Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts

The genus Achillea (Yarrow)is one of the most important medicinal plants. Nowadays, different medicinal functions of yarrow such as spasmolytic, choleretic, treatment of wounds and anti-inflammatory activities, make it as an important medicinal plant. The purpose of this study was to determine the a...

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Main Authors: Soulaf Mehlous, Smain Amira, Fatima Benchikh, Hassiba Benabdallah, Walid Mamache, Chawki Bensouici, Hocine Laouer, Karima Loucif
Format: Article
Language:English
Published: Hasan Eleroğlu 2022-10-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/5249
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author Soulaf Mehlous
Smain Amira
Fatima Benchikh
Hassiba Benabdallah
Walid Mamache
Chawki Bensouici
Hocine Laouer
Karima Loucif
author_facet Soulaf Mehlous
Smain Amira
Fatima Benchikh
Hassiba Benabdallah
Walid Mamache
Chawki Bensouici
Hocine Laouer
Karima Loucif
author_sort Soulaf Mehlous
collection DOAJ
description The genus Achillea (Yarrow)is one of the most important medicinal plants. Nowadays, different medicinal functions of yarrow such as spasmolytic, choleretic, treatment of wounds and anti-inflammatory activities, make it as an important medicinal plant. The purpose of this study was to determine the antioxidant activity of aqueous (AAE) and methanol (AME) extracts from the plant Achillea santolinoides L. (A. santolinoides L.) aerial parts in vitro. Quantitative evaluation of polyphenols, flavonoids and tannins were determined using Folin-Ciocalteu reagent, aluminum chloride (AlCl3) and Bate Smith methods, respectively. Antioxidant activity was carried out using ABTS radical scavenging, alkaline DMSO superoxide radical scavenging and cupric reducing antioxidant capacity (CUPRAC). The obtained results showed that the highest content in total phenolic, flavonoids and tannins was found in the AME with values of 210.78±0.001 µg GAE/mg Dw, 21.18±0.025 µg QE/mg DW and198.73±0.014µg TAE/mg DW, respectively. For the in vitro antioxidant activity, AME had the strongest ABTS and DMSO alkaline radical scavenging activity (IC50= 6.74±0.16 µg/mL and 15.13±0.92 µg/mL, respectively) and the CUPRAC reducing with A0.50 of 76.56±2.35 µg/mL. The results of the present study confirm the use of the genus Achillea in the treatment of various diseases as a powerful antioxidant.
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series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-d85ca6072d294ca9bb40966c41d32e412025-08-20T02:20:58ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-10-0110102045205010.24925/turjaf.v10i10.2045-2050.52492637Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial partsSoulaf Mehlous0Smain Amira1Fatima Benchikh2Hassiba Benabdallah3Walid Mamache4Chawki Bensouici5Hocine Laouer6Karima Loucif7Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University of Setif 1, AlgeriaLaboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University of Setif 1, AlgeriaLaboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University of Setif 1, AlgeriaLaboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University of Setif 1, AlgeriaLaboratory of Phytotherapy Applied to Chronic Diseases, Department of Biochemistry, Faculty of Nature and Life Sciences, University of Setif 1, 19000, AlgeriaCentre de Recherche en Biotechnologie (C.R.B.T), Constantine, AlgeriaLaboratory of Valorization of Natural Biological Resources, Department of Biology and Vegetal Ecology, University of Setif 1, AlgeriaLaboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University of Setif 1, AlgeriaThe genus Achillea (Yarrow)is one of the most important medicinal plants. Nowadays, different medicinal functions of yarrow such as spasmolytic, choleretic, treatment of wounds and anti-inflammatory activities, make it as an important medicinal plant. The purpose of this study was to determine the antioxidant activity of aqueous (AAE) and methanol (AME) extracts from the plant Achillea santolinoides L. (A. santolinoides L.) aerial parts in vitro. Quantitative evaluation of polyphenols, flavonoids and tannins were determined using Folin-Ciocalteu reagent, aluminum chloride (AlCl3) and Bate Smith methods, respectively. Antioxidant activity was carried out using ABTS radical scavenging, alkaline DMSO superoxide radical scavenging and cupric reducing antioxidant capacity (CUPRAC). The obtained results showed that the highest content in total phenolic, flavonoids and tannins was found in the AME with values of 210.78±0.001 µg GAE/mg Dw, 21.18±0.025 µg QE/mg DW and198.73±0.014µg TAE/mg DW, respectively. For the in vitro antioxidant activity, AME had the strongest ABTS and DMSO alkaline radical scavenging activity (IC50= 6.74±0.16 µg/mL and 15.13±0.92 µg/mL, respectively) and the CUPRAC reducing with A0.50 of 76.56±2.35 µg/mL. The results of the present study confirm the use of the genus Achillea in the treatment of various diseases as a powerful antioxidant.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5249achillea santolinoides l.polyphenolstanninsabtsalkaline dmso superoxidecuprac reducing.
spellingShingle Soulaf Mehlous
Smain Amira
Fatima Benchikh
Hassiba Benabdallah
Walid Mamache
Chawki Bensouici
Hocine Laouer
Karima Loucif
Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
Turkish Journal of Agriculture: Food Science and Technology
achillea santolinoides l.
polyphenols
tannins
abts
alkaline dmso superoxide
cuprac reducing.
title Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
title_full Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
title_fullStr Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
title_full_unstemmed Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
title_short Alkaline DMSO superoxide and radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and polyphenol contents of aqueous and methanol extract from Achilllea santolinoides L. aerial parts
title_sort alkaline dmso superoxide and radical scavenging cupric reducing antioxidant capacity cuprac and polyphenol contents of aqueous and methanol extract from achilllea santolinoides l aerial parts
topic achillea santolinoides l.
polyphenols
tannins
abts
alkaline dmso superoxide
cuprac reducing.
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/5249
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