Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms
A new high performance liquid chromatographic (HPLC) method was developed for the determination of paracetamol, phenylephrine hydrochloride, oxolamine citrate and chlorpheniramine maleate in combined pharmaceutical formulations and dosage forms. The separation was performed on an Agilent Zorbax SB-C...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/454325 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683571548094464 |
|---|---|
| author | Ozan Pirol Murat Sukuroglu Tuncel Ozden |
| author_facet | Ozan Pirol Murat Sukuroglu Tuncel Ozden |
| author_sort | Ozan Pirol |
| collection | DOAJ |
| description | A new high performance liquid chromatographic (HPLC) method was developed for the determination of paracetamol, phenylephrine hydrochloride, oxolamine citrate and chlorpheniramine maleate in combined pharmaceutical formulations and dosage forms. The separation was performed on an Agilent Zorbax SB-CN column with the mobile phase consisting of 0.02 M phosphate buffer (pH:4) and acetonitrile (85:15,v/v) in flow rate 1.5 mL at 22 °C. The overall retention time of the analytes was 3.5 min. The method was validated with respect to linearity, precision, accuracy and recovery. The relative standard deviation for 10 replicate measurements of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were 0.12, 0.36 0.18 and 0.59%, respectively. Total recoveries of analytes were 99.99, 100.56, 100.20 and 99.60%, respectively. No chromatographic interference from the tablet excipients was found. The linearity of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were in the range of 20-120 μg/mL, 0.4-2.4 μg/mL, 8-48 μg/mL and 0.16-0.96 μg/mL, respectively. This simple, fast, economical and precise high performance liquid chromatographic method can be adopted for routine quality control analysis. |
| format | Article |
| id | doaj-art-d88ebd9f363e4dbb874ccfb4da423fe2 |
| institution | DOAJ |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-d88ebd9f363e4dbb874ccfb4da423fe22025-08-20T03:23:50ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01831275127910.1155/2011/454325Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage FormsOzan Pirol0Murat Sukuroglu1Tuncel Ozden2Gazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330, TurkeyGazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330, TurkeyGazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330, TurkeyA new high performance liquid chromatographic (HPLC) method was developed for the determination of paracetamol, phenylephrine hydrochloride, oxolamine citrate and chlorpheniramine maleate in combined pharmaceutical formulations and dosage forms. The separation was performed on an Agilent Zorbax SB-CN column with the mobile phase consisting of 0.02 M phosphate buffer (pH:4) and acetonitrile (85:15,v/v) in flow rate 1.5 mL at 22 °C. The overall retention time of the analytes was 3.5 min. The method was validated with respect to linearity, precision, accuracy and recovery. The relative standard deviation for 10 replicate measurements of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were 0.12, 0.36 0.18 and 0.59%, respectively. Total recoveries of analytes were 99.99, 100.56, 100.20 and 99.60%, respectively. No chromatographic interference from the tablet excipients was found. The linearity of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were in the range of 20-120 μg/mL, 0.4-2.4 μg/mL, 8-48 μg/mL and 0.16-0.96 μg/mL, respectively. This simple, fast, economical and precise high performance liquid chromatographic method can be adopted for routine quality control analysis.http://dx.doi.org/10.1155/2011/454325 |
| spellingShingle | Ozan Pirol Murat Sukuroglu Tuncel Ozden Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms E-Journal of Chemistry |
| title | Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms |
| title_full | Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms |
| title_fullStr | Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms |
| title_full_unstemmed | Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms |
| title_short | Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms |
| title_sort | simultaneous determination of paracetamol phenylephrine hydrochloride oxolamine citrate and chlorpheniramine maleate by hplc in pharmaceutical dosage forms |
| url | http://dx.doi.org/10.1155/2011/454325 |
| work_keys_str_mv | AT ozanpirol simultaneousdeterminationofparacetamolphenylephrinehydrochlorideoxolaminecitrateandchlorpheniraminemaleatebyhplcinpharmaceuticaldosageforms AT muratsukuroglu simultaneousdeterminationofparacetamolphenylephrinehydrochlorideoxolaminecitrateandchlorpheniraminemaleatebyhplcinpharmaceuticaldosageforms AT tuncelozden simultaneousdeterminationofparacetamolphenylephrinehydrochlorideoxolaminecitrateandchlorpheniraminemaleatebyhplcinpharmaceuticaldosageforms |