Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments
The diversity in anthocyanin flower pigmentation is vital in the ornamental plant market. To understand the regulation of the corona filament pigmentation of the <i>Passiflora</i> flower, we investigated the anthocyanin profiles of five distinct species (<i>P. violacea</i>, &...
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2025-03-01
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| author | Eliana Nutricati Erika Sabella Carmine Negro Samar Min Allah Andrea Luvisi Luigi De Bellis Rita Annunziata Accogli |
| author_facet | Eliana Nutricati Erika Sabella Carmine Negro Samar Min Allah Andrea Luvisi Luigi De Bellis Rita Annunziata Accogli |
| author_sort | Eliana Nutricati |
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| description | The diversity in anthocyanin flower pigmentation is vital in the ornamental plant market. To understand the regulation of the corona filament pigmentation of the <i>Passiflora</i> flower, we investigated the anthocyanin profiles of five distinct species (<i>P. violacea</i>, <i>P. caerulea</i>, <i>P. edulis</i>, <i>P. incarnata</i>, and <i>P. coccinea</i>) using HPLC-MS. A total of 14 anthocyanins, differentially distributed in the analyzed species, were identified as responsible for the differences in corona color, which can be attributed to different ratios of pelargonidin, cyanidin, and delphinidin. Additionally, we evaluated the expression of some biosynthetic genes, including <i>dehydroflavonol reductase</i> (<i>DFR), flavonoid 3′-hydroxylase (F3′H)</i>, and <i>flavonoid 3′,5′-hydroxylase (F3′5′H)</i>. <i>F3′H</i> seems to regulate the accumulation of cyanidins, <i>F3′5′H</i> determines blue pigmentation, and <i>DFR</i> enhances the biosynthesis of pelargonidins. Furthermore, three genes coding for key transcription factors, <i>Myeloblastosis (MYB)</i>, <i>basic helix-loop-helix (bHLH)</i>, and <i>WD repeat protein (WD40)</i>, were examined using qPCR. The results confirm that such genes regulate anthocyanin biosynthesis and provide insight into the molecular mechanisms that underlie pigment biosynthesis for application in biotechnologies. |
| format | Article |
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| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-d8562ac3bfdc48ee9bd12ec362a6d2ac2025-08-20T03:03:23ZengMDPI AGPlants2223-77472025-03-01147105010.3390/plants14071050Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona FilamentsEliana Nutricati0Erika Sabella1Carmine Negro2Samar Min Allah3Andrea Luvisi4Luigi De Bellis5Rita Annunziata Accogli6Department of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA), Salento University, Via Prov. le Lecce-Monteroni, 73100 Lecce, ItalyThe diversity in anthocyanin flower pigmentation is vital in the ornamental plant market. To understand the regulation of the corona filament pigmentation of the <i>Passiflora</i> flower, we investigated the anthocyanin profiles of five distinct species (<i>P. violacea</i>, <i>P. caerulea</i>, <i>P. edulis</i>, <i>P. incarnata</i>, and <i>P. coccinea</i>) using HPLC-MS. A total of 14 anthocyanins, differentially distributed in the analyzed species, were identified as responsible for the differences in corona color, which can be attributed to different ratios of pelargonidin, cyanidin, and delphinidin. Additionally, we evaluated the expression of some biosynthetic genes, including <i>dehydroflavonol reductase</i> (<i>DFR), flavonoid 3′-hydroxylase (F3′H)</i>, and <i>flavonoid 3′,5′-hydroxylase (F3′5′H)</i>. <i>F3′H</i> seems to regulate the accumulation of cyanidins, <i>F3′5′H</i> determines blue pigmentation, and <i>DFR</i> enhances the biosynthesis of pelargonidins. Furthermore, three genes coding for key transcription factors, <i>Myeloblastosis (MYB)</i>, <i>basic helix-loop-helix (bHLH)</i>, and <i>WD repeat protein (WD40)</i>, were examined using qPCR. The results confirm that such genes regulate anthocyanin biosynthesis and provide insight into the molecular mechanisms that underlie pigment biosynthesis for application in biotechnologies.https://www.mdpi.com/2223-7747/14/7/1050<i>Passiflora</i>anthocyanin profilesflower pigmentationanthocyanin biosynthetic pathwayflavonoids |
| spellingShingle | Eliana Nutricati Erika Sabella Carmine Negro Samar Min Allah Andrea Luvisi Luigi De Bellis Rita Annunziata Accogli Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments Plants <i>Passiflora</i> anthocyanin profiles flower pigmentation anthocyanin biosynthetic pathway flavonoids |
| title | Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments |
| title_full | Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments |
| title_fullStr | Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments |
| title_full_unstemmed | Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments |
| title_short | Anthocyanins and Anthocyanin Biosynthesis Gene Expression in <i>Passiflora</i> Flower Corona Filaments |
| title_sort | anthocyanins and anthocyanin biosynthesis gene expression in i passiflora i flower corona filaments |
| topic | <i>Passiflora</i> anthocyanin profiles flower pigmentation anthocyanin biosynthetic pathway flavonoids |
| url | https://www.mdpi.com/2223-7747/14/7/1050 |
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