M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov
Background. Kidney diseases occupy one of the leading places in the overall structure of morbidity and are a socially significant problem due to the increase in pediatric patients. An important role in homeostasis and progression of kidney diseases is played by cytokines, which perform a number of b...
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| Format: | Article |
| Language: | English |
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V. N. Karazin Kharkiv National University
2025-04-01
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| Series: | Journal of V. N. Karazin Kharkiv National University: Series Medicine |
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| Online Access: | https://ukrmedsci.com/index.php/visnyk/article/view/178 |
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| author | M.S. Myroshnychenko Yu.М. Mishyn М.М. Mishyna І.А. Маrchеnkо А.М. Burov |
| author_facet | M.S. Myroshnychenko Yu.М. Mishyn М.М. Mishyna І.А. Маrchеnkо А.М. Burov |
| author_sort | M.S. Myroshnychenko |
| collection | DOAJ |
| description | Background. Kidney diseases occupy one of the leading places in the overall structure of morbidity and are a socially significant problem due to the increase in pediatric patients. An important role in homeostasis and progression of kidney diseases is played by cytokines, which perform a number of biological functions and interact with other cells and tissues of the body.
Purpose – study of the impact of experimental maternal chronic inflammatory process of the genitourinary system of bacterial etiology on the cytokine status of the offspring.
Materials and Methods. The study material was blood serum from WAG rat born to mothers with chronic inflammatory process of the genitourinary system caused by uroisolates of Proteus mirabilis and Streptococcus pyogenes. The level of cytokines was determined using IXLA, ELISA in the laboratory «MED-EKO» in Kharkiv (license No. 2224 dated 01.10.2020). The results were analyzed using the Software Statistica 7.
Results. The levels of proinflammatory interleukins were high in all experimental groups and increased with age. It should be noted a feature: higher levels of IL-1b are observed in the group of offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Streptococcus pyogenes, of all age categories, and higher levels of IL-6 are observed in 7-day-old and 1-month-old rat offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Proteus mirabilis. When determining the content of proinflammatory IL-2 and IL-17, an imbalance was found: in terms of IL-2 content, an imbalance was observed in the offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Streptococcus pyogenes, and in terms of IL-17 levels, in the offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Proteus mirabilis.
Conclusions. It has been established that cytokine imbalance contributes to the development of nephropathology in offspring exposed prenatally to maternal chronic inflammatory processes caused by Streptococcus pyogenes and Proteus mirabilis. |
| format | Article |
| id | doaj-art-d82afa5b97d247e587abce42c9cfdf95 |
| institution | Kabale University |
| issn | 2313-6693 2313-2396 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | V. N. Karazin Kharkiv National University |
| record_format | Article |
| series | Journal of V. N. Karazin Kharkiv National University: Series Medicine |
| spelling | doaj-art-d82afa5b97d247e587abce42c9cfdf952025-08-20T03:26:29ZengV. N. Karazin Kharkiv National UniversityJournal of V. N. Karazin Kharkiv National University: Series Medicine2313-66932313-23962025-04-01332(53)16817710.26565/2313-6693-2025-53-01M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. BurovM.S. Myroshnychenko0https://orcid.org/0000-0002-6920-8374Yu.М. Mishyn1https://orcid.org/0000-0003-2226-2944М.М. Mishyna2https://orcid.org/0000-0001-6484-4198І.А. Маrchеnkо3https://orcid.org/0000-0001-5583-9768А.М. Burov4https://orcid.org/0000-0002-3236-7259Kharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, UkraineKharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, UkraineKharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, UkraineKharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, UkraineKharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, UkraineBackground. Kidney diseases occupy one of the leading places in the overall structure of morbidity and are a socially significant problem due to the increase in pediatric patients. An important role in homeostasis and progression of kidney diseases is played by cytokines, which perform a number of biological functions and interact with other cells and tissues of the body. Purpose – study of the impact of experimental maternal chronic inflammatory process of the genitourinary system of bacterial etiology on the cytokine status of the offspring. Materials and Methods. The study material was blood serum from WAG rat born to mothers with chronic inflammatory process of the genitourinary system caused by uroisolates of Proteus mirabilis and Streptococcus pyogenes. The level of cytokines was determined using IXLA, ELISA in the laboratory «MED-EKO» in Kharkiv (license No. 2224 dated 01.10.2020). The results were analyzed using the Software Statistica 7. Results. The levels of proinflammatory interleukins were high in all experimental groups and increased with age. It should be noted a feature: higher levels of IL-1b are observed in the group of offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Streptococcus pyogenes, of all age categories, and higher levels of IL-6 are observed in 7-day-old and 1-month-old rat offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Proteus mirabilis. When determining the content of proinflammatory IL-2 and IL-17, an imbalance was found: in terms of IL-2 content, an imbalance was observed in the offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Streptococcus pyogenes, and in terms of IL-17 levels, in the offspring that were prenatally exposed to the maternal chronic inflammatory process caused by Proteus mirabilis. Conclusions. It has been established that cytokine imbalance contributes to the development of nephropathology in offspring exposed prenatally to maternal chronic inflammatory processes caused by Streptococcus pyogenes and Proteus mirabilis.https://ukrmedsci.com/index.php/visnyk/article/view/178cytokine profile offspring chronic maternal inflammatory process of the genitourinary system |
| spellingShingle | M.S. Myroshnychenko Yu.М. Mishyn М.М. Mishyna І.А. Маrchеnkо А.М. Burov M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov Journal of V. N. Karazin Kharkiv National University: Series Medicine cytokine profile offspring chronic maternal inflammatory process of the genitourinary system |
| title | M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov |
| title_full | M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov |
| title_fullStr | M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov |
| title_full_unstemmed | M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov |
| title_short | M.S. Myroshnychenko, Yu.М. Mishyn, М.М. Mishyna, І.А. Маrchеnkо, А.М. Burov |
| title_sort | m s myroshnychenko yu м mishyn м м mishyna і а маrchеnkо а м burov |
| topic | cytokine profile offspring chronic maternal inflammatory process of the genitourinary system |
| url | https://ukrmedsci.com/index.php/visnyk/article/view/178 |
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