Genome-wide association study on dairy goat milk production traits using three models

IntroductionIdentifying genetic markers associated with economically important traits in dairy goats helps enhance breeding efficiency, thereby increasing industry value. However, the potential genetic structure of key economic traits in dairy goats is still largely unknown.MethodsThis study used th...

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Main Authors: Zhengang Huang, Yuanping Tang, Jianyu Zhou, Dongliang Xu, Xiaokun Lin, Ming Cheng, Jianguang Wang, Qinan Zhao, Jianning He, Xiaoxiao Gao, Jinshan Zhao, Hegang Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Genetics
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Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2025.1650836/full
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author Zhengang Huang
Yuanping Tang
Jianyu Zhou
Dongliang Xu
Xiaokun Lin
Ming Cheng
Jianguang Wang
Qinan Zhao
Jianning He
Xiaoxiao Gao
Jinshan Zhao
Hegang Li
author_facet Zhengang Huang
Yuanping Tang
Jianyu Zhou
Dongliang Xu
Xiaokun Lin
Ming Cheng
Jianguang Wang
Qinan Zhao
Jianning He
Xiaoxiao Gao
Jinshan Zhao
Hegang Li
author_sort Zhengang Huang
collection DOAJ
description IntroductionIdentifying genetic markers associated with economically important traits in dairy goats helps enhance breeding efficiency, thereby increasing industry value. However, the potential genetic structure of key economic traits in dairy goats is still largely unknown.MethodsThis study used three genome-wide association study (GWAS) models (GLM, MLM, FarmCPU) to analyze dairy goat milk production traits (milk yield, fat percentage, protein percentage, lactose percentage, ash percentage, total dry matter, and somatic cell count). The goal was to identify SNPs and positional and functional candidate genes significantly associated with these traits.ResultsThe GWAS analysis results identified a total of 242 significant SNPs. Among these, 45 SNPs exhibited genome-wide significance, while 197 SNPs demonstrated suggestive associations, corresponding to 99 positional candidate genes within a 50 kb upstream and downstream range. 15 significant SNP loci were consistently identified across all three models, corresponding to 18 candidate genes.The integrated analysis of three models detected 2, 19, 17, 4, 115, 23, and 62 significant SNPs associated with milk yield, ash percentage, protein percentage, lactose percentage, somatic cell count, fat percentage, and total dry matter percentage, respectively. Correspondingly, 6, 24, 9, 12, 37, 14, and 30 candidate genes were identified for these traits. Additionally, several new candidate genes related to milk production traits were proposed (LCORL, TNFRSF1A, VWF, SPATA6, MAN1C1, MASP1, BRCA2).DiscussionIn summary, the results of this study provide an important reference for further exploration of the genetic mechanisms underlying dairy goat milk production traits and the development of molecular breeding markers.
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spelling doaj-art-d859f0a59e1e49fc8abe7a83c79f8a602025-08-22T04:10:35ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-08-011610.3389/fgene.2025.16508361650836Genome-wide association study on dairy goat milk production traits using three modelsZhengang Huang0Yuanping Tang1Jianyu Zhou2Dongliang Xu3Xiaokun Lin4Ming Cheng5Jianguang Wang6Qinan Zhao7Jianning He8Xiaoxiao Gao9Jinshan Zhao10Hegang Li11Qingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Institute of Animal Husbandry and Veterinary Medicine, Qingdao, ChinaInner Mongolia Shengjian Biotechnology Co., Ltd, Hohhot, ChinaInner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaQingdao Agricultural University, Qingdao, ChinaIntroductionIdentifying genetic markers associated with economically important traits in dairy goats helps enhance breeding efficiency, thereby increasing industry value. However, the potential genetic structure of key economic traits in dairy goats is still largely unknown.MethodsThis study used three genome-wide association study (GWAS) models (GLM, MLM, FarmCPU) to analyze dairy goat milk production traits (milk yield, fat percentage, protein percentage, lactose percentage, ash percentage, total dry matter, and somatic cell count). The goal was to identify SNPs and positional and functional candidate genes significantly associated with these traits.ResultsThe GWAS analysis results identified a total of 242 significant SNPs. Among these, 45 SNPs exhibited genome-wide significance, while 197 SNPs demonstrated suggestive associations, corresponding to 99 positional candidate genes within a 50 kb upstream and downstream range. 15 significant SNP loci were consistently identified across all three models, corresponding to 18 candidate genes.The integrated analysis of three models detected 2, 19, 17, 4, 115, 23, and 62 significant SNPs associated with milk yield, ash percentage, protein percentage, lactose percentage, somatic cell count, fat percentage, and total dry matter percentage, respectively. Correspondingly, 6, 24, 9, 12, 37, 14, and 30 candidate genes were identified for these traits. Additionally, several new candidate genes related to milk production traits were proposed (LCORL, TNFRSF1A, VWF, SPATA6, MAN1C1, MASP1, BRCA2).DiscussionIn summary, the results of this study provide an important reference for further exploration of the genetic mechanisms underlying dairy goat milk production traits and the development of molecular breeding markers.https://www.frontiersin.org/articles/10.3389/fgene.2025.1650836/fullgenome-wide association studycandidate genesmilk productiondairy goatsignificant SNPs
spellingShingle Zhengang Huang
Yuanping Tang
Jianyu Zhou
Dongliang Xu
Xiaokun Lin
Ming Cheng
Jianguang Wang
Qinan Zhao
Jianning He
Xiaoxiao Gao
Jinshan Zhao
Hegang Li
Genome-wide association study on dairy goat milk production traits using three models
Frontiers in Genetics
genome-wide association study
candidate genes
milk production
dairy goat
significant SNPs
title Genome-wide association study on dairy goat milk production traits using three models
title_full Genome-wide association study on dairy goat milk production traits using three models
title_fullStr Genome-wide association study on dairy goat milk production traits using three models
title_full_unstemmed Genome-wide association study on dairy goat milk production traits using three models
title_short Genome-wide association study on dairy goat milk production traits using three models
title_sort genome wide association study on dairy goat milk production traits using three models
topic genome-wide association study
candidate genes
milk production
dairy goat
significant SNPs
url https://www.frontiersin.org/articles/10.3389/fgene.2025.1650836/full
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