Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions
Plasma amino acid (PAA) concentration in horses vary according to the exercise type. This study evaluated the changes in PAA levels and the associated metabolites, urea and ammonia, following short-duration, high-intensity cross-country exercise in eventing horses. Twenty eventing horses participate...
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MDPI AG
2025-06-01
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| author | Flora Philine Reemtsma Johanna Giers Stephanie Horstmann Sabita Diana Stoeckle Heidrun Gehlen |
| author_facet | Flora Philine Reemtsma Johanna Giers Stephanie Horstmann Sabita Diana Stoeckle Heidrun Gehlen |
| author_sort | Flora Philine Reemtsma |
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| description | Plasma amino acid (PAA) concentration in horses vary according to the exercise type. This study evaluated the changes in PAA levels and the associated metabolites, urea and ammonia, following short-duration, high-intensity cross-country exercise in eventing horses. Twenty eventing horses participated in 55 rides at 14 international competitions (2* to 4* levels) across five venues in Germany and Poland. Blood samples were collected at four timepoints: before exercise (TP0), at 10 min (TP1), and at 30 min (TP2) post-exercise, as well as in the morning on the day after the competition (TP3). A total of 23 different PAAs and two metabolites (ammonia and urea) were analyzed. PAA concentration difference over time was assessed by a mixed ANOVA. Significant fluctuations were observed in 18/25 parameters. For 21/23 PAAs, levels increased at TP1 and/or TP2, while cysteine concentrations decreased. Concentrations returned to pre-competition levels for 21/23 PAAs by TP3. Proline levels remained elevated (<i>p</i> = 0.002), while those of glycine significantly decreased (<i>p</i> = 0.027) at TP3. Plasma ammonia and urea levels increased at TP1, TP2 and TP3. This study provides foundations for supplementation strategies and can inform future works exploring PAAs’ role in performance and training adaptation in eventing horses and their potential as performance-related biomarkers. |
| format | Article |
| id | doaj-art-d87148f611db49b8b464161c7d3106a2 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-d87148f611db49b8b464161c7d3106a22025-08-20T03:50:21ZengMDPI AGAnimals2076-26152025-06-011513184010.3390/ani15131840Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country CompetitionsFlora Philine Reemtsma0Johanna Giers1Stephanie Horstmann2Sabita Diana Stoeckle3Heidrun Gehlen4Equine Clinic, Internal Medicine, Freie Universität Berlin, Oertzenweg 19b, 14193 Berlin, GermanyTierklinik Großmoor, Holzweg 13, 29352 Adelheidsdorf, GermanyGerman Olympic Committee for Equestrian Sports (DOKR), Freiherr-von-Langen-Straße 15, 48231 Warendorf, GermanyEquine Clinic, Internal Medicine, Freie Universität Berlin, Oertzenweg 19b, 14193 Berlin, GermanyEquine Clinic, Internal Medicine, Freie Universität Berlin, Oertzenweg 19b, 14193 Berlin, GermanyPlasma amino acid (PAA) concentration in horses vary according to the exercise type. This study evaluated the changes in PAA levels and the associated metabolites, urea and ammonia, following short-duration, high-intensity cross-country exercise in eventing horses. Twenty eventing horses participated in 55 rides at 14 international competitions (2* to 4* levels) across five venues in Germany and Poland. Blood samples were collected at four timepoints: before exercise (TP0), at 10 min (TP1), and at 30 min (TP2) post-exercise, as well as in the morning on the day after the competition (TP3). A total of 23 different PAAs and two metabolites (ammonia and urea) were analyzed. PAA concentration difference over time was assessed by a mixed ANOVA. Significant fluctuations were observed in 18/25 parameters. For 21/23 PAAs, levels increased at TP1 and/or TP2, while cysteine concentrations decreased. Concentrations returned to pre-competition levels for 21/23 PAAs by TP3. Proline levels remained elevated (<i>p</i> = 0.002), while those of glycine significantly decreased (<i>p</i> = 0.027) at TP3. Plasma ammonia and urea levels increased at TP1, TP2 and TP3. This study provides foundations for supplementation strategies and can inform future works exploring PAAs’ role in performance and training adaptation in eventing horses and their potential as performance-related biomarkers.https://www.mdpi.com/2076-2615/15/13/1840equineamino acidsexerciseperformanceathletefitness |
| spellingShingle | Flora Philine Reemtsma Johanna Giers Stephanie Horstmann Sabita Diana Stoeckle Heidrun Gehlen Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions Animals equine amino acids exercise performance athlete fitness |
| title | Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions |
| title_full | Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions |
| title_fullStr | Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions |
| title_full_unstemmed | Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions |
| title_short | Concentration Changes in Plasma Amino Acids and Their Metabolites in Eventing Horses During Cross-Country Competitions |
| title_sort | concentration changes in plasma amino acids and their metabolites in eventing horses during cross country competitions |
| topic | equine amino acids exercise performance athlete fitness |
| url | https://www.mdpi.com/2076-2615/15/13/1840 |
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