Antifungal activity of Bacillus velezensis HY13 against anthracnose disease of Buxus bodinieri caused by Colletotrichum fructicola

Anthracnose, caused by Colletotrichum fructicola, is a serious disease of Buxus bodinieri. Microbial antagonists provide a novel approach for sustainable plant disease management. This study investigated the biocontrol efficacy of Bacillus velezensis HY13 against anthracnose in B. bodinieri and eluc...

Full description

Saved in:
Bibliographic Details
Main Authors: Ruiqi Peng, Aiting Zhou, Jianxin Chen, Mingjia Wen, Fang Wang, Jianrong Wu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Biological Control
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1049964425001550
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anthracnose, caused by Colletotrichum fructicola, is a serious disease of Buxus bodinieri. Microbial antagonists provide a novel approach for sustainable plant disease management. This study investigated the biocontrol efficacy of Bacillus velezensis HY13 against anthracnose in B. bodinieri and elucidated its underlying mechanisms. The results showed that B. velezensis HY13 significantly inhibited C. fructicola growth suppressed lesion expansion on B. bodinieri leaves in vitro. HY13 exhibited the ability to secrete extracellular hydrolytic enzymes, including chitinase (CHI) and β-1,3-glucanase (GLU), synthesize lipopeptides exhibiting antimicrobial, as well as form robust biofilms. Furthermore, HY13 treatment disrupted the cell membrane integrity of C. fructicola, leading to increased leakage of cellular contents from the fungal mycelium. In addition, anatomical examination of B. bodinieri leaves indicated that HY13 application enhanced leaf blade thickness (TLB), palisade parenchyma thicknesses (TPP), spongy parenchyma thicknesses (TSP) and lower epidermis thickness (TLE), thereby strengthening the physical barrier against pathogen invasion. Therefore, it is concluded that B. velezensis HY13 is a potent biocontrol agent for managing anthracnose in B. bodinieri.
ISSN:1049-9644