Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats

Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this st...

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Main Authors: Lidan Hu, Lili Yu, Zhongkai Cao, Yue Wang, Caifeng Zhu, Yayu Li, Jiazhen Yin, Zhichao Ma, Xuelin He, Ying Zhang, Wunan Huang, Yuelin Guan, Yue Chen, Xue Li, Xiangjun Chen
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Language:English
Published: Elsevier 2024-12-01
Series:Journal of Pharmaceutical Analysis
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095177924002028
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author Lidan Hu
Lili Yu
Zhongkai Cao
Yue Wang
Caifeng Zhu
Yayu Li
Jiazhen Yin
Zhichao Ma
Xuelin He
Ying Zhang
Wunan Huang
Yuelin Guan
Yue Chen
Xue Li
Xiangjun Chen
author_facet Lidan Hu
Lili Yu
Zhongkai Cao
Yue Wang
Caifeng Zhu
Yayu Li
Jiazhen Yin
Zhichao Ma
Xuelin He
Ying Zhang
Wunan Huang
Yuelin Guan
Yue Chen
Xue Li
Xiangjun Chen
author_sort Lidan Hu
collection DOAJ
description Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this study, DN was induced in Sprague-Dawley rats using streptozotocin (STZ) and a high-fat diet (HFD), and the protective mechanisms of GLP were investigated through transcriptomic, metabolomic, and network pharmacology (NP) analyses. Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats. Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes. GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression (Igfbp1 and Angptl4) and enhanced metabolic biomarkers of 4-Aminocatechol. In addition, NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways. In conclusion, this study provides supportive evidence of the anti-inflammatory effects of GLP supplements, highlighting their potential for promising clinical applications in treating DN.
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spelling doaj-art-d869a9bfa7654de2b01d86d509526bcd2025-01-30T05:13:59ZengElsevierJournal of Pharmaceutical Analysis2095-17792024-12-011412101105Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy ratsLidan Hu0Lili Yu1Zhongkai Cao2Yue Wang3Caifeng Zhu4Yayu Li5Jiazhen Yin6Zhichao Ma7Xuelin He8Ying Zhang9Wunan Huang10Yuelin Guan11Yue Chen12Xue Li13Xiangjun Chen14Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaCentre for Global Health, Usher Institute, The University of Edinburgh, Edinburgh, EH16 4UX, UK; Department of Big Data in Health Science School of Public Health, Zhejiang University School of Medicine, Hangzhou, 310058, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaHubei Normal University, Huangshi, Hubei, 435002, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaKidney Disease Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, ChinaInstitute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, 310020, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaDepartment of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, 300211, China; Corresponding author.Department of Big Data in Health Science School of Public Health, Zhejiang University School of Medicine, Hangzhou, 310058, China; Corresponding author.Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, 310020, China; Corresponding author.Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this study, DN was induced in Sprague-Dawley rats using streptozotocin (STZ) and a high-fat diet (HFD), and the protective mechanisms of GLP were investigated through transcriptomic, metabolomic, and network pharmacology (NP) analyses. Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats. Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes. GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression (Igfbp1 and Angptl4) and enhanced metabolic biomarkers of 4-Aminocatechol. In addition, NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways. In conclusion, this study provides supportive evidence of the anti-inflammatory effects of GLP supplements, highlighting their potential for promising clinical applications in treating DN.http://www.sciencedirect.com/science/article/pii/S2095177924002028Diabetic nephropathyMetabolomeNetwork pharmacologySporoderm-broken spores of Ganoderma lucidumTranscriptome
spellingShingle Lidan Hu
Lili Yu
Zhongkai Cao
Yue Wang
Caifeng Zhu
Yayu Li
Jiazhen Yin
Zhichao Ma
Xuelin He
Ying Zhang
Wunan Huang
Yuelin Guan
Yue Chen
Xue Li
Xiangjun Chen
Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
Journal of Pharmaceutical Analysis
Diabetic nephropathy
Metabolome
Network pharmacology
Sporoderm-broken spores of Ganoderma lucidum
Transcriptome
title Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
title_full Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
title_fullStr Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
title_full_unstemmed Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
title_short Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
title_sort integrating transcriptomics metabolomics and network pharmacology to investigate multi target effects of sporoderm broken spores of ganoderma lucidum on improving hfd induced diabetic nephropathy rats
topic Diabetic nephropathy
Metabolome
Network pharmacology
Sporoderm-broken spores of Ganoderma lucidum
Transcriptome
url http://www.sciencedirect.com/science/article/pii/S2095177924002028
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