The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep
Paratuberculosis (PTB), primarily caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), is a chronic infection that affects ruminants and is difficult to prevent, diagnose, and treat. Investigating how MAP infections affect the gut microbiota in sheep can...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/13/12/1118 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850102704402071552 |
|---|---|
| author | Shi-Yuan Xue Wei Ma Meng-Yuan Li Wei-Kang Meng Yu-Lin Ding Bo Yang Yue-Rong Lv Rui-Bin Chen Zhi-Hong Wu Siqin Tunala Rong Zhang Li Zhao Yong-Hong Liu |
| author_facet | Shi-Yuan Xue Wei Ma Meng-Yuan Li Wei-Kang Meng Yu-Lin Ding Bo Yang Yue-Rong Lv Rui-Bin Chen Zhi-Hong Wu Siqin Tunala Rong Zhang Li Zhao Yong-Hong Liu |
| author_sort | Shi-Yuan Xue |
| collection | DOAJ |
| description | Paratuberculosis (PTB), primarily caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), is a chronic infection that affects ruminants and is difficult to prevent, diagnose, and treat. Investigating how MAP infections affect the gut microbiota in sheep can aid in the prevention and treatment of ovine PTB. This study examined fecal samples from eight small-tail Han sheep (STHS) at various stages of infection and from three different field areas. All samples underwent DNA extraction and 16S rRNA sequencing. Among all samples, the phyla p. Firmicutes and p. Bacteroidota exhibited the highest relative abundance. The dominant genera in groups M1–M6 were UCG-005, <i>Christensenellaceae</i>_R-7_group, <i>Rikenellaceae</i>_RC9_gut_group, <i>Akkermansia</i>, UCG-005, and <i>Bacteroides</i>, whereas those in groups A–C were <i>Christensenellaceae</i>_R-7_group, <i>Escherichia–Shigella</i>, and <i>Acinetobacter</i>, respectively. The microbial community structure varied significantly among groups M1–M6. Specifically, 56 microbiota consortia with different taxonomic levels, including the order Clostridiales, were significantly enriched in groups M1–M6, whereas 96 microbiota consortia at different taxonomic levels, including the family <i>Oscillospiraceae</i>, were significantly enriched in groups A–C. To the best of our knowledge, this is the first study to report that MAP infection alters the intestinal microbiota of STHS. Changes in p. Firmicutes abundance can serve as a potential biomarker to distinguish MAP infection and determine the infection stage for its early diagnosis. Our study provides a theoretical basis for the treatment of PTB by regulating the intestinal microbiota, including p. Firmicutes. |
| format | Article |
| id | doaj-art-d845eb3077ea44e9ad06226d64c80d01 |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-d845eb3077ea44e9ad06226d64c80d012025-08-20T02:39:41ZengMDPI AGPathogens2076-08172024-12-011312111810.3390/pathogens13121118The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han SheepShi-Yuan Xue0Wei Ma1Meng-Yuan Li2Wei-Kang Meng3Yu-Lin Ding4Bo Yang5Yue-Rong Lv6Rui-Bin Chen7Zhi-Hong Wu8Siqin Tunala9Rong Zhang10Li Zhao11Yong-Hong Liu12College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaAnimal Disease Control Center of Ordos, Ordos 017000, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaOtok Banner Animal Disease Prevention and Control Center, Ordos 017000, ChinaAgriculture and Animal Husbandry Technology Popularization Center of Inner Mongolia Autonomous Region, Hohhot 010010, ChinaOtok Banner Animal Disease Prevention and Control Center, Ordos 017000, ChinaOtok Banner Animal Disease Prevention and Control Center, Ordos 017000, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaParatuberculosis (PTB), primarily caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), is a chronic infection that affects ruminants and is difficult to prevent, diagnose, and treat. Investigating how MAP infections affect the gut microbiota in sheep can aid in the prevention and treatment of ovine PTB. This study examined fecal samples from eight small-tail Han sheep (STHS) at various stages of infection and from three different field areas. All samples underwent DNA extraction and 16S rRNA sequencing. Among all samples, the phyla p. Firmicutes and p. Bacteroidota exhibited the highest relative abundance. The dominant genera in groups M1–M6 were UCG-005, <i>Christensenellaceae</i>_R-7_group, <i>Rikenellaceae</i>_RC9_gut_group, <i>Akkermansia</i>, UCG-005, and <i>Bacteroides</i>, whereas those in groups A–C were <i>Christensenellaceae</i>_R-7_group, <i>Escherichia–Shigella</i>, and <i>Acinetobacter</i>, respectively. The microbial community structure varied significantly among groups M1–M6. Specifically, 56 microbiota consortia with different taxonomic levels, including the order Clostridiales, were significantly enriched in groups M1–M6, whereas 96 microbiota consortia at different taxonomic levels, including the family <i>Oscillospiraceae</i>, were significantly enriched in groups A–C. To the best of our knowledge, this is the first study to report that MAP infection alters the intestinal microbiota of STHS. Changes in p. Firmicutes abundance can serve as a potential biomarker to distinguish MAP infection and determine the infection stage for its early diagnosis. Our study provides a theoretical basis for the treatment of PTB by regulating the intestinal microbiota, including p. Firmicutes.https://www.mdpi.com/2076-0817/13/12/1118<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i>small-tail Han sheepmicrobial populationhigh-throughput sequencingChina |
| spellingShingle | Shi-Yuan Xue Wei Ma Meng-Yuan Li Wei-Kang Meng Yu-Lin Ding Bo Yang Yue-Rong Lv Rui-Bin Chen Zhi-Hong Wu Siqin Tunala Rong Zhang Li Zhao Yong-Hong Liu The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep Pathogens <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> small-tail Han sheep microbial population high-throughput sequencing China |
| title | The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep |
| title_full | The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep |
| title_fullStr | The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep |
| title_full_unstemmed | The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep |
| title_short | The Impact of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> on Intestinal Microbial Community Composition and Diversity in Small-Tail Han Sheep |
| title_sort | impact of i mycobacterium avium i subsp i paratuberculosis i on intestinal microbial community composition and diversity in small tail han sheep |
| topic | <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> small-tail Han sheep microbial population high-throughput sequencing China |
| url | https://www.mdpi.com/2076-0817/13/12/1118 |
| work_keys_str_mv | AT shiyuanxue theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT weima theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT mengyuanli theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT weikangmeng theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yulinding theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT boyang theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yueronglv theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT ruibinchen theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT zhihongwu theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT siqintunala theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT rongzhang theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT lizhao theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yonghongliu theimpactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT shiyuanxue impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT weima impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT mengyuanli impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT weikangmeng impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yulinding impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT boyang impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yueronglv impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT ruibinchen impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT zhihongwu impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT siqintunala impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT rongzhang impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT lizhao impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep AT yonghongliu impactofimycobacteriumaviumisubspiparatuberculosisionintestinalmicrobialcommunitycompositionanddiversityinsmalltailhansheep |