Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori
Helicobacter pylori-infected gastrointestinal mucosa is frequently infiltrated by polymorphonuclear leukocytes (PMN) and monocytes, and these invading cells have been implicated in gastrointestinal mucosal inflammation. To clarify the efficacy of polaprezinc, a chelate compound consisting of zinc an...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2002-01-01
|
| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2002/631070 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550661199233024 |
|---|---|
| author | Osamu Handa Norimasa Yoshida Yukiko Tanaka Miho Ueda Takeshi Ishikawa Tomohisa Takagi Naoyuki Matsumoto Yuji Naito Toshikazu Yoshikawa |
| author_facet | Osamu Handa Norimasa Yoshida Yukiko Tanaka Miho Ueda Takeshi Ishikawa Tomohisa Takagi Naoyuki Matsumoto Yuji Naito Toshikazu Yoshikawa |
| author_sort | Osamu Handa |
| collection | DOAJ |
| description | Helicobacter pylori-infected gastrointestinal mucosa is frequently infiltrated by polymorphonuclear leukocytes (PMN) and monocytes, and these invading cells have been implicated in gastrointestinal mucosal inflammation. To clarify the efficacy of polaprezinc, a chelate compound consisting of zinc and L-carnosine, against H pylori-induced inflammation including PMN infiltration, the in vitro effects of this drug on interleukin (IL)-8 production by an established gastric cancer cell line (MKN 45 cells) and on PMN-endothelial cell adhesive interactions was investigated. Polaprezinc and zinc sulphate inhibited IL-8 production by MKN 45 cells in response to stimulation with H pylori water extract (HPE) in a dose-dependent manner from 10-7 M to 10-5 M. In addition, the expression of CD11b and CD18 on PMN and PMN-dependent adhesion to endothelial cells elicited by HPE was inhibited by polaprezinc and zinc sulphate in a concentration-dependent manner. L-carnosine did not have any effects on IL-8 production or PMN-endothelial cell interactions. These results suggest that polaprezinc, mainly the zinc component, may inhibit H pylori-induced PMN-mediated gastric inflammation by attenuating CD11b/CD18 expression on PMN and IL-8 production from gastric epithelial cells. |
| format | Article |
| id | doaj-art-d83394f4e9704347a4092a395b9a4aca |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-d83394f4e9704347a4092a395b9a4aca2025-02-03T06:06:17ZengWileyCanadian Journal of Gastroenterology0835-79002002-01-01161178578910.1155/2002/631070Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pyloriOsamu Handa0Norimasa Yoshida1Yukiko Tanaka2Miho Ueda3Takeshi Ishikawa4Tomohisa Takagi5Naoyuki Matsumoto6Yuji Naito7Toshikazu Yoshikawa8First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanFirst Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, JapanHelicobacter pylori-infected gastrointestinal mucosa is frequently infiltrated by polymorphonuclear leukocytes (PMN) and monocytes, and these invading cells have been implicated in gastrointestinal mucosal inflammation. To clarify the efficacy of polaprezinc, a chelate compound consisting of zinc and L-carnosine, against H pylori-induced inflammation including PMN infiltration, the in vitro effects of this drug on interleukin (IL)-8 production by an established gastric cancer cell line (MKN 45 cells) and on PMN-endothelial cell adhesive interactions was investigated. Polaprezinc and zinc sulphate inhibited IL-8 production by MKN 45 cells in response to stimulation with H pylori water extract (HPE) in a dose-dependent manner from 10-7 M to 10-5 M. In addition, the expression of CD11b and CD18 on PMN and PMN-dependent adhesion to endothelial cells elicited by HPE was inhibited by polaprezinc and zinc sulphate in a concentration-dependent manner. L-carnosine did not have any effects on IL-8 production or PMN-endothelial cell interactions. These results suggest that polaprezinc, mainly the zinc component, may inhibit H pylori-induced PMN-mediated gastric inflammation by attenuating CD11b/CD18 expression on PMN and IL-8 production from gastric epithelial cells.http://dx.doi.org/10.1155/2002/631070 |
| spellingShingle | Osamu Handa Norimasa Yoshida Yukiko Tanaka Miho Ueda Takeshi Ishikawa Tomohisa Takagi Naoyuki Matsumoto Yuji Naito Toshikazu Yoshikawa Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori Canadian Journal of Gastroenterology |
| title | Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori |
| title_full | Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori |
| title_fullStr | Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori |
| title_full_unstemmed | Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori |
| title_short | Inhibitory Effects of Polaprezine on the Inflammatory Response to Helicobacter pylori |
| title_sort | inhibitory effects of polaprezine on the inflammatory response to helicobacter pylori |
| url | http://dx.doi.org/10.1155/2002/631070 |
| work_keys_str_mv | AT osamuhanda inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT norimasayoshida inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT yukikotanaka inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT mihoueda inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT takeshiishikawa inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT tomohisatakagi inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT naoyukimatsumoto inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT yujinaito inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori AT toshikazuyoshikawa inhibitoryeffectsofpolaprezineontheinflammatoryresponsetohelicobacterpylori |