Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass

Six aristolochic acids were identified in the Chilean species Aristolochia chilensis using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) and subsequent confirmation with mass spectrometry (MS). The fractions of each signal were collected and injected directly in...

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Main Authors: Michael Araya, Samantha García, Marcia González-Teuber
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2018/5036542
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author Michael Araya
Samantha García
Marcia González-Teuber
author_facet Michael Araya
Samantha García
Marcia González-Teuber
author_sort Michael Araya
collection DOAJ
description Six aristolochic acids were identified in the Chilean species Aristolochia chilensis using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) and subsequent confirmation with mass spectrometry (MS). The fractions of each signal were collected and injected directly into an Orbitrap mass detector model Q Exactive Focus (Thermo Scientific). The acids extraction was done with 0.10–0.50 g of lyophilized and pulverized sample and concentrated in Soxhlet extraction equipment. The liquid-liquid separations and a subsequent solid phase extraction (SPE) C18 were performed using 100 µL of the extract that contains the aristolochic acids present in the Aristolochia chilensis plant. The HPLC conditions used a single mobile phase acetonitrile : water (1 : 1) acidified with 0.1% acetic acid and an isocratic elution to 1 mL·min−1. The column InertSustain C18 250 × 4.6 mm and 3 µm was used, the injection volume was 20 µL, and the time of run was reduced to 15 min. Calibration curves were constructed with r2 being 0.9997. The quantification limit for AAI was 0.138 ± 0.010 µg/mL, and for AAII, it was 0.558 ± 0.042 µg/mL.
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spelling doaj-art-d85eb24c658048d3aa438ed8f1a5174a2025-02-03T01:31:44ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/50365425036542Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited BiomassMichael Araya0Samantha García1Marcia González-Teuber2Centro de Investigación y Desarrollo Tecnológico de Algas (CIDTA), Facultad de Ciencias del Mar, Universidad Católica del Norte, Coquimbo, ChileDepartamento de Química, Facultad de Ciencias, Universidad de La Serena, La Serena, ChileLaboratorio de Química Ecológica, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileSix aristolochic acids were identified in the Chilean species Aristolochia chilensis using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) and subsequent confirmation with mass spectrometry (MS). The fractions of each signal were collected and injected directly into an Orbitrap mass detector model Q Exactive Focus (Thermo Scientific). The acids extraction was done with 0.10–0.50 g of lyophilized and pulverized sample and concentrated in Soxhlet extraction equipment. The liquid-liquid separations and a subsequent solid phase extraction (SPE) C18 were performed using 100 µL of the extract that contains the aristolochic acids present in the Aristolochia chilensis plant. The HPLC conditions used a single mobile phase acetonitrile : water (1 : 1) acidified with 0.1% acetic acid and an isocratic elution to 1 mL·min−1. The column InertSustain C18 250 × 4.6 mm and 3 µm was used, the injection volume was 20 µL, and the time of run was reduced to 15 min. Calibration curves were constructed with r2 being 0.9997. The quantification limit for AAI was 0.138 ± 0.010 µg/mL, and for AAII, it was 0.558 ± 0.042 µg/mL.http://dx.doi.org/10.1155/2018/5036542
spellingShingle Michael Araya
Samantha García
Marcia González-Teuber
Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
Journal of Analytical Methods in Chemistry
title Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
title_full Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
title_fullStr Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
title_full_unstemmed Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
title_short Rapid Identification and Simultaneous Quantification of Aristolochic Acids by HPLC-DAD and Confirmations by MS in Aristolochia chilensis Using a Limited Biomass
title_sort rapid identification and simultaneous quantification of aristolochic acids by hplc dad and confirmations by ms in aristolochia chilensis using a limited biomass
url http://dx.doi.org/10.1155/2018/5036542
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