MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair
ObjectiveThis study aimed to evaluate the effectiveness of bone marrow mesenchymal stem cells (BMSCs) combined with sodium alginate scaffolds in repairing knee cartilage defects in New Zealand rabbits. Additionally, it assessed the potential of functional magnetic resonance imaging (fMRI) for non-in...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-03-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554292/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849774221788446720 |
|---|---|
| author | Shanyu Lu Shanyu Lu Zhenyu Liu Zhenyu Liu Meiling Qi Meiling Qi Haocheng Zhen Jing Luo Yingchao Wang Yingchao Wang Le Chang Le Chang Xiaolong Bai Xiaolong Bai Yingguang Jiao Yingguang Jiao Xinyao Chen Xinyao Chen Junping Zhen Junping Zhen |
| author_facet | Shanyu Lu Shanyu Lu Zhenyu Liu Zhenyu Liu Meiling Qi Meiling Qi Haocheng Zhen Jing Luo Yingchao Wang Yingchao Wang Le Chang Le Chang Xiaolong Bai Xiaolong Bai Yingguang Jiao Yingguang Jiao Xinyao Chen Xinyao Chen Junping Zhen Junping Zhen |
| author_sort | Shanyu Lu |
| collection | DOAJ |
| description | ObjectiveThis study aimed to evaluate the effectiveness of bone marrow mesenchymal stem cells (BMSCs) combined with sodium alginate scaffolds in repairing knee cartilage defects in New Zealand rabbits. Additionally, it assessed the potential of functional magnetic resonance imaging (fMRI) for non-invasive monitoring of the dynamic repair process.MethodsRabbits were randomly divided into four groups: Group A (control), Group B (sodium alginate scaffold), Group C (BMSCs-sodium alginate scaffold), and Group D (USPIO-labeled BMSCs-sodium alginate scaffold). A cartilage defect model was created, and the respective materials were implanted into the defect regions. T2 mapping MRI was performed at weeks 1, 2, and 4 post-surgery to evaluate the repair process, followed by histological analysis to confirm the outcomes.ResultsBMSCs significantly promoted cartilage defect repair and accelerated the degradation of sodium alginate scaffolds. Macroscopic and histological evaluations revealed repair tissue formation in Groups C and D by week 1, with most defect regions filled with new cartilage by week 4. T2 mapping analysis showed a gradual decline in T2 values in Group B, a more pronounced decrease in Group C, and consistently lower T2 values in Group D compared to Group C, with a slow upward trend over time.ConclusionThis study demonstrated that BMSCs exhibit significant regenerative potential for cartilage defect repair. USPIO labeling enables non-invasive, dynamic monitoring of the repair process without adverse effects on cell viability or differentiation. These findings provide experimental evidence supporting the application of BMSCs combined with magnetic labeling technology in cartilage regeneration. |
| format | Article |
| id | doaj-art-d875fb02c75a441588366ec727f72303 |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-d875fb02c75a441588366ec727f723032025-08-20T03:01:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-03-011310.3389/fbioe.2025.15542921554292MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repairShanyu Lu0Shanyu Lu1Zhenyu Liu2Zhenyu Liu3Meiling Qi4Meiling Qi5Haocheng Zhen6Jing Luo7Yingchao Wang8Yingchao Wang9Le Chang10Le Chang11Xiaolong Bai12Xiaolong Bai13Yingguang Jiao14Yingguang Jiao15Xinyao Chen16Xinyao Chen17Junping Zhen18Junping Zhen19College of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaClinical and Basic Medical College, Shandong First Medical University, Jinan, Shandong, ChinaShanxi Key Laboratory for Immunomicroecology, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaCollege of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Imaging, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaMolecular Imaging Laboratory, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaObjectiveThis study aimed to evaluate the effectiveness of bone marrow mesenchymal stem cells (BMSCs) combined with sodium alginate scaffolds in repairing knee cartilage defects in New Zealand rabbits. Additionally, it assessed the potential of functional magnetic resonance imaging (fMRI) for non-invasive monitoring of the dynamic repair process.MethodsRabbits were randomly divided into four groups: Group A (control), Group B (sodium alginate scaffold), Group C (BMSCs-sodium alginate scaffold), and Group D (USPIO-labeled BMSCs-sodium alginate scaffold). A cartilage defect model was created, and the respective materials were implanted into the defect regions. T2 mapping MRI was performed at weeks 1, 2, and 4 post-surgery to evaluate the repair process, followed by histological analysis to confirm the outcomes.ResultsBMSCs significantly promoted cartilage defect repair and accelerated the degradation of sodium alginate scaffolds. Macroscopic and histological evaluations revealed repair tissue formation in Groups C and D by week 1, with most defect regions filled with new cartilage by week 4. T2 mapping analysis showed a gradual decline in T2 values in Group B, a more pronounced decrease in Group C, and consistently lower T2 values in Group D compared to Group C, with a slow upward trend over time.ConclusionThis study demonstrated that BMSCs exhibit significant regenerative potential for cartilage defect repair. USPIO labeling enables non-invasive, dynamic monitoring of the repair process without adverse effects on cell viability or differentiation. These findings provide experimental evidence supporting the application of BMSCs combined with magnetic labeling technology in cartilage regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554292/fullcartilage repairT2 mapping MRIUSPIObone marrow mesenchymal stem cellsnon-invasive monitoring |
| spellingShingle | Shanyu Lu Shanyu Lu Zhenyu Liu Zhenyu Liu Meiling Qi Meiling Qi Haocheng Zhen Jing Luo Yingchao Wang Yingchao Wang Le Chang Le Chang Xiaolong Bai Xiaolong Bai Yingguang Jiao Yingguang Jiao Xinyao Chen Xinyao Chen Junping Zhen Junping Zhen MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair Frontiers in Bioengineering and Biotechnology cartilage repair T2 mapping MRI USPIO bone marrow mesenchymal stem cells non-invasive monitoring |
| title | MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair |
| title_full | MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair |
| title_fullStr | MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair |
| title_full_unstemmed | MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair |
| title_short | MRI monitoring of USPIO-labeled BMSCs combined with alginate scaffold for cartilage defect repair |
| title_sort | mri monitoring of uspio labeled bmscs combined with alginate scaffold for cartilage defect repair |
| topic | cartilage repair T2 mapping MRI USPIO bone marrow mesenchymal stem cells non-invasive monitoring |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554292/full |
| work_keys_str_mv | AT shanyulu mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT shanyulu mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT zhenyuliu mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT zhenyuliu mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT meilingqi mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT meilingqi mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT haochengzhen mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT jingluo mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT yingchaowang mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT yingchaowang mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT lechang mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT lechang mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT xiaolongbai mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT xiaolongbai mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT yingguangjiao mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT yingguangjiao mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT xinyaochen mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT xinyaochen mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT junpingzhen mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair AT junpingzhen mrimonitoringofuspiolabeledbmscscombinedwithalginatescaffoldforcartilagedefectrepair |