Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells.
Activity of separase, a cysteine protease that cleaves sister chromatid cohesin at the onset of anaphase, is tightly regulated to ensure faithful chromosome segregation and genome stability. Two mechanisms negatively regulate separase: inhibition by securin and phosphorylation on serine 1121. To gau...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060015&type=printable |
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| _version_ | 1849686706306940928 |
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| author | Xingxu Huang Claudia V Andreu-Vieyra J Philippe York Rashieda Hatcher Tao Lu Martin M Matzuk Pumin Zhang |
| author_facet | Xingxu Huang Claudia V Andreu-Vieyra J Philippe York Rashieda Hatcher Tao Lu Martin M Matzuk Pumin Zhang |
| author_sort | Xingxu Huang |
| collection | DOAJ |
| description | Activity of separase, a cysteine protease that cleaves sister chromatid cohesin at the onset of anaphase, is tightly regulated to ensure faithful chromosome segregation and genome stability. Two mechanisms negatively regulate separase: inhibition by securin and phosphorylation on serine 1121. To gauge the physiological significance of the inhibitory phosphorylation, we created a mouse strain in which Ser1121 was mutated to Ala (S1121A). Here we report that this S1121A point mutation causes infertility in mice. We show that germ cells in the mutants are depleted during development. We further demonstrate that S1121A causes chromosome misalignment during proliferation of the postmigratory primordial germ cells, resulting in mitotic arrest, aneuploidy, and eventual cell death. Our results indicate that inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells. |
| format | Article |
| id | doaj-art-d85fe5d4661f4440a5cf9952d83a8208 |
| institution | DOAJ |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-d85fe5d4661f4440a5cf9952d83a82082025-08-20T03:22:35ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852008-01-0161e1510.1371/journal.pbio.0060015Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells.Xingxu HuangClaudia V Andreu-VieyraJ Philippe YorkRashieda HatcherTao LuMartin M MatzukPumin ZhangActivity of separase, a cysteine protease that cleaves sister chromatid cohesin at the onset of anaphase, is tightly regulated to ensure faithful chromosome segregation and genome stability. Two mechanisms negatively regulate separase: inhibition by securin and phosphorylation on serine 1121. To gauge the physiological significance of the inhibitory phosphorylation, we created a mouse strain in which Ser1121 was mutated to Ala (S1121A). Here we report that this S1121A point mutation causes infertility in mice. We show that germ cells in the mutants are depleted during development. We further demonstrate that S1121A causes chromosome misalignment during proliferation of the postmigratory primordial germ cells, resulting in mitotic arrest, aneuploidy, and eventual cell death. Our results indicate that inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060015&type=printable |
| spellingShingle | Xingxu Huang Claudia V Andreu-Vieyra J Philippe York Rashieda Hatcher Tao Lu Martin M Matzuk Pumin Zhang Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. PLoS Biology |
| title | Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. |
| title_full | Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. |
| title_fullStr | Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. |
| title_full_unstemmed | Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. |
| title_short | Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells. |
| title_sort | inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060015&type=printable |
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