Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests
The objective of this study was to assess the genetic variability in two natural populations of Calophyllum brasiliense located along two different rivers in the state of Minas Gerais, Brazil, using RAPD molecular markers. Eighty-two polymorphic fragments were amplified using 27 primers. The values...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Forestry Research |
| Online Access: | http://dx.doi.org/10.1155/2014/305286 |
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| author | Evânia Galvão Mendonça Anderson Marcos de Souza Fábio de Almeida Vieira Regiane Abjaud Estopa Cristiane Aparecida Fioravante Reis Dulcinéia de Carvalho |
| author_facet | Evânia Galvão Mendonça Anderson Marcos de Souza Fábio de Almeida Vieira Regiane Abjaud Estopa Cristiane Aparecida Fioravante Reis Dulcinéia de Carvalho |
| author_sort | Evânia Galvão Mendonça |
| collection | DOAJ |
| description | The objective of this study was to assess the genetic variability in two natural populations of Calophyllum brasiliense located along two different rivers in the state of Minas Gerais, Brazil, using RAPD molecular markers. Eighty-two polymorphic fragments were amplified using 27 primers. The values obtained for Shannon index (I) were 0.513 and 0.530 for the populations located on the margins of the Rio Grande and Rio das Mortes, respectively, demonstrating the high genetic diversity in the studied populations. Nei’s genetic diversity (He) was 0.341 for the Rio Grande population and 0.357 for the Rio das Mortes population. These results were not significantly different between populations and suggest a large proportion of heterozygote individuals within both populations. AMOVA showed that 70.42% of the genetic variability is found within populations and 29.58% is found among populations (ФST=0.2958). The analysis of kinship coefficients detected the existence of family structures in both populations. Average kinship coefficients between neighboring individuals were 0.053 (P<0.001) in Rio das Mortes and 0.040 (P<0.001) in Rio Grande. This could be due to restricted pollen and seed dispersal and the history of anthropogenic disturbance in the area. These factors are likely to contribute to the relatedness observed among these genotypes. |
| format | Article |
| id | doaj-art-d87b8eb50d8542fea54920f9be160aba |
| institution | OA Journals |
| issn | 1687-9368 1687-9376 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | International Journal of Forestry Research |
| spelling | doaj-art-d87b8eb50d8542fea54920f9be160aba2025-08-20T02:20:57ZengWileyInternational Journal of Forestry Research1687-93681687-93762014-01-01201410.1155/2014/305286305286Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian ForestsEvânia Galvão Mendonça0Anderson Marcos de Souza1Fábio de Almeida Vieira2Regiane Abjaud Estopa3Cristiane Aparecida Fioravante Reis4Dulcinéia de Carvalho5Departamento de Engenharia Florestal, Universidade Federal Rural do Rio de Janeiro, 23890-000 Rio de Janeiro, RN, BrazilDepartamento de Engenharia Florestal, Universidade de Brasília, 70919-900 Brasília, DF, BrazilUnidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do Norte, 59280-000 Macaíba, RN, BrazilKlabin, Avenida Brasil 26, Bairro Harmonia, 84275-000 Telêmaco Borba, PR, BrazilEmbrapa Florestas, 74001-970 Goiânia, GO, BrazilDepartamento de Ciências Florestais, Universidade Federal de Lavras, 37200-000 Lavras, MG, BrazilThe objective of this study was to assess the genetic variability in two natural populations of Calophyllum brasiliense located along two different rivers in the state of Minas Gerais, Brazil, using RAPD molecular markers. Eighty-two polymorphic fragments were amplified using 27 primers. The values obtained for Shannon index (I) were 0.513 and 0.530 for the populations located on the margins of the Rio Grande and Rio das Mortes, respectively, demonstrating the high genetic diversity in the studied populations. Nei’s genetic diversity (He) was 0.341 for the Rio Grande population and 0.357 for the Rio das Mortes population. These results were not significantly different between populations and suggest a large proportion of heterozygote individuals within both populations. AMOVA showed that 70.42% of the genetic variability is found within populations and 29.58% is found among populations (ФST=0.2958). The analysis of kinship coefficients detected the existence of family structures in both populations. Average kinship coefficients between neighboring individuals were 0.053 (P<0.001) in Rio das Mortes and 0.040 (P<0.001) in Rio Grande. This could be due to restricted pollen and seed dispersal and the history of anthropogenic disturbance in the area. These factors are likely to contribute to the relatedness observed among these genotypes.http://dx.doi.org/10.1155/2014/305286 |
| spellingShingle | Evânia Galvão Mendonça Anderson Marcos de Souza Fábio de Almeida Vieira Regiane Abjaud Estopa Cristiane Aparecida Fioravante Reis Dulcinéia de Carvalho Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests International Journal of Forestry Research |
| title | Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests |
| title_full | Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests |
| title_fullStr | Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests |
| title_full_unstemmed | Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests |
| title_short | Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of Natural Calophyllum brasiliense (Clusiaceae) Populations in Riparian Forests |
| title_sort | using random amplified polymorphic dna to assess genetic diversity and structure of natural calophyllum brasiliense clusiaceae populations in riparian forests |
| url | http://dx.doi.org/10.1155/2014/305286 |
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