Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption
Magnetic nanoparticles (MNPs) are extensively utilized in biomedicine as part of controlled drug release systems, hyperthermia, and magnetic resonance imaging. Surface modification of MNPs not only enhances their stability and biocompatibility but also increases affinity with certain molecules, allo...
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AIMS Press
2024-10-01
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| Series: | AIMS Materials Science |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/matersci.2024047 |
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| author | Denise Arrozarena Portilla Arturo A. Velázquez López Rosalva Mora Escobedo Hernani Yee Madeira |
| author_facet | Denise Arrozarena Portilla Arturo A. Velázquez López Rosalva Mora Escobedo Hernani Yee Madeira |
| author_sort | Denise Arrozarena Portilla |
| collection | DOAJ |
| description | Magnetic nanoparticles (MNPs) are extensively utilized in biomedicine as part of controlled drug release systems, hyperthermia, and magnetic resonance imaging. Surface modification of MNPs not only enhances their stability and biocompatibility but also increases affinity with certain molecules, allowing them to be used in protein separation and adsorption processes. This article reports the synthesis and characterization of iron oxide MNPs functionalized with citric acid (IONPs@CA) to evaluate their performance in protein adsorption. The nanoparticles were characterized using various techniques such as transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), thermogravimetric analysis (TGA), and Fourier-transform infrared spectroscopy (FT-IR). The percentage of lysozyme (Lyz) adsorbed by IONPs@CA was 84.9%, while the IONPs sample only adsorbed 5.9%. In silico evaluation results showed some repulsion bonds obtained in Lyz-IONPs and hydrogen bonds, carbon-hydrogen bonds, and van der Waals interactions in Lyz-IONPs@CA. These results may be novel since no previous research was found specifying this type of interaction between lysozyme and IONPs and/or IONPs@CA. The maximum adsorption efficiency obtained for the coated nanoparticles was 88.3%. |
| format | Article |
| id | doaj-art-d866b59540f34bc5b411400d327b8c36 |
| institution | Kabale University |
| issn | 2372-0484 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Materials Science |
| spelling | doaj-art-d866b59540f34bc5b411400d327b8c362025-01-24T01:30:26ZengAIMS PressAIMS Materials Science2372-04842024-10-01115991101210.3934/matersci.2024047Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorptionDenise Arrozarena Portilla0Arturo A. Velázquez López1Rosalva Mora Escobedo2Hernani Yee Madeira3Laboratorio de Espectroscopía Mossbauer y Técnicas Complementarias, Departamento de Física, Escuela Superior de Físico Matemáticas, Instituto Politécnico Nacional, Ciudad de México 07738, MéxicoLaboratorio de Bioquímica de la Nutrición, Departamento de Ingeniería Bioquímica, Escuela nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 07738, MéxicoLaboratorio de Bioquímica de la Nutrición, Departamento de Ingeniería Bioquímica, Escuela nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 07738, MéxicoLaboratorio de Espectroscopía Mossbauer y Técnicas Complementarias, Departamento de Física, Escuela Superior de Físico Matemáticas, Instituto Politécnico Nacional, Ciudad de México 07738, MéxicoMagnetic nanoparticles (MNPs) are extensively utilized in biomedicine as part of controlled drug release systems, hyperthermia, and magnetic resonance imaging. Surface modification of MNPs not only enhances their stability and biocompatibility but also increases affinity with certain molecules, allowing them to be used in protein separation and adsorption processes. This article reports the synthesis and characterization of iron oxide MNPs functionalized with citric acid (IONPs@CA) to evaluate their performance in protein adsorption. The nanoparticles were characterized using various techniques such as transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), thermogravimetric analysis (TGA), and Fourier-transform infrared spectroscopy (FT-IR). The percentage of lysozyme (Lyz) adsorbed by IONPs@CA was 84.9%, while the IONPs sample only adsorbed 5.9%. In silico evaluation results showed some repulsion bonds obtained in Lyz-IONPs and hydrogen bonds, carbon-hydrogen bonds, and van der Waals interactions in Lyz-IONPs@CA. These results may be novel since no previous research was found specifying this type of interaction between lysozyme and IONPs and/or IONPs@CA. The maximum adsorption efficiency obtained for the coated nanoparticles was 88.3%.https://www.aimspress.com/article/doi/10.3934/matersci.2024047iron oxide nanoparticlescitric acid functionalizationprotein purificationin silico study |
| spellingShingle | Denise Arrozarena Portilla Arturo A. Velázquez López Rosalva Mora Escobedo Hernani Yee Madeira Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption AIMS Materials Science iron oxide nanoparticles citric acid functionalization protein purification in silico study |
| title | Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption |
| title_full | Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption |
| title_fullStr | Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption |
| title_full_unstemmed | Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption |
| title_short | Citrate coated iron oxide nanoparticles: Synthesis, characterization, and performance in protein adsorption |
| title_sort | citrate coated iron oxide nanoparticles synthesis characterization and performance in protein adsorption |
| topic | iron oxide nanoparticles citric acid functionalization protein purification in silico study |
| url | https://www.aimspress.com/article/doi/10.3934/matersci.2024047 |
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