Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola
Anthracnose, caused by Colletotrichum fructicola, is a serious disease of Buxus bodinieri. Microbial antagonists provide a novel approach for sustainable plant disease management. This study investigated the biocontrol efficacy of Bacillus velezensis HY13 against anthracnose in B. bodinieri and eluc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425001550 |
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| author | Ruiqi Peng Aiting Zhou Jianxin Chen Mingjia Wen Fang Wang Jianrong Wu |
| author_facet | Ruiqi Peng Aiting Zhou Jianxin Chen Mingjia Wen Fang Wang Jianrong Wu |
| author_sort | Ruiqi Peng |
| collection | DOAJ |
| description | Anthracnose, caused by Colletotrichum fructicola, is a serious disease of Buxus bodinieri. Microbial antagonists provide a novel approach for sustainable plant disease management. This study investigated the biocontrol efficacy of Bacillus velezensis HY13 against anthracnose in B. bodinieri and elucidated its underlying mechanisms. The results showed that B. velezensis HY13 significantly inhibited C. fructicola growth suppressed lesion expansion on B. bodinieri leaves in vitro. HY13 exhibited the ability to secrete extracellular hydrolytic enzymes, including chitinase (CHI) and β-1,3-glucanase (GLU), synthesize lipopeptides exhibiting antimicrobial, as well as form robust biofilms. Furthermore, HY13 treatment disrupted the cell membrane integrity of C. fructicola, leading to increased leakage of cellular contents from the fungal mycelium. In addition, anatomical examination of B. bodinieri leaves indicated that HY13 application enhanced leaf blade thickness (TLB), palisade parenchyma thicknesses (TPP), spongy parenchyma thicknesses (TSP) and lower epidermis thickness (TLE), thereby strengthening the physical barrier against pathogen invasion. Therefore, it is concluded that B. velezensis HY13 is a potent biocontrol agent for managing anthracnose in B. bodinieri. |
| format | Article |
| id | doaj-art-d8551fbeea524a4884d0a1e8fb903c77 |
| institution | DOAJ |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-d8551fbeea524a4884d0a1e8fb903c772025-08-20T03:03:01ZengElsevierBiological Control1049-96442025-09-0120810584510.1016/j.biocontrol.2025.105845Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicolaRuiqi Peng0Aiting Zhou1Jianxin Chen2Mingjia Wen3Fang Wang4Jianrong Wu5Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China; Forestry College, Southwest Forestry University, Kunming 650224, China; Corresponding authors at: Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China.Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China; Corresponding authors at: Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China.Anthracnose, caused by Colletotrichum fructicola, is a serious disease of Buxus bodinieri. Microbial antagonists provide a novel approach for sustainable plant disease management. This study investigated the biocontrol efficacy of Bacillus velezensis HY13 against anthracnose in B. bodinieri and elucidated its underlying mechanisms. The results showed that B. velezensis HY13 significantly inhibited C. fructicola growth suppressed lesion expansion on B. bodinieri leaves in vitro. HY13 exhibited the ability to secrete extracellular hydrolytic enzymes, including chitinase (CHI) and β-1,3-glucanase (GLU), synthesize lipopeptides exhibiting antimicrobial, as well as form robust biofilms. Furthermore, HY13 treatment disrupted the cell membrane integrity of C. fructicola, leading to increased leakage of cellular contents from the fungal mycelium. In addition, anatomical examination of B. bodinieri leaves indicated that HY13 application enhanced leaf blade thickness (TLB), palisade parenchyma thicknesses (TPP), spongy parenchyma thicknesses (TSP) and lower epidermis thickness (TLE), thereby strengthening the physical barrier against pathogen invasion. Therefore, it is concluded that B. velezensis HY13 is a potent biocontrol agent for managing anthracnose in B. bodinieri.http://www.sciencedirect.com/science/article/pii/S1049964425001550Buxus bodinieriAnthracnoseBiological controlBacillus velezensis |
| spellingShingle | Ruiqi Peng Aiting Zhou Jianxin Chen Mingjia Wen Fang Wang Jianrong Wu Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola Biological Control Buxus bodinieri Anthracnose Biological control Bacillus velezensis |
| title | Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola |
| title_full | Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola |
| title_fullStr | Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola |
| title_full_unstemmed | Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola |
| title_short | Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola |
| title_sort | antifungal activity of bacillus velezensis hy13 against anthracnose disease of buxus bodinieri caused by colletotrichum fructicola |
| topic | Buxus bodinieri Anthracnose Biological control Bacillus velezensis |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425001550 |
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