Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer
Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) has become a new hope for Alzheimer's disease due to its capability to suppress Amyloid-beta (Aβ) plaques formation. It is found that people with Alzheimer's disease have a lower expression of Sirtuin-1 because of the oxidative stress th...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Medicine at Universitas Andalas
2023-07-01
|
| Series: | Jurnal Kesehatan Andalas |
| Online Access: | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/2140 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545200051847168 |
|---|---|
| author | Havina Nurul Aizah Amel Yanis Hirowati Ali Tofrizal Tofrizal Cimi Ilmiawati Dessy Arisanty |
| author_facet | Havina Nurul Aizah Amel Yanis Hirowati Ali Tofrizal Tofrizal Cimi Ilmiawati Dessy Arisanty |
| author_sort | Havina Nurul Aizah |
| collection | DOAJ |
| description | Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) has become a new hope for Alzheimer's disease due to its capability to suppress Amyloid-beta (Aβ) plaques formation. It is found that people with Alzheimer's disease have a lower expression of Sirtuin-1 because of the oxidative stress that was produced by Aβ plaques. Objective: To observe the Sirtuin-1 gene in Alzheimer's disease rat models that AlCl3 was inducing after being given MSC-WJ. Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. Mean value of the Sirtuin-1 gene was obtained by the Image-J application using a semiquantitative method which was then tested using the One Way ANOVA test. The study was significant if the p-value < 0,05. Results: This study found that the mean ± standard error value of the Sirtuin-1 gene in three groups were 0.18±0.04; 0.16±0.03; and 0.36±0.06 respectively. The statistical test showed a significant difference between the three groups (p = 0.014). Conclusion: There was an increased Sirtuin 1 gene expression in Alzheimer’s rat Model after being given Mesenchymal Stem Cells Wharton’s Jelly.
Keywords: Alzheimer, MSC-WJ, Sirtuin-1 |
| format | Article |
| id | doaj-art-d82e567c76fe43de86269c3580d888c3 |
| institution | Kabale University |
| issn | 2301-7406 2615-1138 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Faculty of Medicine at Universitas Andalas |
| record_format | Article |
| series | Jurnal Kesehatan Andalas |
| spelling | doaj-art-d82e567c76fe43de86269c3580d888c32025-02-03T08:18:06ZengFaculty of Medicine at Universitas AndalasJurnal Kesehatan Andalas2301-74062615-11382023-07-0112191410.25077/jka.v12i1.21401235Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model AlzheimerHavina Nurul Aizah0Amel Yanis1Hirowati Ali2Tofrizal Tofrizal3Cimi Ilmiawati4Dessy Arisanty5Program Studi Kedokteran, Fakultas Kedokteran, Universitas AndalasDepartemen Psikiatri, Fakultas Kedokteran, Universitas AndalasDepartemen Biokimia, Fakultas Kedokteran, Universitas AndalasDepartemen Patologi Anatomi, Fakultas Kedokteran, Universitas AndalasDepartemen Farmakologi, Fakultas Kedokteran, Universitas AndalasDepartemen Biokimia, Fakultas Kedokteran, Universitas AndalasMesenchymal Stem Cells Wharton's Jelly (MSC-WJ) has become a new hope for Alzheimer's disease due to its capability to suppress Amyloid-beta (Aβ) plaques formation. It is found that people with Alzheimer's disease have a lower expression of Sirtuin-1 because of the oxidative stress that was produced by Aβ plaques. Objective: To observe the Sirtuin-1 gene in Alzheimer's disease rat models that AlCl3 was inducing after being given MSC-WJ. Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. Mean value of the Sirtuin-1 gene was obtained by the Image-J application using a semiquantitative method which was then tested using the One Way ANOVA test. The study was significant if the p-value < 0,05. Results: This study found that the mean ± standard error value of the Sirtuin-1 gene in three groups were 0.18±0.04; 0.16±0.03; and 0.36±0.06 respectively. The statistical test showed a significant difference between the three groups (p = 0.014). Conclusion: There was an increased Sirtuin 1 gene expression in Alzheimer’s rat Model after being given Mesenchymal Stem Cells Wharton’s Jelly. Keywords: Alzheimer, MSC-WJ, Sirtuin-1http://jurnal.fk.unand.ac.id/index.php/jka/article/view/2140 |
| spellingShingle | Havina Nurul Aizah Amel Yanis Hirowati Ali Tofrizal Tofrizal Cimi Ilmiawati Dessy Arisanty Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer Jurnal Kesehatan Andalas |
| title | Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer |
| title_full | Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer |
| title_fullStr | Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer |
| title_full_unstemmed | Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer |
| title_short | Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer |
| title_sort | pengaruh pemberian mesenchymal stem cells wharton s jelly terhadap ekspresi gen sirtuin 1 pada tikus model alzheimer |
| url | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/2140 |
| work_keys_str_mv | AT havinanurulaizah pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer AT amelyanis pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer AT hirowatiali pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer AT tofrizaltofrizal pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer AT cimiilmiawati pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer AT dessyarisanty pengaruhpemberianmesenchymalstemcellswhartonsjellyterhadapekspresigensirtuin1padatikusmodelalzheimer |