Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass
Phytopathogenic fungi are primarily responsible for destructive plant diseases that threaten food security. Biological control agents are generally based on their antibiotic characteristics. Nutrient competition is typical in microbes; however, the use of nutrient-competing antagonists for plant dis...
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| Language: | English |
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Elsevier
2025-12-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125002754 |
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| author | Shengyuan Xiao Si-Heng Zhu Rongqiang Cao |
| author_facet | Shengyuan Xiao Si-Heng Zhu Rongqiang Cao |
| author_sort | Shengyuan Xiao |
| collection | DOAJ |
| description | Phytopathogenic fungi are primarily responsible for destructive plant diseases that threaten food security. Biological control agents are generally based on their antibiotic characteristics. Nutrient competition is typical in microbes; however, the use of nutrient-competing antagonists for plant disease control remains underutilized. We found that ginseng root biomass selectively enriches soil pathogens and that biomass depletion prevents pathogen accumulation. We developed a method to capture specific ginseng root biomass-decomposing fungi from soils. We obtained three fungi via this method: one typical ginseng pathogen, Fusarium oxysporum, and two nonpathogenic fungi. These fungi do not display antibiosis to each other. However, nonpathogenic fungi significantly prevent ginseng root biomass-mediated accumulation of F. oxysporum. In addition, all three of these fungi inhibit the changes in the soil fungal community mediated by ginseng root biomass. To validate pathogen inhibition and community manipulation, we tested the effects of a commercial biomass-decomposing fungus, Aspergillus oryzae, on F. oxysporum accumulation and soil fungal community alteration after the addition of ginseng root mixture. The results support our conclusion that this method is simple and effective. Our results highlight an innovative application of nutrient-competing antagonists for plant disease control and a convenient protocol for screening for nutrient-competing antagonists. |
| format | Article |
| id | doaj-art-d89d21a73ff4471ea4e029f9a29f1722 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-d89d21a73ff4471ea4e029f9a29f17222025-08-20T03:32:19ZengElsevierMethodsX2215-01612025-12-011510342910.1016/j.mex.2025.103429Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomassShengyuan Xiao0Si-Heng Zhu1Rongqiang Cao2Engineering Research Centre of the Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, PR China; Corresponding author.Engineering Research Centre of the Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, PR ChinaDepartment of Agriculture Development, Agriculture Bureau of Baishan, Jilin, 134700, PR ChinaPhytopathogenic fungi are primarily responsible for destructive plant diseases that threaten food security. Biological control agents are generally based on their antibiotic characteristics. Nutrient competition is typical in microbes; however, the use of nutrient-competing antagonists for plant disease control remains underutilized. We found that ginseng root biomass selectively enriches soil pathogens and that biomass depletion prevents pathogen accumulation. We developed a method to capture specific ginseng root biomass-decomposing fungi from soils. We obtained three fungi via this method: one typical ginseng pathogen, Fusarium oxysporum, and two nonpathogenic fungi. These fungi do not display antibiosis to each other. However, nonpathogenic fungi significantly prevent ginseng root biomass-mediated accumulation of F. oxysporum. In addition, all three of these fungi inhibit the changes in the soil fungal community mediated by ginseng root biomass. To validate pathogen inhibition and community manipulation, we tested the effects of a commercial biomass-decomposing fungus, Aspergillus oryzae, on F. oxysporum accumulation and soil fungal community alteration after the addition of ginseng root mixture. The results support our conclusion that this method is simple and effective. Our results highlight an innovative application of nutrient-competing antagonists for plant disease control and a convenient protocol for screening for nutrient-competing antagonists.http://www.sciencedirect.com/science/article/pii/S2215016125002754Biological controlling antagonists fishing |
| spellingShingle | Shengyuan Xiao Si-Heng Zhu Rongqiang Cao Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass MethodsX Biological controlling antagonists fishing |
| title | Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass |
| title_full | Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass |
| title_fullStr | Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass |
| title_full_unstemmed | Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass |
| title_short | Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass |
| title_sort | fishing for nutrient competing antagonists for ginseng pathogen control via root biomass |
| topic | Biological controlling antagonists fishing |
| url | http://www.sciencedirect.com/science/article/pii/S2215016125002754 |
| work_keys_str_mv | AT shengyuanxiao fishingfornutrientcompetingantagonistsforginsengpathogencontrolviarootbiomass AT sihengzhu fishingfornutrientcompetingantagonistsforginsengpathogencontrolviarootbiomass AT rongqiangcao fishingfornutrientcompetingantagonistsforginsengpathogencontrolviarootbiomass |