorders such as host immune responses to parasites. We have recently reported the expression of the purinergic P2Y12 receptor (P2Y12R) in human eosinophils; however, its functional role in this cell type and its involvement in eosinophilic inflammation remain unknown. Here, we investigated functional roles of P2Y12R in isolated human eosinophils and in a murine model of eosinophilic inflammation induced by Schistosoma mansoni (S. mansoni) infection. We found that adenosine 5'-diphosphate (ADP) induced human eosinophils to secrete eosinophil peroxidase (EPO) in a P2Y12R dependent manner. However, ADP did not interfere with human eosinophil apoptosis or chemotaxis in vitro. In vivo, C57Bl/6 mice were infected with cercariae of the Belo Horizonte strain of S. mansoni. Analyses performed 55 days post infection revealed that P2Y12R blockade reduced the granulomatous hepatic area and the eosinophilic infiltrate, collagen deposition and IL-13/IL-4 production in the liver without affecting the parasite oviposition. As found for humans, murine eosinophils also express the P2Y12R. P2Y12R inhibition increased blood eosinophilia, whereas it decreased the bone marrow eosinophil count. Our results suggest that P2Y12R has an important role in eosinophil EPO secretion and in establishing the inflammatory response in the course of a S. mansoni infection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0139805&type=printable |
| spellingShingle | Valdirene S Muniz Renata Baptista-Dos-Reis Claudia F Benjamim Hilton A Mata-Santos Alexandre S Pyrrho Marcelo A Strauch Paulo A Melo Amanda R R Vicentino Juliana Silva-Paiva Christianne Bandeira-Melo Peter F Weller Rodrigo T Figueiredo Josiane S Neves Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. PLoS ONE |
| title | Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. |
| title_full | Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. |
| title_fullStr | Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. |
| title_full_unstemmed | Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. |
| title_short | Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response. |
| title_sort | purinergic p2y12 receptor activation in eosinophils and the schistosomal host response |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0139805&type=printable |
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