Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns
Abstract Corneal alkali burns can cause persistent inflammation and corneal neovascularization. In this study, we divided corneal alkali burned rabbits into the untreated group, the blank lens group, the radiation-treated umbilical cord mesenchymal stem cells (UCMSC) lens group, and the UCMSC I.V. g...
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Nature Portfolio
2025-03-01
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| author | Siqi Song Yaqi Cheng Weihua Li Huan Yu Zhiquan Li Jianbing Li Meng Li Qunai Huang Yingjie Liu Shiqi Ling |
| author_facet | Siqi Song Yaqi Cheng Weihua Li Huan Yu Zhiquan Li Jianbing Li Meng Li Qunai Huang Yingjie Liu Shiqi Ling |
| author_sort | Siqi Song |
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| description | Abstract Corneal alkali burns can cause persistent inflammation and corneal neovascularization. In this study, we divided corneal alkali burned rabbits into the untreated group, the blank lens group, the radiation-treated umbilical cord mesenchymal stem cells (UCMSC) lens group, and the UCMSC I.V. group, and then measured corneal inflammation, neovascularization and corneal injury repair via slit lamp microscopy, captured anterior segment optical coherence tomography (AS-OCT), and performed hematoxylin-eosin staining. Compared with those in the other experimental groups, radiation-treated UCMSC lenses significantly decreased inflammatory index (IF) scores, areas of corneal blood vessels and corneal epithelial injury. The expression of interleukin (IL)-17 in corneas treated with radiation-treated UCMSC lenses was lower than that in corneas treated with blank lenses, and radiation-treated UCMSC lenses exhibited greater expression of IL-4 and signal transducer and activator of transcription 1 (STAT1), while the expression of cluster of differentiation-3G (CD3G), a linker for the activation of T cells (LAT), IL-6, IL-1B, CC chemokine receptor 6 (CCR6) and IL-23 exhibited the opposite effects (all P < 0.05). Our findings demonstrated that irradiated UCMSC-coated high oxygen-permeable hydrogel lenses on the ocular surface inhibited corneal angiogenesis and inflammation after corneal alkaline burns. The downregulation of Th17 cell differentiation might be responsible for these effects. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-d88e99b1effb49d8bb072f4c1a1ca4012025-08-20T03:40:49ZengNature PortfolioScientific Reports2045-23222025-03-0115111710.1038/s41598-025-95007-9Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burnsSiqi Song0Yaqi Cheng1Weihua Li2Huan Yu3Zhiquan Li4Jianbing Li5Meng Li6Qunai Huang7Yingjie Liu8Shiqi Ling9Department of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen UniversityAbstract Corneal alkali burns can cause persistent inflammation and corneal neovascularization. In this study, we divided corneal alkali burned rabbits into the untreated group, the blank lens group, the radiation-treated umbilical cord mesenchymal stem cells (UCMSC) lens group, and the UCMSC I.V. group, and then measured corneal inflammation, neovascularization and corneal injury repair via slit lamp microscopy, captured anterior segment optical coherence tomography (AS-OCT), and performed hematoxylin-eosin staining. Compared with those in the other experimental groups, radiation-treated UCMSC lenses significantly decreased inflammatory index (IF) scores, areas of corneal blood vessels and corneal epithelial injury. The expression of interleukin (IL)-17 in corneas treated with radiation-treated UCMSC lenses was lower than that in corneas treated with blank lenses, and radiation-treated UCMSC lenses exhibited greater expression of IL-4 and signal transducer and activator of transcription 1 (STAT1), while the expression of cluster of differentiation-3G (CD3G), a linker for the activation of T cells (LAT), IL-6, IL-1B, CC chemokine receptor 6 (CCR6) and IL-23 exhibited the opposite effects (all P < 0.05). Our findings demonstrated that irradiated UCMSC-coated high oxygen-permeable hydrogel lenses on the ocular surface inhibited corneal angiogenesis and inflammation after corneal alkaline burns. The downregulation of Th17 cell differentiation might be responsible for these effects.https://doi.org/10.1038/s41598-025-95007-9Radiation-treated umbilical cord stem cellsAlkali corneal burnsInflammationAngiogenesisTh17 cell differentiation |
| spellingShingle | Siqi Song Yaqi Cheng Weihua Li Huan Yu Zhiquan Li Jianbing Li Meng Li Qunai Huang Yingjie Liu Shiqi Ling Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns Scientific Reports Radiation-treated umbilical cord stem cells Alkali corneal burns Inflammation Angiogenesis Th17 cell differentiation |
| title | Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| title_full | Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| title_fullStr | Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| title_full_unstemmed | Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| title_short | Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| title_sort | irradiated umbilical cord mesenchymal stem cell coated high oxygen permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns |
| topic | Radiation-treated umbilical cord stem cells Alkali corneal burns Inflammation Angiogenesis Th17 cell differentiation |
| url | https://doi.org/10.1038/s41598-025-95007-9 |
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